A simple non-destructive method for the fixation and immunostaining of cultured cells encapsulated in alginate

Biotechnology and Applied Biochemistry

Volumn 44, Issue 3, 2006, Pages 143-150

  Leal Egana, Aldo ^a   Heinrich, J Michael ^a   Smith, Mark D ^b   Nowicki, Marcin ^a   Bader, Augustinus ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b Haus 42   (Germany)

Author keywords

Biological markers; Cells encapsulated in alginate; Fluorescent antibody technique; Immobilized co culture; Immunohistochemistry

Indexed keywords

BIOMARKERS; CELL CULTURE; CELL MEMBRANES; CELLS; OPTIMIZATION; PROTEINS;

BIOLOGICAL MARKERS; CELLS ENCAPSULATED IN ALGINATE; FLUORESCENT-ANTIBODY TECHNIQUES; IMMOBILIZED CO-CULTURE; IMMUNOHISTOCHEMISTRY;

CYTOLOGY;

ALBUMIN; ALGINIC ACID; ALPHA FETOPROTEIN; BUFFER; CELL PROTEIN; THY 1 ANTIGEN;

ARTICLE; CANCER CELL CULTURE; CELL CULTURE; CELL ENCAPSULATION; CELL MEMBRANE; CELL STRUCTURE; COCULTURE; COLORIMETRY; CONTROLLED STUDY; EXTRACELLULAR SPACE; FIBROBLAST; FLUOROMETRY; HEPATOMA CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; INTRACELLULAR SPACE; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

ALBUMINS; ALGINATES; ALPHA-FETOPROTEINS; ANTIGENS, THY-1; CELL LINE, TUMOR; COCULTURE TECHNIQUES; FIBROBLASTS; GLUCURONIC ACID; HEXURONIC ACIDS; HUMANS; IMMUNOHISTOCHEMISTRY; STAINING AND LABELING; TISSUE FIXATION;

EID: 33744901478     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20050175     Document Type: Article

References (26)

1. Kamelger, F., Marksteiner R., Marksteiner, E., Klima, G., Wechselberger, G., Hering, S. and Piza, H. (2004) Biomaterials 25, 1649-1655
2. Stocum, D. (1998) Wound Repair Regen. 6, 276-290
3. De Bartolo, L., Morelli, S., Lopez, L. C., Giorno, L., Campana, C., Salerno, S., Rende, M., Favia, P., Detomaso, L., Gristina, R. et al. (2005) Biomaterials 26, 4432-4441
4. De Bartolo, L., Morelli, S., Gallo, M. C., Campana, C., Statti, G., Rende, M., Salerno, S. and Drioli, E. (2005) Biomaterials 26, 6625-6634
5. Semino, C., Merok, J., Crane, G., Panagiotakos, G. and Zhang, S. (2003) Differentiation 71, 262-270
6. Park, K. H., Na, K., Kim, S., Jung, S., Park, K. H. and Chung, H. M. (2005) Biotechnol. Lett. 27, 1081-1086
7. Itle, L., Koh, W. and Pishko, M. (2005) Biotechnol. Prog. 21, 926-932
8. Uchida, T., Serizawa, T., Ise, H., Akaike, T. and Akashi, M. (2001) Biomacromolecules 2, 1343-1346
9. Kim, T., Park, I., Nah, J., Choi, Y. and Cho, C. (2004) Biomaterials 25, 3783-3792
10. Gu, H., Chen, Z., Sa, R., Yuan, S., Chen, H., Ding, Y. and Yu, A. (2004) Biomaterials 25, 3445-3451
11. Murtas, S., Capuani, G., Dentini, M., Manetti, C., Masci, G., Massimi, M., Miccheli, A. and Crescenzi, V. (2005) J. Biomater. Sci. Polym. Ed. 16, 829-846
12. Lee, C., Wang, Y., Kuo, S. and Chang, S. (2004) Artif. Organs 28, 537-542
13. Hsu, S., Whu, S., Hsieh, S., Lin-Tsai, C., Chen, D. and Tan, T. (2004) Artif. Organs 28, 693-703
14. Rahman, T., Selden, C., Khalil, M., Diakanov, I. and Hodgson, H. (2004) Artif. Organs 28, 476-482
15. Falasca, L., Miccheli, A., Sartori, E., Tomassini, A. and Conti-Devirgiliis, L. (2001) Cell Tissue Org. 168, 126-136
16. Stensvaag, V., Furmanek, T., Lonning, K., Terzis, A. J., Bjerkvig, R. and Visted, T. (2004) Cell Transplant. 13, 35-44
17. Guyomard, C., Rialland, L., Fremond, B., Chesne, C. and Guillouzo, A. (1996) Toxicol. Appl. Pharmacol. 141, 349-356
18. Tu, R., Lu, C. L., Thyagarajan, K., Wang, E., Nguyen, H., Shen, S., Hata, C. and Quijano, R. C. (1993) Biomed. Mater. Res. 27, 3-9
19. Betigeri, S. and Neau, S. (2002) Biomaterials. 23, 3627-3636
20. Hand, A. R. and Hassell, J. R. (1976) J. Histochem. Cytochem. 24, 1000-1011
21. Normand, J. and Karasek, M. (1995) In Vitro Cell Dev. Biol. Anim. 31, 447-455
22. Chang, T. (1964) Science 164, 524-525
23. Chang, T. (1999) Ann. N.Y. Acad. Sci. 18, 875-883
24. Grossgebauer, K. (1979) Microsc. Acta 82, 291-293
25. Rajaonarivony, M., Vauthier, C., Couarraze, G., Puisieux, F. and Couvreur, P. (1993) J. Pharm. Sci. 82, 912-917
26. González-Ferreiro, M., Tillman, L., Hardee, G. and Bodmeier, R. (2002) Int. J. Pharm. 239, 47-59