The establishment and identification of the stable transfectant of HeLa cell expressing human new gene (PCIA1)

Journal of Sichuan University (Medical Science)

Volumn 37, Issue 3, 2006, Pages 335-338

  Wang, Rui ^a   Deng, Hong Xin ^a   Wang, Guo Qing ^a   Yan, Fei ^a   Peng, Feng ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Plasmid vector construction; Transfection

Indexed keywords

ANTIBIOTIC G 418; COMPLEMENTARY DNA; LIPOFECTAMINE; RNA;

ARTICLE; CANCER CELL CULTURE; GENE; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; OPEN READING FRAME; PCIA1 GENE; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

EID: 33744815648     PISSN: 1672173X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Wolfsberg TG, McEntyre J, Schuler GD. Guide to the draft human genome. Nature, 2001;409(15):824-826.
2. Zahra A, Alireza A, Shahana A, et al. Finishing the euchromatic sequence of the humangenome. Nature, 2004;431 (21):931-945.
3. Sahin U, Tureci O, Schmitt H, et al. Human neoplasms elicit multiple specific immune responses in the autologous host. Proc Natl Acad Sci U S A, 1995;92(25):11810-11813.
4. Naora H, Yang YQ, Montz FJ, et al. A serologically identified tumor antigen encoded by a homeobox gene promotes growth of ovarian epithelial cells. Proc Natl Acad Sci USA, 2001;98(7):4060-4065.
5. Chen JL, Dunbar PR, Gileadi U, et al. Identification of NY-ESO-1 peptide analogues capable of improved stimulation of tumor-reactive CTL. J Immunol, 2000;165(2):948-955.
6. Huang JJ, Lin MC, Bai YX, et al. Ectopic expression of a COOH-terminal fragment of the human telomerase reverse transcriptase leads to telomere dysfunction and reduction of growth and tumorigenicity in HeLa cells. Cancer Res,2002;62 (11):3226-3232.
7. Reading NS, Jenson SD, Smith JK, et al. 5′-(RACE) identification of rare ALK fusion partner in anaplastic large cell lymphoma. J Mol Diagn, 2003;5(2):136-140.
8. Skinner TL, Kerns RT, Bender PK. Three different calmodulin-encoding cDNAs isolated by a modified 5′-RACE using degenerate oligodeoxyribonucleotides. Gene, 1994;151 (1-2):247-251.
9. Park DJ. A new 5′ terminal murine GAPDH exon identified using 5′ RACE LaNe. Mol Biotechnol,2005;29(1):39-46.