Ultra-sensitive immunodetection of 5′methyl cytosine for DNA methylation analysis on oligonucleotide microarrays

DNA Research

Volumn 13, Issue 1, 2006, Pages 37-42

  Pröll, Johannes ^a,b   Födermayr, Mathilde ^a,b   Wechselberger, Christian ^b   Pammer, Patrick ^b   Sonnleitner, Max ^b   Zach, Otto ^a   Lutz, Dieter ^a  

^a ELISABETHINEN HOSPITAL   (Austria)

^b Upper Austrian Research GmbH   (Austria)

Author keywords

DNA methylation; Epigenetics; Immunofluorescence; Oligonucleotide microarray

Indexed keywords

5' METHYLCYTOSINE; CYTOSINE DERIVATIVE; GENOMIC DNA; PROTEIN P15; PROTEIN P16; UNCLASSIFIED DRUG; UVOMORULIN;

ARTICLE; AUTOFLUORESCENCE; DNA HYBRIDIZATION; DNA METHYLATION; HUMAN; HUMAN CELL; IMMUNODETECTION; IMMUNOFLUORESCENCE; LEUKEMIA CELL LINE; MICROARRAY ANALYSIS; MYELOID LEUKEMIA; REGULATORY DNA SEQUENCE; SENSITIVITY AND SPECIFICITY;

5-METHYLCYTOSINE; ANTIBODY SPECIFICITY; DNA METHYLATION; FEASIBILITY STUDIES; HL-60 CELLS; HUMANS; MODELS, BIOLOGICAL; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 33744938664     PISSN: 13402838     EISSN: 13402838     Source Type: Journal    
DOI: 10.1093/dnares/dsi024     Document Type: Article

References (22)

1. Ferguson-Smith, A. C. and Surani, M. A. 2001, Imprinting and the epigenetic asymmetry between parental genomes, Science, 293, 1086-1089.
2. Lee, J. T. 2003, Molecular links between X-inactivation and autosomal imprinting: X-inactivation as a driving force for the evolution of imprinting? Curr. Biol., 13, R242-254.
3. Issa, J. P. 2003, Age-related epigenetic changes and the immune system, Clin. Immunol., 109, 103-108.
4. Robertson, K. D. 2005, DNA methylation and human disease, Nat. Rev. Genet., 6, 597-610.
5. Ushijinia, T. 2005, Detection and interpretation of altered methylation patterns in cancer cells, Nat. Rev. Cancer, 5, 223-231.
6. Shiraishi, M. and Hayatsu, H. 2004, High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation, DNA Res., 11, 409-415.
7. Derks, S., Lentjes, M. H., Hellebrekers, D. M., de Bruine, A. P., Herman, J. G., and van Engeland, M. 2004, Methylation-specific PCR unraveled, Cell Oncol., 26, 291-299.
8. Hinterdorfer, P., Schutz, G., Kienberger, F., and Schindler, H. 2001, Detection and characterization of single biomolecules at surfaces, J. Biotechnol., 82, 25-35.
9. Hesse, J., Sonnleitner, M., and Sonnleitner, A. 2004, Single-molecule reader for high-throughput bioanalysis, Anal. Chem., 76, 5960-5964.
10. Cottier, M., Tchirkov, A., Perissel, B., Giollant, M., Campos, L., and Vago, P. 2004, Cytogenetic characterization of seven human cancer cell lines by combining G- and R-banding, M-FISH, CGH and chromosome- and locus-specific FISH, Int. J. Mol. Med., 14, 483-495.
11. Mrozek, K., Tanner, S. M., Heinonen, K., and Bloomfield, C. D. 2003, Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization, Genes Chromosomes Cancer, 38, 249-252.
12. Farinha, N. J., Shaker, S., Lemaire, M., Momparler, L., Bernstein, M., and Momparler, R. L. 2004, Activation of expression of p15, p73 and E-cadherin in leukemic cells by different concentrations of 5-aza-2(-deoxycytidine (Decitabine), Anticancer Res., 24, 75-78.
13. Dodge, J. E., Munson, C., and List, A. F. 2001, KG-1 and KG-1a model the p15 CpG island methylation observed in acute myeloid leukemia patients, Leuk. Res., 25, 917-925.
14. Herman, J. G., Graff, J. R., Myohanen, S., Nelkin, B. D., and Baylin, S. B. 1996, Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands, Proc. Natl Acad. Sci. USA, 93, 9821-9826.
15. Schlapak, R., Pammer, P., and Armitage, D. 2006, Glass surface grafted with high-density poly(ethylene glycol) as substrates for DNA oligonucleotide microarrays, Langmuir., 22, 277-285.
16. Takai, D. and Jones, P. A. 2003, The CpG island searcher: a new WWW resource, In Silico Biol., 3, 235-240.
17. Pieler, R., Sanchez-Cabo, F., Hackl, H., Thallinger, G. G., and Trajanoski, Z. 2004, ArrayNorm: comprehensive normalization and analysis of microarray data, Bioinformatics, 20, 1971-1973.
18. Reynaud, C., Bruno, C., Boullanger, P., Grange, J., Barbesti, S., and Niveleau, A. 1992, Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies, Cancer Lett., 61, 255-262.
19. Hardiman, G. 2003, Microarray methods and applications, Ist ed. DNA-press, Eagleville, Pennsylvania. 99-114.
20. Olek, A., Oswald, J., and Walter, J. 1996, A modified and improved method for bisulphite based cytosine methylation analysis, Nucleic Acids Res., 24, 5064-5066.
21. Graziano, F., Arduini, F., Ruzzo, A., et al. 2004, Prognostic analysis of E-cadherin gene promoter hypermethylation in patients with surgically resected, node-positive, diffuse gastric cancer, Clin. Cancer Res., 10, 2784-2789.
22. Brazma, A., Hingamp, P., Quackenbush, J., et al. 2001, Minimum information about a microarray experiment (MIAME)-toward standards for microarray data, Nat. Genet., 29, 365-371.