The expression, purification and crystallization of monomeric soluble human urokinase receptor

Progress in Biochemistry and Biophysics

Volumn 33, Issue 3, 2006, Pages 277-281

  Yuan, Cai ^a,c   Huai, Qing ^b   Bian, Chuan Bing ^a,c   Huang, Ming Dong ^a  

^a FUJIAN INSTITUTE OF RESEARCH ON THE STRUCTURE OF MATTER   (China)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Crystallization; Purification; S2 cells; Soluble human urokinase receptor

Indexed keywords

EID: 33744959745     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Ploug M. Structure-function relationships in the interaction between the urokinase-type plasminogen activator and its receptor. Curr Pharm Des, 2003, 9 (19): 1499-1528
2. Bdeir K, Kuo A, Mazar A, et al. A region in domain II of the urokinase receptor required for urokinase binding. J Biol Chem, 2000, 275 (37): 28532-28538
3. Tarui T, Mazar A P, Cines D B, et al. Urokinase-type plasminogen activator receptor (CD87) is a ligand for integrins and mediates cell-cell interaction. J Biol Chem, 2001, 276 (6): 3983-3990
4. Gardsvoll H, Werner F, Sondergaard L, et al. Characterization of low-glycosylated forms of soluble human urokinase receptor expressed in Drosophila Schneider 2 cells after deletion of glycosylation-sites. Protein Expr Purif, 2004, 34 (2): 284-295
5. Hansen L V, Gardsvoll H, Nielsen B S, et al. Structural analysis and tissue localization of human C4.4A: a protein homologue of the urokinase receptor. Biochem J, 2004, 380 (Pt 3): 845-857
6. 2-terminal domain of the cellular receptor for human urokinase-type plasminogen activator. A domain structure belonging to a novel superfamily of glycolipid-anchored membrane proteins. J Biol Chem, 1993, 268 (23): 17539-17546
7. Huang M, Mazar A P, Parry G, et al. Crystallization of soluble urokinase receptor (suPAR) in complex with urokinase amino-terminal fragment (1-143). Acta Crystallogr D Biol Crystallogr, 2005, 61 (Pt 6): 697-700
8. Huai Q, Mazar A, Kuo A, et al. Structure of human urokinase plasminogen activator in complex with its receptor. Science, 2006, 311 (5761): 656-659