Isolation and identification of mixed linked β-glucan degrading bacteria in the intestine of broiler chickens and partial characterization of respective 1,3-1,4-β-glucanase activities

Journal of Basic Microbiology

Volumn 46, Issue 3, 2006, Pages 175-185

  Beckmann, Lutz ^a,b   Simon, Ortwin ^a   Vahjen, Wilfried ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; BETA GLUCAN; GLYCOSIDASE; LICHENINASE; RNA 16S;

ANIMAL; ARTICLE; BACTEROIDES; CHICKEN; CLASSIFICATION; CLOSTRIDIUM PERFRINGENS; COMPARATIVE STUDY; ENTEROCOCCUS FAECIUM; ENZYMOLOGY; GENETICS; INTESTINE; ISOLATION AND PURIFICATION; MALE; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SPECIES DIFFERENCE; STREPTOCOCCUS BOVIS;

ANIMALS; BACTEROIDES; BETA-GLUCANS; CHICKENS; CLOSTRIDIUM PERFRINGENS; ENTEROCOCCUS FAECIUM; GLYCOSIDE HYDROLASES; INTESTINES; MALE; MOLECULAR SEQUENCE DATA; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SPECIES SPECIFICITY; STREPTOCOCCUS BOVIS;

BACTERIA (MICROORGANISMS); BACTEROIDES; BACTEROIDES (CLASS); BACTEROIDES CAPILLOSUS; BACTEROIDES OVATUS; BACTEROIDES UNIFORMIS; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; GALLUS GALLUS; STREPTOCOCCUS; STREPTOCOCCUS BOVIS; UNIDENTIFIED BACTERIUM;

EID: 33745172830     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.200510107     Document Type: Article

References (43)

1. BECKMANN, L., VAHJEN, W. and SIMON, O., 2000. Isolation of an 1,3-1,4-β-glucan degrading Enterococcus faecium strain from the intestinal tract of chicken and partial characterization of its novel 1,3-1,4-β- glucanase. J. Basic Microbiol., 40, 303-310.
2. BEDBURY, H. P. and DUKE, G. E., 1983. Cecal microflora of turkeys fed low or high fiber diets: enumeration, identification, and determination of cellulolytic activity. Poult. Sci., 62, 675-682.
3. BEDFORD, M. R., 2000. Exogenous enzymes in monogastric nutrition - their current value and future benefits. Anim. Feed Sci. Technol., 86, 1-13.
4. BRADFORD, M., 1976. A rapid sensitive method for the quantification of microorganism quantities of protein utilizing the principle protein-dye binding. Anal. Biochem., 72, 248-254.
5. BUCKEL, W., JANSSEN, P. H., SCHUHMANN, A., EIKSMANN, U., MESSNER, P., SLEYTR, U. and LIESACK, W., 1994. Clostridium viride sp. nov., a strictly anaerobic bacterium using 5-aminovalerate as growth substrate, previously assigned to Clostridium aminovalericum. Arch. Microbiol., 162, 387-394.
6. CATO, E. P., HOLDEMAN, L. V. and MOORE, W. E. C., 1979. Proposal of neotype strains for seven non-saccharolytic Bacteroides species. Int. J. Syst. Bacteriol., 29, 427-434.
7. COLOE, P. J., BAGUST, T. J. and IRELAND, L., 1984. Development of the normal gastrointestinal microflora of specific pathogen-free chickens. J. Hyg. (London), 92, 79-87.
8. CUMMINGS, J. H. and MACFARLANE, G. T., 1991. The control and consequences of bacterial fermentation in the human colon. J. Appl. Bacteriol., 70, 443-459.
9. DEGNAN, B. A., MACFARLANE, S. and MACFARLANE, G. T., 1997. Utilization of starch and synthesis of a combined amylase/alpha-glucosidase by the human colonic anaerobe Bacteroides ovatus. J. Appl. Microbiol., 83, 359-366.
10. DRASAR, B. S. and ROBERTS, A. K., 1991. Methods for the study of anaerobic microflora. In: Anaerobic Microbiology: a Practical Approach (P. N. LEVETT, Editor), pp. 183-200. Oxford University Press, New York, USA.
11. EKINCI, M. S., MCCRAE, S. I. and FLINT, H. J., 1997. Isolation and overexpression of a gene encoding an extracellular β-(1,3-1,4)-glucanase from Streptococcus bovis JB1. Appl. Environ. Microbiol., 63, 3752-3756.
12. ERFLE, J. D., TEATHER, R. M., WOOD, P. J. and IRVIN, J. E., 1988. Purification and properties of a 1,3-1,4-β-D-glucanase (lichenase, 1,3-1,4-β-D-glucan 4-glucanohydrolase, EC 3.2.1.73) from Bacteroides succinogenes cloned in Escherichia coli. Biochem. J., 255, 833-841.
13. FELIX, C. R. and LJUNGDAHL, L. G., 1993. The cellulosome: the exocellular organelle of Clostridium. Annu. Rev. Microbiol., 47, 791-819.
14. HEREDIA, A., JIMENEZ, A. and GUILLEN, R., 1995. Composition of plant cell walls. Z. Lebensm. Unters. For., 200, 24-31.
15. HUEBENER, K., VAHJEN, W. and SIMON, O., 2002. Bacterial responses to different dietary cereal types and xylanase supplementation in the intestine of broiler chicken. Arch. Anim. Nutr., 56, 167-187.
16. IRVIN, J. E. and TEATHER, R. M., 1988. Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli. Appl. Environ. Microbiol., 54, 2672-2676.
17. JONSSON, E. and HEMMINGSSON, S., 1991. Establishment in the piglet gut of lactobacilli capable of degrading mixed-linked β-glucans. J. Appl. Bacteriol., 70, 512-516.
18. KELLEY, R. W., 1983. Comparison of media for isolation of poultry intestinal bacteria. Appl. Environ. Microbiol., 46, 421-424.
19. LAN, P. T. N., HAYASHI, H., SAKAMOTO, M. and BENNO, Y., 2002. Phylogenetic analysis of cecal microbiota in chicken by the use of 16S rDNA clone libraries. Microbiol. Immunol., 46, 371-382.
20. LANE, D. J., 1991. 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics (E. STACKEBRANDT and M. GOODFELLOW, editors), pp. 115-175. John Wiley & Sons, Inc., New York, N.Y., USA.
21. MACFARLANE, G. T. and GIBSON, G. R., 1991. Co-utilization of polymerized carbon sources by Bacteroides ovatus grown in a two-stage continuous culture system. Appl. Environ. Microbiol., 57, 1-6.
22. MILLER, G. L., 1959. Use of dinitrosalicylic reagent for determination of reducing sugars. Anal. Chem., 31, 426-428.
23. MOIR, R. J., 1991. The role of microbes in digestion. In: Microbiology of Animals and Animal Products (J. B. WOOLCOCK, editor), pp. 29-59. Elsevier Science.
24. NAKAJIMA, N., ISHIHARA, K. and MATSUURA, Y., 2002. Dietary-fiber- degrading enzymes from a human intestinal Clostridium and their application to oligosaccharide production from nonstarchy polysaccharides using immobilized cells. Appl. Microbiol. Biotechnol., 59, 182-189.
25. POXTON, I. R., BROWN, R., SAWYER, A. and FERGUSON, A., 1997. Mucosa-associated bacterial flora of the human colon. J. Med. Microbiol., 46, 85-91.
26. SALANITRO, J. P., BLAKE, I. G. and MUIRHEAD, P. A., 1974a. Studies on the cecal microflora of commercial broiler chickens. Appl. Microbiol., 28, 439-447.
27. SALANITRO, J. P., FAIRCHILDS, I. G. and ZGORNICKI, Y. D., 1974b. Isolation, culture characteristics, and identification of anaerobic bacteria from the chicken cecum. Appl. Environ. Microbiol., 27, 678-687.
28. SALANITRO, J. P., BLAKE, I. G., MUIRHEAD, P. A., MAGILO, M. and GOODMAN, J. R., 1978. Bacteria isolated from the duodenum, ileum, and caecum of young chicks. Appl. Environ. Microbiol., 35, 782-790.
29. SALYERS, A. A., PALMER, J. K. and WILKINS, T. D., 1977a. Laminarinase (beta-glucanase) activity in Bacteroides from the human colon. Appl. Environ. Microbiol., 33, 1118-1124.
30. SALYERS, A. A., VERCELLOTTI, J. R, WEST, S. E. H. and WILKINS, T. D., 1977b. Fermentation of mucin and plant polysaccharides by strains of Bacteroides from the human colon. Appl. Environ. Microbiol., 33, 319-322.
31. SCHIMMING, S., SCHWARZ, W. H. and STAUDENBAUER, W. L., 1991. Properties of a thermoactive beta-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli. Biochem. Biophys. Res. Comm., 31, 447-452.
32. SCHIMMING, S., SCHWARZ, W. H. and STAUDENBAUER, W. L., 1992. Structure of the Clostridium thermocellum gene licB and the encoded beta-1,3-1,4-glucanase. A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases. Eur. J. Biochem., 204, 13-19.
33. SCHWARZ, W. H., SCHIMMING, S. and STAUDENBAUER, W. L., 1985. Molecular cloning of Clostridium thermocellum genes involved in β-glucan degradation in bacteriophage Lambda. Biotechnol. Lett., 7, 859-864.
34. SCHWARZ, W. H., BRONNENMEIER, K., GRAEBNITZ, F. and STAUDENBAUER, W. L., 1987. Activity staining of cellulases in polyacrylamide gels containing mixed linkage β-glucans. Anal. Biochem., 164, 72-77.
35. SIROTEK, K., MAROUNEK, M., RADA, V. and BENDA, V., 2001. Isolation and characterization of rabbit caecal pectinolytic bacteria. Folia Microbiol (Praha), 46, 79-82.
36. TARAS, D., SIMMERING, R., COLLINS, M. D., LAWSON, P. A. and BLAUT, M., 2002. Reclassification of Eubacterium formicigenerans Holdeman and Moore 1974 as Dorea formicigenerans gen. nov., comb, nov., and description of Dorea longicatena sp. nov., isolated from human faeces. Int. J. Syst. Evol. Micr., 52, 423-428.
37. TEATHER, R. M. and ERFLE, J. D., 1990. DNA sequence of a Fibrobacter succinogenes mixed-linkage β-glucanase (1,3-1,4-β-D-glucan-4- glucanohydrolase) gene. J. Bacteriol., 172, 3837-3841.
38. TEATHER, R. M. and WOOD, P. J., 1982. Use of congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol., 43, 777-780.
39. VAHJEN, W., GLÄSER, K., FROECK, M. and SIMON, O., 1997. Non starch polysaccharide hydrolyzing enzymes as feed additives: detection of enzyme activities and problems encountered with quantitative determination in complex samples. Arch. Tierernähr., 50, 331-345.
40. VALENTINE, P. J. and SALYERS, A. A., 1992. Analysis of proteins associated with growth of Bacteroides ovatus on the branched galactomannan guar gum. Appl. Environ. Microbiol., 58, 1534-1540
41. WHITEHEAD, T. R., 1995. Nucleotide sequences of xylan-inducible xylanase and xylosidase/arabinosidase genes from Bacteroides ovatus V975. Biochim. Biophys. Acta, 1244, 239-241.
42. WINTER, J., POPOFF, M. R., GRIMONT, P. and BOKKENHEUSER, V. D., 1991. Clostridium orbiscindens sp. nov., a human intestinal bacterium capable for cleaving the flavonoid C-ring. Int. J. Syst. Bacteriol., 41, 355-357.
43. WOLF, M., GEZCI, A., SIMON, O. and BORRISS, R., 1995. Genes encoding xylan and β-glucan hydrolyzing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase. Microbiol., 141, 281-290.