DNA Damage-Induced Phosphorylation of Rad55 Protein as a Sentinel for DNA Damage Checkpoint Activation in S. cerevisiae

Methods in Enzymology

Volumn 409, Issue , 2006, Pages 166-182

  Bashkirov, Vladimir I ^a   Herzberg, Kristina ^a   Haghnazari, Edwin ^a   Vlasenko, Alexey S ^a   Heyer, Wolf Dietrich ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ATM PROTEIN; ATR PROTEIN; CHECKPOINT KINASE 2; CHECKPOINT KINASE MEC1; FUNGAL PROTEIN; PHOSPHOTRANSFERASE; RAD 55 PROTEIN; RAD 57 PROTEIN; UNCLASSIFIED DRUG;

CONTROLLED STUDY; DNA DAMAGE; ENZYME SUBSTRATE; GEL MOBILITY SHIFT ASSAY; GENETIC TRANSDUCTION; GENOTOXICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN TARGETING; REVIEW; SACCHAROMYCES CEREVISIAE; STRESS; YEAST;

CELL CYCLE PROTEINS; DIMERIZATION; DNA DAMAGE; DNA-BINDING PROTEINS; IMMUNOPRECIPITATION; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 33745198662     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)09010-5     Document Type: Review

References (17)

1. Bashkirov V.I., Bashkirova E.V., Haghnazari E., and Heyer W.D. Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase. Mol. Cell. Biol. 23 (2003) 1441-1452
2. Bashkirov V.I., King J.S., Bashkirova E.V., Schmuckli-Maurer J., and Heyer W.D. DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints. Mol. Cell. Biol. 20 (2000) 4393-4404
3. Carr A.M. DNA structure dependent checkpoints as regulators of DNA repair. DNA Repair 1 (2002) 983-994
4. Gilbert C.S., Green C.M., and Lowndes N.F. Budding yeast Rad9 is an ATP-dependent Rad53 activating machine. Mol. Cell 8 (2001) 129-136
5. Kim S.T., Lim D.S., Canman C.E., and Kastan M.B. Substrate specificities and identification of putative substrates of ATM kinase family members. J. Biol. Chem. 274 (1999) 37538-37543
6. Krogh B.O., and Symington L.S. Recombination proteins in yeast. Annu. Rev. Genet. 38 (2004) 233-271
7. Lee S.-J., Schwartz M.F., Duong J.K., and Stern D.F. Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. Mol. Cell. Biol. 23 (2003) 6300-6314
8. Mallory J.C., Bashkirov V.I., Trujillo K.M., Solinger J.A., Dominska M., Sung P., Heyer W.D., and Petes T.D. Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. DNA Repair 2 (2003) 1041-1064
9. Melo J., and Toczyski D. A unified view of the DNA-damage checkpoint. Curr. Opin. Cell Biol. 14 (2002) 237-245
10. Nelson J.R., Lawrence C.W., and Hinkle D.C. Thymine-thymine dimer bypass by yeast DNA polymerase zeta. Science 272 (1996) 1646-1649
11. Nyberg K.A., Michelson R.J., Putnam C.W., and Weinert T.A. Toward maintaining the genome: DNA damage and replication checkpoints. Annu. Review Genet. 36 (2002) 617-656
12. Pellicioli A., Lucca C., Liberi G., Marini F., Lopes M., Plevani P., Romano A., Di Fiore P.P., and Foiani M. Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase. EMBO J. 18 (1999) 6561-6572
13. Pringle J.R., Adams A.E.M., Drubin D.G., and Haarer B.K. Immunofluorescence methods for yeast. Methods Enzymol. 194 (1991) 565-602
14. Sanchez Y., Desany B.A., Jones W.J., Liu Q.H., Wang B., and Elledge S.J. Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. Science 271 (1996) 357-360
15. Sun Z.X., Fay D.S., Marini F., Foiani M., and Stern D.F. Spk1/Rad53 is regulated by Mec1-dependent protein phosphorylation in DNA replication and damage checkpoint pathways. Genes Dev. 10 (1996) 395-406
16. Sung P. Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase. Genes Devel. 11 (1997) 1111-1121
17. Zhou B.B.S., and Elledge S.J. The DNA damage response: Putting checkpoints in perspective. Nature 408 (2000) 433-439