Letter to the glyco-forum: Preparation of CD25+ and CD25- CD4+ T cells for glycomic analysis - A cautionary tale of serum glycoprotein sequestration

Glycobiology

Volumn 16, Issue 6, 2006, Pages

  Monk, C R ^a   Sutton Smith, M ^a   Dell, A ^a   Garden, O A ^b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b ROYAL VETERINARY COLLEGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; GLYCAN; GLYCOPROTEIN; INTERLEUKIN 2 RECEPTOR ALPHA; BUFFER; INTERLEUKIN 2 RECEPTOR; POLYSACCHARIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CD25+ T LYMPHOCYTE; CD4+ CD25+ T LYMPHOCYTE; CELL CULTURE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CULTURE MEDIUM; FETAL CALF SERUM; GLYCOBIOLOGY; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; ANIMAL; BLOOD; C57BL MOUSE; FEMALE; IMMUNOLOGY; IN VITRO STUDY; LETTER; METABOLISM; T LYMPHOCYTE;

ANIMALIA;

ANIMALS; ANTIGENS, CD4; BUFFERS; FEMALE; GLYCOPROTEINS; MICE; MICE, INBRED C57BL; POLYSACCHARIDES; RECEPTORS, INTERLEUKIN-2; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; T-LYMPHOCYTES;

EID: 33646862409     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwj101     Document Type: Article

References (5)

1. Comelli, E.M., Head, S.R., Gilmartin, T., Whisenant, T., Haslam, S.M., North, S.J., Wong, N.K., Kudo, T., Narimatsu, H., Esko, J.D., and others (2005) A focused microarray approach to functional glycomics: transcriptional regulation of the glycome. Glycobiology, 24, 24.
2. Kawano, T., Koyama, S., Takematsu, H., Kozutsumi, Y., Kawasaki, H., Kawashima, S., Kawasaki, T., and Suzuki, A. (1995) Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolyl-neuraminic acid. J. Biol. Chem., 270, 16458-16463.
3. Robbins, P.W. and Samuelson, J. (2005) Asparagine linked glycosylation in Giardia. Glycobiology, 15, 15G-16G.
4. Rudd, P.M., Elliott, T., Cresswell, P., Wilson, I.A., and Dwek, R.A. (2001) Glycosylation and the immune system. Science, 291, 2370-2376.
5. Rudd, P.M., Wormald, M.R., and Dwek, R.A. (2004) Sugar-mediated ligand-receptor interactions in the immune system. Trends Biotechnol., 22, 524-530.