Morphological evaluation of the radioprotective effects of melatonin against X-ray-induced early and acute testis damage in Albino rats: An animal model

International Journal of Experimental Pathology

Volumn 87, Issue 3, 2006, Pages 237-250

  Hussein, Mahmoud R ^a,d   Abu Dief, Eman E ^b   Abou El Ghait, Amal T ^a   Adly, Mohamed A ^b   Abdelraheem, Mohamad H ^c  

^a Assiut University Faculty of Medicine   (Egypt)

^b SOHAG UNIVERSITY   (Egypt)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^d KING KHALID UNIVERSITY   (Saudi Arabia)

Author keywords

Melatonin; Testis; X ray irradiation

Indexed keywords

ANIMALS; APOPTOSIS; CYTOPLASM; MALE; MELATONIN; MICROSCOPY, ELECTRON, TRANSMISSION; MITOCHONDRIA; MODELS, ANIMAL; RADIATION-PROTECTIVE AGENTS; RATS; RATS, MUTANT STRAINS; TESTIS; TIME FACTORS; X-RAYS;

EID: 33646863890     PISSN: 09599673     EISSN: 13652613     Source Type: Journal    
DOI: 10.1111/j.1365-2613.2006.00480.x     Document Type: Article

References (54)

1. Allan D.J. Gobe G.C. Harmon B.V. 1988 Sertoli cell death by apoptosis in the immature rat testis following x-irradiation. Scanning Microsc 2, 503 512.
2. van Alphen M.M. van de Kant H.J. de Rooij D.G. 1988 Depletion of the spermatogonia from the seminiferous epithelium of the rhesus monkey after X irradiation. Radiat Res. 113, 473 486.
3. Bain J. Keene J. 1975 Further evidence for inhibin: change in serum luteinizing hormone and follicle-stimulating hormone levels after x-irradiation of rat testis. J. Endocrinol. 66, 279 280.
4. Bansal M.R. Kaul A. Tewari M. Nehru B. 1990 Spermatogenesis and epididymal sperm after scrotal gamma irradiation in adult rats. Reprod Toxicol. 4, 321 324.
5. Beltran G. Stuckey W.J. 1972 Nuclear lobulation and cytoplasmic fibrils in leukemic plasma cells. Am. J. Clin. Pathol 58, 159 164.
6. Bessis M. 1985 Role of the cytoplasm fibrils in lobulation of the cell nucleus (formation of Rieder's cells). C R. Acad. Sci. Hebd Seances Acad. Sci. D 261, 1392 1393.
7. Beumer T.L. Roepers-Gajadien H.L. Gademan L.S. Rutgers D.H. de Rooij D.G. 1997 P21 (Cip1/WAF1) expression in the mouse testis before and after X irradiation. Mol Reprod Dev 47, 240 247.
8. Clifton D.K. Bremner W.J. 1983 The effect of testicular x-irradiation on spermatogenesis in man. A comparison with the mouse. J. Androl. 4, 387 392.
9. Cordelli E. Fresegna A.M. Leter G. Eleuteri P. Spano M. Villani P. 2003 Evaluation of DNA damage in different stages of mouse spermatogenesis after testicular X irradiation. Radiat Res. 160, 443 451.
10. Creasy D.M. 1997 Evaluation of testicular toxicity in safety evaluation studies: the appropriate use of spermatogenic staging. Toxicol. Pathol 25, 119 131.
11. Evdokimov V.V. Kodentsova V.M. Vrzhezinskaia O.A. Iakushina L.M. Erasova V.I. Kirpatovskii V.I. Sakharov I. 1997 [Effect of irradiation on the vitamin status and spermatogenesis in rats]. Biull Eksp Biol. Med. 123, 524 527.
12. Ghadially F.N. Senoo A. Fuse Y. 1985 A serial section study of nuclear pockets containing nuclear material. J. Submicrosc Cytol. 17, 687 694.
13. Giwercman A. von der Maase H. Berthelsen J.G. Rorth M. Bertelsen A. Skakkebaek N.E. 1991 Localized irradiation of testes with carcinoma in situ: effects on Leydig cell function and eradication of malignant germ cells in 20 patients. J. Clin. Endocrinol. Metab 73, 596 603.
14. Guitton N. Brouazin-Jousseaume V. Dupaix A. Jegou B. Chenal C. 1999 Radiation effect on rat Sertoli cell function in vitro and in vivo. Int. J. Radiat Biol. 75, 327 333.
15. Guitton N. Touzalin A.M. Sharpe R.M. Cheng C.Y. Pinon-Lataillade G. Meritte H. Chenal C. Jegou B. 2000 Regulatory influence of germ cells on sertoli cell function in the pre-pubertal rat after acute irradiation of the testis. Int. J. Androl. 23, 332 339.
16. Hatier R. Grignon G. Touati F. 1982 Ultrastructural study of seminiferous tubules in the rat after prenatal irradiation. Anat Embryol (Berl) 165, 425 435.
17. Hickman A.B. Klein D.C. Dyda F. 1999 Melatonin biosynthesis: the structure of serotonin N-acetyltransferase at 2.5 A resolution suggests a catalytic mechanism. Mol Cell 3, 23 32.
18. Hussein M.R. 2005 Apoptosis in the ovary: molecular mechanisms. Hum Reprod Update 11, 162 177.
19. Hussein M.R. Abu-Dief E.E. Abd El-Reheem M.H. Abd-Elrahman A. 2005 Ultrastructural evaluation of the radioprotective effects of melatonin against X-ray-induced skin damage in Albino rats. Int. J. Exp Pathol 86, 45 55.
20. Hussein M.R. Hassan M. Wood G.S. 2003 Morphological changes and apoptosis in radial growth phase melanoma cell lines following ultraviolet-B irradiation. Am. J. Dermatopathol 25, 466 472.
21. Karbownik M. Reiter R.J. Qi W. Garcia J.J. Tan D.X. Manchester L.C., Vijayalaxmi 2000 Protective effects of melatonin against oxidation of guanine bases in DNA and decreased microsomal membrane fluidity in rat liver induced by whole body ionizing radiation. Mol Cell Biochem. 211, 137 144.
22. Kerr J.F. Wyllie A.H. Currie A.R. 1972 Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26, 239 257.
23. Kim B.C. Shon B.S. Ryoo Y.W. Kim S.P. Lee K.S. 2001 Melatonin reduces X-ray irradiation-induced oxidative damages in cultured human skin fibroblasts. J. Dermatol. Sci. 26, 194 200.
24. Koc M. Buyukokuroglu M.E. Taysi S. 2002 The effect of melatonin on peripheral blood cells during total body irradiation in rats. Biol. Pharm. Bull. 25, 656 657.
25. Love R. Soriano R.Z. 1971 Correlation of nucleolini with fine structural nucleolar constituents of cultured normal and neoplastic cells. Cancer Res. 31, 1030 1037.
26. Montironi R. Braccischi A. Matera G. Scarpelli M. Pisani E. 1991 Quantitation of the prostatic intra-epithelial neoplasia. Analysis of the nucleolar size, number and location. Pathol Res. Pract 187, 307 314.
27. Mornjakovic Z. Alicelebic S. Bilalovic N. Susko I. 1998 [Morphometric characteristics of Leydig cells after total irradiation of rats treated with melatonin]. Med. Arh 52, 183 184.
28. Mornjakovic Z. Scepovic M. Kundurovic Z. 1991 [Morphometric aspects of seminiferous tubules in rats treated with melatonin and whole body irradiation (stereologic analysis) ]. Med. Arh 45, 9 10.
29. Omura M. Romero Y. Zhao M. Inoue N. 1999 Histopathological evidence that spermatogonia are the target cells of 2-bromopropane. Toxicol. Lett. 104, 19 26.
30. Petersen R.G. 1985 Design and Analysis of Experiments. Marcil Dekker,Inc, New york.
31. Pineau C. VeleZ. de la Calle J.F. Pinon-Lataillade G. Jegou B. 1989 Assessment of testicular function after acute and chronic irradiation: further evidence for an influence of late spermatids on Sertoli cell function in the adult rat. Endocrinology 124, 2720 2728.
32. Pinon-Lataillade G. Maas J. 1985 Continuous gamma-irradiation of rats: dose-rate effect on loss and recovery of spermatogenesis. Strahlentherapie 161, 421 426.
33. Pinon-Lataillade G. Viguier-MartineZ. M.C. Maas J. 1985 Endocrinological and histological changes induced by continuous low dose gamma-irradiation of the rat testis. Acta Endocrinol. (Copenh) 109, 558 562.
34. Ponnapakkam T.P. Sam G.H. Iszard M.B. 2003 Histopathological changes in the testis of the Sprague Dawley rat following orally administered manganese. Bull. Environ. Contam Toxicol. 71, 1151 1157.
35. Reddy J. Svoboda D. 1968 The relationship of nucleolar segregation to ribonucleic acid synthesis following the administration of selected hepatocarcinogens. Laboratory Invest 19, 32 45.
36. Reiter R.J. Tan D.X. Mayo J.C. SainZ. R.M. Leon J. Czarnocki Z. 2003 Melatonin as an antioxidant: biochemical mechanisms and pathophysiological implications in humans. Acta Biochim. Pol 50, 1129 1146.
37. Ressmeyer A.R. Mayo J.C. Zelosko V. SainZ. R.M. Tan D.X. Poeggeler B. Antolin I. Zsizsik B.K. Reiter R.J. Hardeland R. 2003 Antioxidant properties of the melatonin metabolite N1-acetyl-5-methoxykynuramine (AMK): scavenging of free radicals and prevention of protein destruction. Redox Rep 8, 205 213.
38. Sawada H. Esaki M. 2003 Electron microscopic observation of 137Cs-irradiated rat testis: production of basal laminae for germ cells, despite their absence. J. Electron Microsc (Tokyo) 52, 391 397.
39. Sener G. Jahovic N. Tosun O. Atasoy B.M. Yegen B.C. 2003 Melatonin ameliorates ionizing radiation-induced oxidative organ damage in rats. Life Sci. 74, 563 572.
40. Shalet S.M. 1993 Effect of irradiation treatment on gonadal function in men treated for germ cell cancer. Eur Urol. 23, 148 151 ; discussion 152.
41. Simpson G.G. Roe A. Lewontin R.C. 1960) Quantitative Zoology. New York,Harcourt,Brace & World. Inc.
42. Songthaveesin C. Saikhun J. Kitiyanant Y. Pavasuthipaisit K. 2004 Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study. Asian J. Androl. 6, 331 336.
43. Taysi S. Koc M. Buyukokuroglu M.E. Altinkaynak K. Sahin Y.N. 2003 Melatonin reduces lipid peroxidation and nitric oxide during irradiation-induced oxidative injury in the rat liver. J. Pineal Res. 34, 173 177.
44. Teodori L. Tagliaferri F. Stipa F. Valente M.G. Coletti D. Manganelli A. Guglielmi M. D'Angelo L.S. Schafer H. Gohde W. 2000 Selection, establishment and characterization of cell lines derived from a chemically-induced rat mammary heterogeneous tumor, by flow cytometry, transmission electron microscopy, and immunohistochemistry. In Vitro Cell Dev Biol. Anim 36, 153 162.
45. Undeger U. Giray B. Zorlu A.F. Oge K. Bacaran N. 2004 Protective effects of melatonin on the ionizing radiation induced DNA damage in the rat brain. Exp Toxicol. Pathol 55, 379 384.
46. Vachhrajani K.D. Dutta K.K. 1992 Stage specific effect during one seminiferous epithelial cycle following ethylene glycol monomethyl ether exposure in rats. Indian J. Exp Biol. 30, 892 896.
47. Vijayalaxmi, Meltz M.L. Reiter R.J. Herman T.S. 1999a Melatonin and protection from genetic damage in blood and bone marrow: whole-body irradiation studies in mice. J. Pineal Res. 27, 221 225.
48. Vijayalaxmi, Seaman R.L. Belt M.L. Doyle J.M. Mathur S.P. Prihoda T.J. 1999b Frequency of micronuclei in the blood and bone marrow cells of mice exposed to ultra-wideband electromagnetic radiation. Int. J. Radiat Biol. 75, 115 120.
49. West A. Lahdetie J. 2001 X-irradiation - induced changes in the progression of type B spermatogonia and preleptotene spermatocytes. Mol Reprod Dev 58, 78 87.
50. West A. Priante G. Lahdetie J. 2002 Stage-specific expression of Gadd45 induced by X-irradiation in rat spermatogenesis. Int. J. Radiat Biol. 78, 29 39.
51. Zatsepina O.V. Voronkova L.N. Sakharov V.N. Chentsov Y.S. 1989 Ultrastructural changes in nucleoli and fibrillar centers under the effect of local ultraviolet microbeam irradiation of interphase culture cells. Exp Cell Res. 181, 94 104.
52. Zhao M. Omura M. Tokunaga S. Romero Y. Inoue N. 1998 Histopathological changes within the testis caused by allyl chloride exposure in mice. Bull. Environ. Contam Toxicol. 60, 494 501.
53. Zichner L. Engel D. 1971 Electron microscopial examination of the ultra-sonic effect on the rabbit's synovial membrane. Z. Gesamte Exp Med. 154, 1 13.
54. Zucker-Franklin D. Melton J.W. 3rd Quagliata F. 1974 Ultrastructural, immunologic, and functional studies on Sezary cells: a neoplastic variant of thymus-derived (T) lymphocytes. Proc. Natl Acad. Sci. U S A 71, 1877 1881.