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Volumn 300, Issue 1, 2006, Pages 117-122

Facile fabrication of poly(p-phenylene ethynylene)/colloidal silica composite for nucleic acid detection

Author keywords

Fluorescence quenching; Nanoparticle; Nucleic acid detection; Poly(phenylene ethynylene); Stern Volmer analysis

Indexed keywords

BIOSENSORS; HYDROPHOBICITY; NANOSTRUCTURED MATERIALS; NUCLEIC ACID SEQUENCES; OLIGOMERS; QUANTUM EFFICIENCY; SILICA;

EID: 33745214895     PISSN: 00219797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcis.2006.03.063     Document Type: Article
Times cited : (16)

References (27)
  • 20
    • 33745220812 scopus 로고    scopus 로고
    • note
    • QY was measured using 9,10-diphenylanthracene in cyclohexane as a reference. QY of the composite in water could not be determined due to the interference of light scattering from aggregated particles in water.
  • 21
    • 33745222139 scopus 로고    scopus 로고
    • note
    • The composite was passed through a commercially available chamber (Microfluidizer M-110S, Microfluidics, Newton, MA) at 23,000 psi. The system utilizes high-pressure streams that collide at ultrahigh velocities in precisely defined microchannels.
  • 22
    • 33745192670 scopus 로고    scopus 로고
    • note
    • Total weight fraction of P1 was estimated based on elemental analysis results (% C: 7.12, H: 1.70, N: 2.29, and Si: 41.22). As a control, we fabricated a aminosilane/silica material with no P1. Elemental analysis indicated relatively low carbon (5.35%) and nitrogen (1.70%) content compared to the original P1/silica composite. Our calculated weight fraction of aminosilane based on the amount of nitrogen in the elemental analysis was 8.9-10.7 wt%. The calculated amount of aminosilane in the control material was well in accordance with the aminosilane fraction of the P1/silica composite (8.5-11.5 wt%).
  • 23
    • 33745207853 scopus 로고    scopus 로고
    • note
    • 2/g.
  • 25
    • 33745206978 scopus 로고    scopus 로고
    • note
    • -7 M), while the false target at the same concentration produced only 4% quenching. We compared quenching ability of Cy5 and BHQ1 and found no significant difference between them.
  • 27
    • 33745190341 scopus 로고    scopus 로고
    • note
    • We adjusted the probe density by transferring maleimide functionalized complex into a mixed solution of thiol-modified oligonucleotide: random 12-mers and specific 29-mers (1:1 molar concentration). Fast hybridization at the composites with adjusted probe density results in increased fluorescence quenching within the hybridization time. Details on the probe density effect on the quenching efficiency will be published soon.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.