Two pathways of electron transport to nitrogenase in Rhodospirillum rubrum: The major pathway is dependent on the fix gene products

FEMS Microbiology Letters

Volumn 260, Issue 1, 2006, Pages 30-35

  Edgren, Tomas ^a   Nordlund, Stefan ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

Electron transport; Fix genes; Nitrogenase; Rhodospirillum rubrum

Indexed keywords

BACTERIAL ENZYME; DINITROGENASE REDUCTASE; FERREDOXIN; GENE PRODUCT; NITROGENASE; PYRUVATE SYNTHASE;

ANAEROBIC METABOLISM; ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; CONTROLLED STUDY; ELECTRON TRANSPORT; ENZYME KINETICS; GENETIC REGULATION; HETEROTROPHY; MODEL; MUTANT; NIFJ GENE; NONHUMAN; PHOTOTROPHIC BACTERIUM; PRIORITY JOURNAL; PROTON MOTIVE FORCE; RHODOSPIRILLUM RUBRUM;

BACTERIAL PROTEINS; ELECTRON TRANSPORT; NITROGENASE; RHODOSPIRILLUM RUBRUM;

PHOTOBACTERIA; RHODOSPIRILLUM RUBRUM;

EID: 33745023377     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00297.x     Document Type: Article

References (30)

1. Arigoni F Kaminski PA Hennecke H Elmerich C 1991 Nucleotide sequence of the fixABC region of Azorhizobium caulinodans ORS571: similarity of the fixB product with eukaryotic flavoproteins, characterization of fixX, and identification of nifW. Mol Gen Genet 225 : 514 520.
2. Bauer CC Scappino L Haselkorn R 1993 Growth of the cyanobacterium Anabaena on molecular nitrogen: nifJ is required when iron is limited. Proc Natl Acad Sci USA 90 : 8812 8816.
3. Earl CD Ronson CW Ausubel FM 1987 Genetic and structural analysis of the Rhizobium meliloti fixA, fixB, fixC, and fixX genes. J Bacteriol 169 : 1127 1136.
4. Edgren T Nordlund S 2004 The fixABCX genes in Rhodospirillum rubrum encode a putative membrane complex participating in electron transfer to nitrogenase. J Bacteriol 186 : 2052 2060.
5. Edgren T Nordlund S 2005 Electron transport to nitrogenase in Rhodospirillum rubrum: identification of a new fdxN gene encoding the primary electron donor to nitrogenase. FEMS Microbiol Lett 245 : 345 351.
6. Goodman SI Axtell KM Bindoff LA Beard SE Gill RE Frerman FE 1994 Molecular cloning and expression of a cDNA encoding human electron transfer flavoprotein-ubiquinone oxidoreductase. Eur J Biochem 219 : 277 286.
7. Gronger P Manian SS Reilander H O'Connell M Priefer UB Pühler A 1987 Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Res 15 : 31 49.
8. Gubler M Zurcher T Hennecke H 1989 The Bradyrhizobium japonicum fixBCX operon: identification of fixX and of a 5′ mRNA region affecting the level of the fixBCX transcript. Mol Microbiol 3 : 141 148.
9. Hanahan D 1985 Techniques for transformation of. E. coli, Vol. 1 Clover M, ed), pp. 109 135. IRL Press, Oxford.
10. Jouanneau Y Meyer C Naud I Klipp W 1995 Characterization of an fdxN mutant of Rhodobacter capsulatus indicates that ferredoxin I serves as electron donor to nitrogenase. Biochim Biophys Acta 1232 : 33 42.
11. Kaminski PA Norel F Desnoues N Kush A Salzano G Elmerich C 1988 Characterization of the fixABC region of Azorhizobium caulinodans ORS571 and identification of a new nitrogen fixation gene. Mol Gen Genet 214 : 496 502.
12. Lee S Reth A Meletzus D Sevilla M Kennedy C 2000 Characterization of a major cluster of nif, fix, and associated genes in a sugarcane endophyte, Acetobacter diazotrophicus. J Bacteriol 182 : 7088 7091.
13. Lindblad A Nordlund S 1997 Electron transport to nitrogenase in Rhodospirillum rubrum: role of energization of the chromatophore membrane. Photosynth Res 53 : 23 28.
14. Lindblad A Jansson J Brostedt E Johansson M Hellman U Nordlund S 1996 Identification and sequence of a nifJ-like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation. Mol Microbiol 20 : 559 568.
15. Lowry OH Rosebrough NJ Farr AL Randall RJ 1951 Protein measurement with the folin phenol reagent. J Biol Chem 193 : 265 275.
16. Ludden PW 1991 Energetics and sources of energy for biological nitrogen fixation. Curr Top Bioenerg 16 : 369 390.
17. Ludden PW Burris RH 1981 In vivo and in vitro studies on ATP and electron donors to nitrogenase in Rhodospirillum rubrum. Arch Microbiol 130 : 155 158.
18. Mather MW McReynolds LM Yu CA 1995 An enhanced broad-host-range vector for gram-negative bacteria: avoiding tetracycline phototoxicity during the growth of photosynthetic bacteria. Gene 156 : 85 88.
19. Ormerod JG Ormerod KS Gest H 1961 Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys 94 : 449 463.
20. Paul TD Ludden PW 1984 Adenine nucleotide levels in Rhodospirillum rubrum during switch-off of whole-cell nitrogenase activity. Biochem J 224 : 961 969.
21. Schweizer HP 1993 Small broad-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. BioTechniques 15 : 831 834.
22. Scott JD Ludwig RA 2004 Azorhizobium caulinodans electron-transferring flavoprotein N electrochemically couples pyruvate dehydrogenase complex activity to N2 fixation. Microbiology 150 : 117 126.
23. Shah VK Stacey G Brill WJ 1983 Electron transport to nitrogenase. Purification and characterization of pyruvate: flavodoxin oxidoreductase. The nifJ gene product. J Biol Chem 258 : 12064 12068.
24. Simon R Priefer U Pühler AA 1983 A broad host range mobilisation system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1 : 784 791.
25. Sperotto RA Gross J Vedoy C Passaglia LMP Schrank IS 2004 The electron transfer flavoprotein fixABCX gene products from Azospirillum brasilense show a NifA-dependent promoter regulation. Curr Microbiol 49 : 267 273.
26. Tsai MH Saier MH Jr. 1995 Phylogenetic characterization of the ubiquitous electron transfer flavoprotein families ETF-alpha and ETF-beta. Res Microbiol 146 : 397 404.
27. Weidenhaupt M Rossi P Beck C Fischer HM Hennecke H 1996 Bradyrhizobium japonicum possesses two discrete sets of electron transfer flavoprotein genes: fixA, fixB and etfS, etfL. Arch Microbiol 165 : 169 178.
28. Yakunin AF Hallenbeck PC 1998 Purification and characterization of pyruvate oxidoreductase from the photosynthetic bacterium Rhodobacter capsulatus. Biochim Biophys Acta 1409 : 39 49.
29. Yoch DC Arnon DI 1975 Comparison of two ferredoxins from Rhodospirillum rubrum as electron carriers for the native nitrogenase. J Bacteriol 121 : 743 745.
30. Yoch DC Arnon DI Sweeney WV 1975 Characterization of two soluble ferredoxins as distinct from bound iron-sulfur proteins in the photosynthetic bacterium Rhodospirillum rubrum. J Biol Chem 250 : 8330 8336.