Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains

Biotechnology Letters

Volumn 28, Issue 12, 2006, Pages 937-941

  Qian, Jiangchao ^a   Cai, Xianpeng ^a   Chu, Ju ^a   Zhuang, Yingping ^a   Zhang, Siliang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Bacillus subtilis; Guanosine; Inosine; Pur operon; Pura

Indexed keywords

BACILLUS SUBTILIS; GUANOSINE; INOSINE; PUR OPERON; PURA;

BACTERIA; CELLS; DNA; ENZYMES; GENETIC ENGINEERING; MUTAGENS;

GENES;

ADENYLOSUCCINATE SYNTHASE; GUANOSINE; INOSINE; INOSINE PHOSPHATE; NUCLEOTIDE; PURINE; PURINE DERIVATIVE;

ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; OPERON; PROMOTER REGION;

ADENYLOSUCCINATE SYNTHASE; BACILLUS SUBTILIS; BASE SEQUENCE; GUANOSINE; INOSINE; INOSINE MONOPHOSPHATE; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEOTIDES; OPERON; PROMOTER REGIONS (GENETICS); PROMOTER REGIONS, GENETIC; PURINES;

BACILLUS SUBTILIS;

EID: 33745244247     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9020-z     Document Type: Article

References (16)

1. Bera AK, Zhu J, Zalkin H, Smith JL (2003) Functional dissection of the Bacillus subtilis purine operator site. J Bacteriol 185:4099-4109
2. Cai XP, Chen SX, Chu J, Zhuang YP, Zhang SL (2002) The optimization of guanosine fermentation based on process parameter correlation analysis. Acta Microbiol Sin (China) 42:232-235
3. Chen WP, Kuo TT (1993) A simple and rapid method for the preparation of gram-negative bacterial genomic DNA. Nucleic Acids Res 21:2260
4. Ebbole DJ, Zalkin H (1987) Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de Nove purine nucleotide synthesis. J Biol Chem 262:8274-8287
5. Ebbole DJ, Zalkin H (1988) Detection of pur operon attenuated mRNA and accumulated degradation intermediates in Bacillus subtilis. J Biol Chem 263:10894-10902
6. Ebbole DJ, Zalkin H (1989) Bacillus subtilis pur operon expression and regulation. J Bacteriol 171:2136-2141
7. Matsui H, Sato K, Enei H, Hirose Y (1977) Mutation of an inosine-producing strain of Bacillus subtilis to DL-methionine sulfoxide resistance for guanosine production. Appl Environ Microbiol 34: 337-341
8. Miyagawa K, Doi M, Akiyama S (1984) Fermentation preparation of inosine and/or guanosine. Patent: GB 2124225
9. Miyagawa K, Kimura H, Nakahama K, Kikuchi M, Doi M, Akiyama S, Nakao Y (1986) Cloning of the Bacillus subtilis IMP dehydrogenase gene and its application to increased production of guanosine. Bio/Technology 4:225-228
10. Miyagawa K, Kanzaki N, Sumino Y (1988) Enhanced manufacture of inosine and guanosine with recombinant Bacillus. Patent: EP 286303
11. Miyagawa K, Kanzaki N (1991) Recombinant Bacillus with altered promoter for enhanced inosine and/or guanosine manufacture. Patent: EP 412688
12. Nishikawa H, Momose H, Shiio I (1967) Regulation of purine nucleotidesynthesis in Bacillus subtilis. J Biochem 62:92-98
13. Sumino Y, Sonoi K, Doi M (1983) An improved method for the production of nucleosides. EP 80864
14. Weng M, Nagy PL, Zalkin H (1995) Identification of the Bacillus subtilis pur operon repressor. Proc Natl Acad Sci USA 92:7455-7459
15. Weng M, Zalkin H (2000) Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo. Curr Microbiol 41:56-59
16. Xuan JS, Zalkin H, Weng M (2005) Mutations in PurBox1 of the Bacillus subtilis pur operon control site affect adenine-regulated expression in vivo. Sci China Ser. C Life Sci 48:133-138