Enhancement and selective production of oligomycin through inactivation of avermectin's starter unit in Streptomyces avermitilis

Biotechnology Letters

Volumn 28, Issue 12, 2006, Pages 911-916

  Wei, Xiong ^a,b   Yunxiang, Liang ^a   Yinghua, Zheng ^b  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b Beijing Institute of Biomedicine   (China)

Author keywords

Avermectin; Branched chain keto acid dehydrogenase; Oligomycin; PCR targeted gene disruption; Streptomyces avermitilis

Indexed keywords

AVERMECTIN; BRANCHED-CHAIN Α-KETO ACID DEHYDROGENASE; OLIGOMYCIN; PCR TARGETED GENE DISRUPTION; STREPTOMYCES AVERMITILIS;

BACTERIA; ENZYMES; GENES; GENETIC ENGINEERING; MUTAGENESIS;

ANTIBIOTICS;

AVERMECTIN; DRUG DERIVATIVE; IVERMECTIN; OLIGOMYCIN;

ARTICLE; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; STREPTOMYCES;

GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; INDUSTRIAL MICROBIOLOGY; IVERMECTIN; OLIGOMYCINS; POLYMERASE CHAIN REACTION; STREPTOMYCES;

ACTINOBACTERIA; ACTINOBACTERIA (CLASS); STREPTOMYCES; STREPTOMYCES AVERMITILIS;

EID: 33745226443     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9012-z     Document Type: Article

References (13)

1. Denoya CD, Fedechko RW, Hafner EW, McArthur HA, Morgenstern MR, Skinner DD, Stutzman-Engwall K, Wax RG, Wernau WC (1995) A second branched-chain alpha-keto acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis: its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production of novel antiparasitic avermectins. J Bacteriol 177:3504-3511
2. Gust B, Challis GL, Fowler K, Kieser T, Chater KF (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci 100:1541-1546
3. Ikeda H, Ishikawa J, Hanamoto A, Shinose M, Kikuchi H, Shiba T, Sakaki Y, Hattori M, Ōmura S (2003) Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat Biotechnol 21:526-531
4. Janssen GR, Bibb MJ (1993) Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124:133-134
5. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical streptomyces genetics. The John Innes Foundation, Norwich, UK
6. Korystov YN, Kublik LN, Kudryavtsev AA, Levitman MK, Shaposhnikova VV, Drinyaev VA, Mosin VA, Kruglyak EB, Sterlina TS (2003) Opposite Effects of low oligomycin concentrations on the apoptosis of normal and tumor cells. Doklady Biol Sci 392:475-477
7. Laatsch H, Kellner M, Wolf G, Lee YS, Hansske F, Konetschny-Rapp S, Pessara U, Scheuer W, Stockinger H (1993) Oligomycin F, a new immunosuppressive homologue of oligomycin A. J Antibiotics 46:1334-1341
8. Masamune S, Sehgal JM, Tamelen EE, Strong FM, Peterson (1958) Separation and preliminary characterization of oligomycins A, B and C. J. Am. Chem. Soc. 80:6092-6095
9. Ōmura S, Ikeda H, Ishikawa J, Hanamoto A, Takahashi C, Shinose M, Takahashi Y, Horikawa H, Nakazawa H, Osonoe T, Kikuchi H, Shiba T, Sakaki Y, Hattori M (2001) Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. Proc Natl Acad Sci 98:12215-12220
10. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, NY
11. Tzagoloff A, Meagher P (1972) Assesmbly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J Biol Chem 247:594-603
12. Yamazaki M, Yamashita T, Harada T, Nishikiori T, Saito S, Shimada N, Fujii A (1992) 44-Homooligomycins A and B, new antitumor antibiotics from Streptomyces bottropensis: producing organism, fermentation, isolation, structure elucidation and biological properties. J Antibiotics 45:171-179
13. Zhang XL, Chen Z, Zhao JL, Song Y, Wen Y, Li JL (2004) Deletion analysis of oligomycin PKS genes (olmA) in Streptomyces avermitilis. Chin Sci Bull 49:350-354