CPD staining: An effective technique for detection of NORs and other GC-rich chromosomal regions in plants

Biotechnic and Histochemistry

Volumn 81, Issue 1, 2006, Pages 13-21

  She, C W ^a,b   Liu, J Y ^a,c   Song, Yunchun C ^a  

^a WUHAN UNIVERSITY   (China)

^b HUAIHUA UNIVERSITY   (China)

^c HENAN UNIVERSITY   (China)

Author keywords

Chromomycin A3; DAPI staining; FISH; GC rich region; NOR; Plant chromosomes; Propidium iodide

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; CHROMOMYCIN A3; CYTOSINE; DNA 45S; GUANINE; PLANT DNA; PROPIDIUM IODIDE; RIBOSOME DNA; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; BARLEY; BEAN; CABBAGE; CHROMOSOME BANDING PATTERN; CHROMOSOME NOR; CONTROLLED STUDY; DNA PROBE; FLUORESCENCE IN SITU HYBRIDIZATION; FLUOROCHROME STAINING; GARLIC; GC RICH SEQUENCE; MAIZE; MITOSIS; NONHUMAN; ONION; PEA; PLANT CHROMOSOME; RICE; RYE; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS; SETARIA; SORGHUM; SPECIES DIFFERENCE; TOMATO;

CHROMOSOME BANDING; CHROMOSOMES; FLUORESCENT DYES; GC RICH SEQUENCE; INDOLES; NUCLEOLUS ORGANIZER REGION; PLANTS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING;

AEGILOPS TAUSCHII; ALLIUM CEPA; ALLIUM SATIVUM; ARABIDOPSIS THALIANA; BRASSICA OLERACEA VAR. CAPITATA; HORDEUM VULGARE; LYCOPERSICON ESCULENTUM; ORYZA; ORYZA OFFICINALIS; PISUM SATIVUM; PSOPHOCARPUS TETRAGONOLOBUS; SATIVUM; SECALE CEREALE; SETARIA ITALICA; SORGHUM BICOLOR; VICIA FABA; ZEA MAYS;

EID: 33745094699     PISSN: 10520295     EISSN: 14737760     Source Type: Journal    
DOI: 10.1080/10520290600661414     Document Type: Article

References (32)

1. Andras, SC and Hartman, TPV and Alexander, J and Mcbride, R and Marshall, JA and Power, JB and Cocking, EC and Davey, MR. (2000) Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes Chromosome Res., 8, pp. 387-391.
2. Cerbah, M and Coulaud, J and Siljak-Yakovlev, S. (1998) rDNA organization and evolutionary relationships in the genus Hypochoeris (Asteraceae) J. Hered., 89, pp. 312-318.
3. Doudrick, RL and Heslop-Harrison, JS and Nelson, CD and Schmidt, T and Nance, WL and Schwarzacher, T. (1995) Karyotype of slash pine (Pinus elliottii var. elliottii) using patterns of fluorescence in situ hybridization and fluorochrome banding J. Hered., 86, pp. 289-296.
4. Gerlach, WL and Bedbrook, J R. (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley Nucl. Acids Res., 7, pp. 1869-1885.
5. Hajdera, I and Siwinska, D and Hasterok, R and Maluszynska, J. (2003) Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii Theor. Appl. Genet., 107, pp. 988-996.
6. Hanson, RE and Zwick, MS and Choi, S and Islam-Faridi, MN and Mcknight, T and Wing, RA and Price, HJ and Stelly, DM. (1995) Fluorescent in situ hybridization of a bacterial artificial chromosome Genome, 38, pp. 646-651.
7. Hizume, M. (1992) Exact location of rRNA genes in Vicia faba chromosomes Cytologia, 57, pp. 471-475.
8. Hoshi, Y and Plader, W and Malepszy, S. (1999) Physical mapping of 45S rRNA gene loci in the cucumber (Cucumis sativus L.) using fluorescence in situ hybridization Caryologia, 52, pp. 49-57.
9. Hudakova, S and Michalek, W and Presting, GG and ten Hoopen, R and dos Santos, K and Jasencakova, Z and Schubert, I. (2001) Sequence organization of barley centromeres Nucl. Acids Res., 29, pp. 5029-5035.
10. Ingle, J and Timmis, JN and Sinclair, J. (1975) The relationship between satellite deoxyribonucleic acid, ribosomal ribonucleic acid gene redundancy, and genome size in plants Plant Physiol., 55, pp. 496-501.
11. Ji, Y and Raska, DA and McKnight, TD and Islam-Faridi, MN and Crane, CF and Zwick, MS and Hanson, RE and Price, HJ and Stelly, DM. (1997) Use of meiotic FISH for identification of a new monosome in Gossypium hirsutum L Genome, 40, pp. 34-40.
12. Kato, S and Omido, N and Fukui, F. (2003) Development of a quantitative pachytene chromosome map in Oryza sativum by imaging methods Genes Genet. Syst., 78, pp. 155-161.
13. Kim, ES and Punina, EO and Rodionov, AV. (2002) Chromosome CPD (PI/ DAPI)- and CMA/DAPI-banding patterns in Allium cepa L Russian J. Genet., 38, pp. 392-398.
14. Kiss, T and Kis, M and Abel, S and Solymosy, F. (1988) Nucleotide sequence of the 17-25S spacer region from tomato rDNA Nucl. Acids Res., 16, pp. 7179.
15. Lim, KB and Wennekes, J and de Jong, JH and Jacobsen, E and van Tuyl, JM. (2001) Karyotype analysis of Lilium longiflorum and Lilium rubellum by chromosome banding and fluorescence in situ hybridization Genome, 44, pp. 911-918.
16. Macgregor, HC and Kezer, J. (1971) The chromosomal localization of a heavy satellite DNA in the testis of Plethodon c. cinereus Chromosoma, 33, pp. 167-182.
17. Miranda, M and Ikeda, F and Endo, T and Moriguchi, T and Omura, M. (1997a) Comparative analysis on the distribution of heterochromatin in Citrus, Poncirus and Fortunella chromosomes Chromosome Res., 5, pp. 86-92.
18. Miranda, M and Ikeda, F and Endo, T and Moriguchi, T and Omura, M. (1997b) rDNA sites and heterochromatin in Meiwa kumquat (Fortunella crassifolia Swing.) chromosomes revealed by FISH and CMA/DAPI staining Caryologia, 50, pp. 333-340.
19. Panzera, F and Giménez-Abián, MI and López-Sáez, JF and Giménez-Martín, G and Cuadrado Shaw, PJ and Beven, AF and Cánovas, JL and De la Torre, C. (1996) Nucleolar organizer expression in Allium cepa L. chromosomes Chromosoma, 105, pp. 12-19.
20. Peterson, DG and Lapitan, NLV and Stack, SM. (1999) Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH) Genetics, 152, pp. 427-439.
21. Pich, U and Fuchs, J and Schubert, I. (1996) How do Alliaceae stabilize their chromosome ends in the absence of TTTAGGG sequences? Chromosome Res., 4, pp. 207-213.
22. Saccone, S and De Sario, A and Della Valle, G and Bernardi, G. (1992) The highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes Proc. Natl. Acad. Sci. USA., 89, pp. 4913-4917.
23. Schubert, I and Fransz, PF and Fuchs, J and de Jong, JH. (2001) Chromosome painting in plants Methods Cell Sci., 23, pp. 57-69.
24. Schweizer, D. (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI Chromosoma, 58, pp. 307-324.
25. Siljak-Yakovlev, S and Cerbah, M and Coulaud, J and Stoian, V and Brown, SC and Zoldos, V and Jelenic, S and Papes, D. (2002) Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies Theor. Appl. Genet., 104, pp. 505-512.
26. Siljak-Yakovlev, S and Peccenini, S and Muratovic, E and Zoldos, V and Robin, O and Vallés, J. (2003) Chromosomal differentiation and genome size in three European mountain Lilium species Plant Syst. Evol., 236, pp. 165-173.
27. Song, YC and Liu, LH and Ding, Y and Tian, XB and Yao, Q and Meng, L and He, CR and Xu, MS. (1994) Comparisons of G-Banding patterns in six species of the Poaceae Hereditas, 121, pp. 31-38.
28. Sumner, AT.(1990) Chromosome banding. London: Unwin Hyman.
29. Takaiwa, F and Kikuchi, S and Oono, K. (1990) The complete nucleotide sequence of the intergenic spacer between 25S and 17S rDNAs in rice Plant Mol. Biol., 15, pp. 933-935.
30. Vallés Xirau, J and Siljak-Yakovlev, S. (1997) Cytogenetic studies in the genus Artemisia L. (Asteraceae): Fluorochrome-banded karyotypes of five taxa, including the Iberian endemic species Artemisia barrelieri Besser Can. J. Bot., 75, pp. 595-606.
31. Yasmineh, WG and Yunis, JJ. (1971) Satellite DNA in calf heterochromatin Exp. Cell Res., 64, pp. 41-48.
32. Zoldos, V and Papes, D and Cerbah, M and Panaud, O and Besendorfer, V and Siljak-Yakovlev, S. (1999) Molecular-cytogenetic studies of ribosomal genes and heterochromatin reveal conserved genome organization among eleven Quercus species Theor. Appl. Genet., 99, pp. 969-977.