Identification and sequence of a nifJ-like gene in Rhodospirillum rubrum: Partial characterization of a mutant unaffected in nitrogen fixation

Molecular Microbiology

Volumn 20, Issue 3, 1996, Pages 559-568

  Lindblad, Anders ^a   Jansson, Janet ^a   Brostedt, Erica ^c   Johansson, Magnus ^a   Hellman, Ulf ^b   Nordlund, Stefan ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b LUDWIG INSTITUTE FOR CANCER RESEARCH   (Sweden)

^c MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; DNA SEQUENCE; KLEBSIELLA PNEUMONIAE; NITROGEN FIXATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RHODOSPIRILLUM RUBRUM;

EID: 0029878945     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.5311054.x     Document Type: Article

References (38)

1. Arnold, W., Rump, A., Klipp, W., Priefer, U.B., and Pühler, A. (1988) Nucleotide sequence of a 24206-base pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae. J Mol Biol 203: 715-738.
2. Ausubel, F.M., Brebt, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1991) Current Protocols in Molecular Biology. Vol. 1. New York: Greene Publishing Associates and Wiley-Interscience.
3. Bauer, C.C., Scappino, L., and Haselkorn, R. (1993) Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required only when iron is limited. Proc Natl Acad Sci USA 90: 8812-8816.
4. Bogusz, D., Houmard, J., and Aubert, J.-P. (1981) Electron transport to nitrogenase in Klebsiella pneumoniae. Eur J Biochem 120: 421-426.
5. Brostedt, E., and Nordlund, S. (1991) Purification and partial characterization of a pyruvate oxidoreductase from the photosynthetic bacterium Rhodospirillum rubrum grown under nitrogen fixing conditions. Biochem J 279: 155-158.
6. Buchanan, B.B., Evans, M.C.W., and Arnon, D.I. (1967) Ferredoxin dependent carbon assimilation in Rhodospirillum rubrum. Arch Microbiol 59: 32-40.
7. Cusanovich, M.A., and Edmondson, D.E. (1971) The isolation and characterization of Rhodospirillum rubrum flavodoxin. Biochem Biophys Res Commun 45: 327-336.
8. Deistung, J., and Thorneley, R.N.F. (1986) Electron transfer to nitrogenase. Biochem J 239: 69-75.
9. Deistung, J., Cannon, F.C., Cannon, M.C., Hill, S., and Thorneley, R.N.F. (1985) Electron transfer to nitrogenase in Klebsiella pneumoniae. Biochem J 231: 743-753.
10. Falk, G., Hampe, A., and Walker, J.E. (1985) Nucleotide sequence of the Rhodospirillum rubrum atp operon. Biochem J 228: 391-407.
11. Genetics Computer Group (1991) Program Manual for the GCG Package version 7, April 1991, 575 Science Drive, Madison, Wi 53711, USA.
12. Gorrell, T.E., and Uffen, R.L. (1978) Reduction of nicotinamide adenine dinucleotide by pyruvate:lipoate oxidoreductase in anaerobic, dark-grown Rhodospirillum rubrum Mutant C. J Bacteriol 134: 830-836.
13. Hallenbeck, P.C., Jouanneau, Y., and Vignais, P.M. (1982) Purification and molecular properties of a soluble ferredoxin from Rhodopseudomonas capsulata. Biochim Biophys Acta 681: 168-176.
14. Hill, S., and Kavanagh, E.P. (1980) Roles of nifF and nifJ gene products in electron transport to nitrogenase in Klebsiella pneumoniae. J Bacteriol 141: 470-475.
15. Klipp, W. (1990) Organization and regulation of nitrogen fixation genes in Rhodobacter capsulatus. In Nitrogen Fixation: Achievements and Objectives. Gresshoff, P.G.M., Roth, L.E., Stacey, G., and, Newton, W.E. (eds). New York: Chapman and Hall, pp. 467-474.
16. Kranz, R.G., and Foster-Hartnett, D. (1990) Transcriptional regulatory cascade of nitrogen-fixation genes anoxygenic photosynthetic bacteria: oxygen- and nitrogen-responsive factors. Mol Microbiol 4: 1793-1800.
17. Kreutzer, R., Singh, M., and Klingmuller, W. (1989) Identification and characterization of the nifH and nifJ promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerans 333. Gene 78: 101-109.
18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
19. Ludden, P.W., and Burris, R.H. (1981) In vivo and in vitro studies on ATP and electron donors to nitrogenase in Rhodospirillum rubrum. Arch Microbiol 130: 155-158.
20. Lüderitz, R., and Klemme, J.-H. (1977) Isolierung und charakterisierung eines membrangebundenen pyruvatdehydrogenase-komplexes aus dem phototrophen bakterium Rhodospirillum rubrum. A Naturforsch 32: 351-361.
21. Mohamed, A., and Jansson, C. (1989) Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol 13: 693-700.
22. Morett, E., and Buck, M. (1989) In vivo studies on the interaction of RNA polymerase-S54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters: the role of NifA in the formation of an open promoter complex. J Mol Biol 210: 65-77.
23. Nieva-Gomez, D., Roberts, G.P., Klevickis, S., and Brill, W.J. (1980) Electron transport to nitrogenase in Klebsiella pneumoniae. Proc Natl Acad Sci USA 77: 2555-2558.
24. Nordlund, S., and Höglund, L. (1986) Studies on the adenylate and pyridine nucleotide pools during nitrogenase switch-off in Rhodospirillum rubrum. Plant Soil 90: 203-209.
25. Ormerod, J.G., Ormerod, K.S., and Gest, H. (1961) Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria: relationships with nitrogen metabolism. Arch Biochem Biophys 94: 449-463.
26. Roberts, G.P., MacNeil, T., MacNeil, D., and Brill, W.J. (1978) Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae. J Bacteriol 136: 267-279.
27. Rosenfeld, J., Capdevielle, J., Guillemot, J.C., and Ferrara, P. (1992) In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal Biochem 203: 173-179.
28. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
29. Schmitz, O., Kentemich, T., Zimmer, W., Hundeshagen, B., and Bothe, H. (1993) Identification of the nifJ gene encoding pyruvate:ferredoxin oxidoreductase in dinitrogen-fixing cyanobacteria. Arch Microbiol 160: 62-67.
30. Schultz, J.E., Gotto, J.W., Weaver, P.F., and Yoch, D.C. (1985) Regulation of nitrogen fixation in Rhodospirillum rubrum grown under dark, fermentative conditions. J Bacteriol 162: 1322-1324.
31. Shah, V.K., Stacey, G., and Brill, W.J. (1983) Electron transport to nitrogenase: purification and characterization of pyruvate:flavodoxin oxidoreductase, the nifJ gene product. J Biol Chem 258: 12064-12068.
32. Simon, R., Priefer, U., and Puhler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Biotechnology 1: 784-791.
33. Staden, R. (1982a) An interactive graphics program for comparing and aligning nucleic acid and amino sequences. Nucl Acids Res 10: 2951-2961.
34. Staden, R. (1982b) Automation of computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucl Acids Res 10: 4731-4751.
35. Veeger, C., Haaker, H., and Laane, C. (1981) Energy transduction and nitrogen fixation. In Current Perspectives in Nitrogen Fixation. Gibson, A.H., and Newton, W.E. (eds). Canberra: Australian Academy of Sciences, pp. 101-104.
36. Wahl, R.C., and Orme-Johnson, W.H. (1987) Clostridial pyruvate oxidoreductase and the pyruvate-oxidizing enzyme specific to nitrogen fixation in Klebsiella pneumoniae are similar enzymes. J Biol Chem 262: 10489-10496.
37. Yakunin, A.F., and Gogotov, I.N. (1983) Properties and regulation of synthesis of two ferredoxins from Rhodospseudomonas capsulata. Biochim Biophys Acta 725: 298-308.
38. Yoch, D.C., and Arnon, D.I. (1975) Comparison of two ferredoxins from Rhodospirillum rubrum as electron carriers for the native nitrogenase. J Bacteriol 121: 743-745.