-
1
-
-
4344650085
-
Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins
-
Nordlund H.R., Laitinen O.H., Hytönen V.P., Uotila S.T., Porkka E., and Kulomaa M.S. Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins. J. Biol. Chem. 279 (2004) 36715-36719
-
(2004)
J. Biol. Chem.
, vol.279
, pp. 36715-36719
-
-
Nordlund, H.R.1
Laitinen, O.H.2
Hytönen, V.P.3
Uotila, S.T.4
Porkka, E.5
Kulomaa, M.S.6
-
2
-
-
27544465783
-
Dual-affinity avidin molecules
-
Hytönen V.P., Nordlund H.R., Hörhä J., Nyholm T.K., Hyre D.E., Kulomaa T., et al. Dual-affinity avidin molecules. Proteins: Struct. Funct. Bioinf. 61 (2005) 597-607
-
(2005)
Proteins: Struct. Funct. Bioinf.
, vol.61
, pp. 597-607
-
-
Hytönen, V.P.1
Nordlund, H.R.2
Hörhä, J.3
Nyholm, T.K.4
Hyre, D.E.5
Kulomaa, T.6
-
4
-
-
0037462730
-
Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces
-
Nordlund H.R., Laitinen O.H., Uotila S.T., Nyholm T., Hytönen V.P., Slotte J.P., and Kulomaa M.S. Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces. J. Biol. Chem. 278 (2003) 2479-2483
-
(2003)
J. Biol. Chem.
, vol.278
, pp. 2479-2483
-
-
Nordlund, H.R.1
Laitinen, O.H.2
Uotila, S.T.3
Nyholm, T.4
Hytönen, V.P.5
Slotte, J.P.6
Kulomaa, M.S.7
-
5
-
-
0029797773
-
Streptavidins with intersubunit crosslinks have enhanced stability
-
Reznik G.O., Vajda S., Smith C.L., Cantor C.R., and Sano T. Streptavidins with intersubunit crosslinks have enhanced stability. Nature Biotechnol. 14 (1996) 1007-1011
-
(1996)
Nature Biotechnol.
, vol.14
, pp. 1007-1011
-
-
Reznik, G.O.1
Vajda, S.2
Smith, C.L.3
Cantor, C.R.4
Sano, T.5
-
6
-
-
0037423398
-
Rational design of an active avidin monomer
-
Laitinen O.H., Nordlund H.R., Hytönen V.P., Uotila S.T., Marttila A.T., Savolainen J., et al. Rational design of an active avidin monomer. J. Biol. Chem. 278 (2003) 4010-4014
-
(2003)
J. Biol. Chem.
, vol.278
, pp. 4010-4014
-
-
Laitinen, O.H.1
Nordlund, H.R.2
Hytönen, V.P.3
Uotila, S.T.4
Marttila, A.T.5
Savolainen, J.6
-
7
-
-
0036415244
-
Design, production, and characterization of a monomeric streptavidin and its application for affinity purification of biotinylated proteins
-
Qureshi M.H., and Wong S.L. Design, production, and characterization of a monomeric streptavidin and its application for affinity purification of biotinylated proteins. Protein Expr. Purif. 25 (2002) 409-415
-
(2002)
Protein Expr. Purif.
, vol.25
, pp. 409-415
-
-
Qureshi, M.H.1
Wong, S.L.2
-
8
-
-
20744437590
-
Engineering soluble monomeric streptavidin with reversible biotin binding capability
-
Wu S.C., and Wong S.L. Engineering soluble monomeric streptavidin with reversible biotin binding capability. J. Biol. Chem. 280 (2005) 23225-23231
-
(2005)
J. Biol. Chem.
, vol.280
, pp. 23225-23231
-
-
Wu, S.C.1
Wong, S.L.2
-
9
-
-
0032704893
-
Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain
-
Laitinen O.H., Airenne K.J., Marttila A.T., Kulik T., Porkka E., Bayer E.A., et al. Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain. FEBS Letters 461 (1999) 52-58
-
(1999)
FEBS Letters
, vol.461
, pp. 52-58
-
-
Laitinen, O.H.1
Airenne, K.J.2
Marttila, A.T.3
Kulik, T.4
Porkka, E.5
Bayer, E.A.6
-
10
-
-
0347627526
-
Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding
-
Nordlund H.R., Hytönen V.P., Laitinen O.H., Uotila S.T., Niskanen E.A., Savolainen J., et al. Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding. FEBS Letters 555 (2003) 449-454
-
(2003)
FEBS Letters
, vol.555
, pp. 449-454
-
-
Nordlund, H.R.1
Hytönen, V.P.2
Laitinen, O.H.3
Uotila, S.T.4
Niskanen, E.A.5
Savolainen, J.6
-
11
-
-
0346525131
-
Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences
-
Marttila A.T., Hytönen V.P., Laitinen O.H., Bayer E.A., Wilchek M., and Kulomaa M.S. Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences. Biochem. J. 369 (2003) 249-254
-
(2003)
Biochem. J.
, vol.369
, pp. 249-254
-
-
Marttila, A.T.1
Hytönen, V.P.2
Laitinen, O.H.3
Bayer, E.A.4
Wilchek, M.5
Kulomaa, M.S.6
-
12
-
-
0029793803
-
Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin
-
Bayer E.A., Ehrlich-Rogozinski S., and Wilchek M. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin. Electrophoresis 17 (1996) 1319-1324
-
(1996)
Electrophoresis
, vol.17
, pp. 1319-1324
-
-
Bayer, E.A.1
Ehrlich-Rogozinski, S.2
Wilchek, M.3
-
13
-
-
10644245917
-
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
-
Hytönen V.P., Laitinen O.H., Airenne T.T., Kidron H., Meltola N.J., Porkka E., et al. Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli. Biochem. J. 384 (2004) 385-390
-
(2004)
Biochem. J.
, vol.384
, pp. 385-390
-
-
Hytönen, V.P.1
Laitinen, O.H.2
Airenne, T.T.3
Kidron, H.4
Meltola, N.J.5
Porkka, E.6
-
14
-
-
20844452424
-
Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein
-
Aslan F.M., Yu Y., Mohr S.C., and Cantor C.R. Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein. Proc. Natl Acad. Sci. USA 102 (2005) 8507-8512
-
(2005)
Proc. Natl Acad. Sci. USA
, vol.102
, pp. 8507-8512
-
-
Aslan, F.M.1
Yu, Y.2
Mohr, S.C.3
Cantor, C.R.4
-
15
-
-
29644436931
-
Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins
-
Nordlund H.R., Hytönen V.P., Hörhä J., Määttä J.A., White D.J., Halling K., et al. Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins. Biochem. J. 392 (2005) 485-491
-
(2005)
Biochem. J.
, vol.392
, pp. 485-491
-
-
Nordlund, H.R.1
Hytönen, V.P.2
Hörhä, J.3
Määttä, J.A.4
White, D.J.5
Halling, K.6
-
17
-
-
22844445360
-
High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability
-
Eisenberg-Domovich Y., Hytönen V.P., Wilchek M., Bayer E.A., Kulomaa M.S., and Livnah O. High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability. Acta Crystallog. sect. D 61 (2005) 528-538
-
(2005)
Acta Crystallog. sect. D
, vol.61
, pp. 528-538
-
-
Eisenberg-Domovich, Y.1
Hytönen, V.P.2
Wilchek, M.3
Bayer, E.A.4
Kulomaa, M.S.5
Livnah, O.6
-
18
-
-
0024588901
-
Structural origins of high-affinity biotin binding to streptavidin
-
Weber P.C., Ohlendorf D.H., Wendoloski J.J., and Salemme F.R. Structural origins of high-affinity biotin binding to streptavidin. Science 243 (1989) 85-88
-
(1989)
Science
, vol.243
, pp. 85-88
-
-
Weber, P.C.1
Ohlendorf, D.H.2
Wendoloski, J.J.3
Salemme, F.R.4
-
19
-
-
0027214259
-
Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7 Å resolution
-
Pugliese L., Coda A., Malcovati M., and Bolognesi M. Three-dimensional structure of the tetragonal crystal form of egg-white avidin in its functional complex with biotin at 2.7 Å resolution. J. Mol. Biol. 231 (1993) 698-710
-
(1993)
J. Mol. Biol.
, vol.231
, pp. 698-710
-
-
Pugliese, L.1
Coda, A.2
Malcovati, M.3
Bolognesi, M.4
-
20
-
-
0036566145
-
Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin
-
Laitinen O.H., Hytönen V.P., Ahlroth M.K., Pentikäinen O.T., Gallagher C., Nordlund H.R., et al. Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin. Biochem. J. 363 (2002) 609-617
-
(2002)
Biochem. J.
, vol.363
, pp. 609-617
-
-
Laitinen, O.H.1
Hytönen, V.P.2
Ahlroth, M.K.3
Pentikäinen, O.T.4
Gallagher, C.5
Nordlund, H.R.6
-
21
-
-
25444490147
-
Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family
-
Niskanen E.A., Hytönen V.P., Grapputo A., Nordlund H.R., Kulomaa M.S., and Laitinen O.H. Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family. BMC Genomics 6 (2005) 41
-
(2005)
BMC Genomics
, vol.6
, pp. 41
-
-
Niskanen, E.A.1
Hytönen, V.P.2
Grapputo, A.3
Nordlund, H.R.4
Kulomaa, M.S.5
Laitinen, O.H.6
-
22
-
-
17144400352
-
Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum
-
Nordlund H.R., Hytönen V.P., Laitinen O.H., and Kulomaa M.S. Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum. J. Biol. Chem. 280 (2005) 13250-13255
-
(2005)
J. Biol. Chem.
, vol.280
, pp. 13250-13255
-
-
Nordlund, H.R.1
Hytönen, V.P.2
Laitinen, O.H.3
Kulomaa, M.S.4
-
23
-
-
0025325983
-
The "megaprimer" method of site-directed mutagenesis
-
Sarkar G., and Sommer S.S. The "megaprimer" method of site-directed mutagenesis. Biotechniques 8 (1990) 404-407
-
(1990)
Biotechniques
, vol.8
, pp. 404-407
-
-
Sarkar, G.1
Sommer, S.S.2
-
24
-
-
0031078812
-
Production of biologically active recombinant avidin in baculovirus-infected insect cells
-
Airenne K.J., Oker-Blom C., Marjomäki V.S., Bayer E.A., Wilchek M., and Kulomaa M.S. Production of biologically active recombinant avidin in baculovirus-infected insect cells. Protein Expr. Purif. 9 (1997) 100-108
-
(1997)
Protein Expr. Purif.
, vol.9
, pp. 100-108
-
-
Airenne, K.J.1
Oker-Blom, C.2
Marjomäki, V.S.3
Bayer, E.A.4
Wilchek, M.5
Kulomaa, M.S.6
-
25
-
-
0027879008
-
Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants
-
Kabsch W. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J. Appl. Crystallog. 26 (1993) 795-800
-
(1993)
J. Appl. Crystallog.
, vol.26
, pp. 795-800
-
-
Kabsch, W.1
-
26
-
-
84920325457
-
Amore-An automated package for molecular replacement
-
Navaza J. Amore-An automated package for molecular replacement. Acta Crystallog. sect. A 50 (1994) 157-163
-
(1994)
Acta Crystallog. sect. A
, vol.50
, pp. 157-163
-
-
Navaza, J.1
-
27
-
-
0028103275
-
The CCP4 Suite: programs for protein crystallography
-
Collaborative Computational Project, number 4
-
Collaborative Computational Project, number 4. The CCP4 Suite: programs for protein crystallography. Acta Crystallog. sect. D 50 (1994) 760-763
-
(1994)
Acta Crystallog. sect. D
, vol.50
, pp. 760-763
-
-
-
28
-
-
27644560153
-
Avidin related protein 2 shows unique structural and functional features among the avidin protein family
-
Hytönen V.P., Määttä J.A., Kidron H., Halling K.K., Hörhä J., Kulomaa T., et al. Avidin related protein 2 shows unique structural and functional features among the avidin protein family. BMC Biotechnol. 5 (2005) 28
-
(2005)
BMC Biotechnol.
, vol.5
, pp. 28
-
-
Hytönen, V.P.1
Määttä, J.A.2
Kidron, H.3
Halling, K.K.4
Hörhä, J.5
Kulomaa, T.6
-
29
-
-
0032964481
-
Automated protein model building combined with iterative structure refinement
-
Perrakis A., Morris R., and Lamzin V.S. Automated protein model building combined with iterative structure refinement. Nature Struct. Biol. 6 (1999) 458-463
-
(1999)
Nature Struct. Biol.
, vol.6
, pp. 458-463
-
-
Perrakis, A.1
Morris, R.2
Lamzin, V.S.3
-
30
-
-
0030924992
-
Refinement of macromolecular structures by the maximum-likelihood method
-
Murshudov G.N., Vagin A.A., and Dodson E.J. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallog. sect. D 53 (1997) 240-255
-
(1997)
Acta Crystallog. sect. D
, vol.53
, pp. 240-255
-
-
Murshudov, G.N.1
Vagin, A.A.2
Dodson, E.J.3
-
31
-
-
84889120137
-
Improved methods for building protein models in electron density maps and the location of errors in these models
-
Jones T.A., Zou J.Y., Cowan S.W., and Kjeldgaard. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallog. sect. A 47 (1991) 110-119
-
(1991)
Acta Crystallog. sect. A
, vol.47
, pp. 110-119
-
-
Jones, T.A.1
Zou, J.Y.2
Cowan, S.W.3
Kjeldgaard4
-
34
-
-
0025398721
-
WHAT IF: a molecular modeling and drug design program
-
Vriend G. WHAT IF: a molecular modeling and drug design program. J. Mol. Graph. 8 (1990) 52-56
-
(1990)
J. Mol. Graph.
, vol.8
, pp. 52-56
-
-
Vriend, G.1
|