C-terminal fluorescence labeling of proteins for interaction studies on the single-molecule level

ChemBioChem

Volumn 7, Issue 6, 2006, Pages 891-895

  Becker, Christian F W ^a   Seidel, Ralf ^a   Jahnz, Michael ^b   Bacia, Kirsten ^b   Niederhausen, Thomas ^b   Alexandrov, Kirill ^a   Schwille, Petra ^b   Goody, Roger S ^a   Engelhard, Martin ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Expressed protein ligation; Fluorescent labeling; FRET; Proteins; Single molecule studies

Indexed keywords

FLUORESCENT DYE; GUANOSINE TRIPHOSPHATASE; RAS PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; ONCOGENE H RAS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; MODELS, MOLECULAR; PROTEIN BINDING; PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 33745324492     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200500535     Document Type: Article

References (26)

1. R. David, M. P. Richter, A. G. Beck-Sickinger, Eur. J. Biochem. 2004, 271, 663-677.
2. T. W. Muir, Annu. Rev. Biochem. 2003, 72, 249-289.
3. P. E. Dawson, T. W. Muir, I. Clark-Lewis, S. B. H. Kent, Science 1994, 266, 776-779.
4. G. J. Cotton, T. W. Muir, Chem. Biol. 2000, 7, 253-261.
5. J. R. Sydor, C. Herrmann, S. B. Kent, R. S. Goody, M. Engelhard, Proc. Natl. Acad. Sci. USA 1999, 96, 7865-7870.
6. C. F. Becker, C. L. Hunter, R. P. Seidel, S. B. Kent, R. S. Goody, M. Engelhard, Chem. Biol. 2001, 8, 243-252.
7. T. Durek, K. Alexandrov, R. S. Goody, A. Hildebrand, I. Heinemann, H. Waldmann, J. Am. Chem. Soc. 2004, 126, 16368-16378.
8. M. Barbacid, Annu. Rev. Biochem. 1987, 56, 779-827.
9. A. Wittinghofer, H. Waldmann, Angew. Chem. 2000, 112, 4360-4383;
10. Angew. Chem. Int. Ed. 2000, 39, 4193-4214.
11. N. Mitin, K. L. Rossman, C. J. Der, Curr. Biol. 2005, 15, R563-R574.
12. T. Lazar, M. Gotte, D. Gallwitz, Trends Biochem. Sci. 1997, 22, 468-472.
13. Y. Jiang, S. Ferro-Novick, Proc. Natl. Acad. Sci. USA 1994, 91, 4377-4381.
14. M. R. Lackner, R. M. Kindt, P. M. Carroll, K. Brown, M. R. Cancilla, C. Chen, H. de Silva, Y. Franke, B. Guan, T. Heuer, T. Hung, K. Keegan, J. M. Lee, V. Manne, C. O'Brien, D. Parry, J. J. Perez-Villar, R. K. Reddy, H. Xiao, H. Zhan, M. Cockett, G. Plowman, K. Fitzgerald, M. Costa, P. Ross-Macdonald, Cancer Cell 2005, 7, 325-336.
15. P. Schwille, F. J. Meyer-Almes, R. Rigler, Biophys. J. 1997, 72, 1878-1886.
16. J. Schmitt, H. Hess, H. G. Stunnenberg, Mol. Biol. Rep. 1993, 18, 223-230.
17. T. W. Muir, D. Sondhi, P. A. Cole, Proc. Natl. Acad. Sci. USA 1998, 95, 6705-6710.
18. M. Villain, J. Vizzavona, K. Rose, Chem. Biol. 2001, 8, 673-679.
19. N. Nassar, G. Horn, C. Herrmann, A. Scherer, F. McCormick, A. Wittinghofer, Nature 1995, 375, 554-560.
20. J. R. Sydor, M. Engelhard, A. Wittinghofer, R. S. Goody, C. Herrmann, Biochemistry 1998, 37, 14292-14299.
21. A. Scherer, J. John, R. Linke, R. S. Goody, A. Wittinghofer, E. F. Pai, K. C. Homes, J. Mol. Biol. 1989, 206, 257-259.
22. S. A. Kim, K. G. Heinze, K. Bacia, M. N. Waxham, P. Schwille, Biophys. J. 2005, 88, 4319-4336.
23. B. Dursina, N. H. Thoma, V. Sidorovitch, A. Niculae, A. Iakovenko, A. Rak, S. Albert, A. C. Ceacareanu, R. Kolling, C. Herrmann, R. S. Goody, K. Alexandrov, Biochemistry 2002, 41, 6805-6816.
24. B. Schuler, L. K. Pannell, Bioconjugate Chem. 2002, 13, 1039-1043.
25. C. P. Hackenberger, C. T. Friel, S. E. Radford, B. Imperiali, J. Am. Chem. Soc. 2005, 127, 12882-12889.
26. I. Giriat, T. W. Muir, J. Am. Chem. Soc. 2003, 125, 7180-7181.