Molecular cloning, expression and characterization of pyridoxamine-pyruvate aminotransferase

Biochemical Journal

Volumn 396, Issue 3, 2006, Pages 499-507

  Yoshikane, Yu ^a   Yokochi, Nana ^a   Ohnishi, Kouhei ^a   Hayashi, Hideyuki ^b   Yagi, Toshiharu ^a  

^a KOCHI UNIVERSITY   (Japan)

^b OSAKA MEDICAL COLLEGE   (Japan)

Author keywords

Active site structure; Mesorhizobium loti; Molecular cloning; Pyridoxal 5 phosphate independent aminotransferase; Pyridoxamine pyruvate aminotransferase (PPAT)

Indexed keywords

ABSORPTION; AMINO ACIDS; BACTERIA; CATALYSIS; CHROMOSOMES; CLONING; GENE TRANSFER; MOLECULAR STRUCTURE; NITROGEN FIXATION; VITAMINS;

ACTIVE-SITE STRUCTURE; MESORHIZOBIUM LOTI; MOLECULAR CLONING; PYRIDOXAL 5′-PHOSPHATE-INDEPENDENT AMINOTRANSFERASE; PYRIDOXAMINE-PYRUVATE AMINOTRANSFERASE (PPAT);

COENZYMES;

ALANINE; ASPARTATE AMINOTRANSFERASE; PYRIDOXAL 5 PHOSPHATE; PYRIDOXAMINE PYRUVATE AMINOTRANSFERASE; UNCLASSIFIED DRUG; AMINOTRANSFERASE; PYRIDOXAMINE-PYRUVATE TRANSAMINASE; RECOMBINANT PROTEIN;

ABSORPTION SPECTROSCOPY; AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; MESORHIZOBIUM; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; ALPHAPROTEOBACTERIA; AMINO ACID SEQUENCE; BINDING SITE; BIOSYNTHESIS; COMPARATIVE STUDY; ENZYMOLOGY; EVOLUTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PSEUDOMONAS; SITE DIRECTED MUTAGENESIS;

ARCHAEA; BACTERIA (MICROORGANISMS); MESORHIZOBIUM LOTI;

ALPHAPROTEOBACTERIA; AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; EVOLUTION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS; RECOMBINANT PROTEINS; TRANSAMINASES;

EID: 33745006425     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20060078     Document Type: Article

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