An optimal culture condition maintains human hepatocyte phenotype after long-term culture

Hepatology Research

Volumn 35, Issue 3, 2006, Pages 169-177

  Yamamoto, Naoki ^a   Wu, Jian ^a   Zhang, Yanhong ^a   Catana, Andreea M ^a   Cai, Hongbo ^b   Strom, Stephen ^b   Novikoff, Phyllis M ^c   Zern, Mark A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Cytochrome P450; Hepatocyte; Long term culture; Optimal culture condition

Indexed keywords

ALBUMIN; ALPHA 1 ANTITRYPSIN; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; GLYCOGEN; RNA; TRANSFERRIN;

ADULT; AGED; ARTICLE; CELL FUNCTION; CELL ISOLATION; CELL JUNCTION; CELL POLARITY; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; DNA SYNTHESIS; ELECTRON MICROSCOPY; ENZYME ACTIVITY; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; INTRAHEPATIC BILE DUCT; LIVER CELL; LIVER CELL CULTURE; MALE; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 33745699882     PISSN: 13866346     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Alpini G., Phillips J.O., Vroman B., and Larusso N.F. Recent advances in the isolation of liver cells. Hepatology 20 (1994) 494-513
2. Kost D.P., and Michalopoulos G.K. Effect of epidermal growth factor on the expression of protooncogenes c-myc and c-Ha-ras in short-term primary hepatocyte culture. J Cell Physiol 144 (1990) 122-127
3. Runge D., Michalopoulos G.K., Strom S.C., and Runge D.M. Recent advances in human hepatocyte culture systems. Biochem Biophys Res Commun 274 (2000) 1-3
4. Kono Y., Yang S., and Roberts E.A. Extended primary culture of human hepatocytes in a collagen gel sandwich system. In Vitro Cell Dev Biol Anim 33 (1997) 467-472
5. Block G.D., Locker J., Bowen W.C., et al. Population expansion, clonal growth, and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGFα in a chemically defined medium. J Cell Biol 132 (1996) 1133-1149
6. Bhatia S.N., Balis U.J., Yarmush M.L., and Toner M. Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and non-parenchymal cells. FASEB J 13 (1999) 1883-1900
7. Michalopoulos G.K., Bowen B., Nussler A.K., et al. Comparative analysis of mitogenic and morphogenic effects of HGF and EGF on rat and human hepatocytes maintained in collagen gels. J Cell Physiol 156 (1999) 443-452
8. Runge D., Runge D.M., Jager D., et al. Serum-free, long-term cultures of human hepatocytes: maintenance of cell morphology, transcription factors, and liver-specific functions. Biochem Biophys Res Commun 269 (2000) 46-53
9. Ferrini J.B., Pichard L., Domergue J., and Maurel P. Long-term primary cultures of adult human hepatocytes. Chem Bio Interact 107 (1997) 31-45
10. Katsura N., Ikai I., Mitaka T., et al. Long-term culture of primary human hepatocytes with preservation of proliferative capacity and differentiated functions. J Surg Res 106 (2002) 115-123
11. Kostrubsky V.E., Strom S.C., Hanson J., et al. Evaluation of hepatotoxic potential of drugs by inhibition of bile acid transport in cultured primary human hepatocytes and intact rats. Toxicol Sci 76 (2003) 220-228
12. Runge D.M., Runge D., Dorko K., et al. Epidermal growth factor-and hepatocyte growth factor-receptor activity in serum-free cultures of human hepatocytes. J Hepatol 30 (1999) 265-274
13. Michalopoulos G.K., Bowen W., Nussler A.K., et al. Comparative analysis of mitogenic and morphogenic effects of HGF and EGF on rat and human hepatocytes maintained in collagen gels. J Cell Physiol 156 (1993) 443-452
14. Clement B., Guillouzo C., Campion J.P., et al. A long-term co-culture of adult human hepatocytes with rat liver epithelial cells: modulation of albumin secretion and accumulation of extracellular material. Hepatology l4 (1984) 373-380
15. Shirahashi H., Wu J., Yamamoto N., et al. Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage. Cell Transplant 13 (2004) 197-211
16. Kostubusky V.E., Ramachandran V., Venkataramanam R., et al. The use of human hepatocyte culture to study the induction of cytochrome P-450. Drug Metab Dispos 27 (1999) 887-894
17. Wege H., He H.T., Chui M.S., et al. Telomerase reconstitution immortalizes human fetal hepatocytes without disrupting their differentiation potential. Gastroenterology 124 (2003) 432-444
18. Zhu J.L., Wu J., Frizell E., et al. Rapamycin inhibits hepatic stellate cell proliferation in vitro and limits fibrogenesis in an in vivo model of liver fibrosis. Gastroenterology 117 (1999) 1198-1204
19. Kelly J., and Sussman N. A fluorescent cell based assay for cytochrome P-4501A2 induction and inhibition. J Biomol Screen 5 (2000) 249-254
20. Rambhatla L., Chiu C.P., Kundu P., et al. Generation of hepatocyte-like cells from human embryonic stem cells. Cell Transplant 12 (2003) 1-11
21. Karnovsky M.J. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27 (1965) 137A
22. In: Hayat M.A. (Ed). Principles and techniques of electron microscopy biological applications. 3rd ed. (1989), CRC Press, Inc., Boca Raton, FL 271-273
23. Sasaki A.W., Williams S.K., Jain M., and Wagner R.C. Mechanism of sucrose uptake by isolated rat hepatocytes. J Cell Physiol 133 (1987) 175-180
24. Nagata T. Three-dimensional high voltage electron microscopy of thick biological specimens. Micron 32 (2001) 387-404
25. Jefferson D.M., Reid L.M., Giambrone M.A., et al. Effects of dexamethasone on albumin and collagen gene expression in primary cultures of adult rat hepatocytes. Hepatology 5 (1985) 14-20
26. Woodworth C.D., and Isom H.C. Regulation of albumin gene expression in a series of rat hepatocyte cell lines immortalized by simian virus 40 and maintained in chemically defined medium. Mol Cell Biol 7 (1987) 3740-3748
27. Kim K.H., Han S.B., Hyun B.H., et al. Functional human hepatocyte isolation from small liver biopsy samples and primary cultivation with liver-specific functions. J Toxicol Sci 20 (1995) 565-578
28. Klein H.H., Ullmann S., Drenckhan M., et al. Differential modulation of insulin actions by dexamethasone: studies in primary cultures of adult rat hepatocytes. J Hepatol 37 (2002) 432-440