Rapid discrimination of cytK-1 and cytK-2 genes in Bacillus cereus strains bya novel duplex PCR system

FEMS Microbiology Letters

Volumn 259, Issue 1, 2006, Pages 74-80

  Guinebretiere, Marie Hélène ^a   Fagerlund, Annette ^b   Granum, Per E ^b   Nguyen The, Christophe ^a  

^a UMR A 408 INRA University of Avignon   (France)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

Author keywords

Bacillus cereus; cytK 1 gene; cytK 2 gene; Cytotoxin K; Detection; Diarrhoeal food poisoning; PCR

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACILLUS CEREUS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; CYTK 1 GENE; CYTK 2 GENE; DNA ISOLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOUTHERN BLOTTING; STRAIN IDENTIFICATION; VALIDATION PROCESS;

BACILLUS CEREUS; BACTERIAL TOXINS; BASE SEQUENCE; DNA PRIMERS; GENES, BACTERIAL; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; TIME FACTORS;

BACILLUS CEREUS;

EID: 33646716664     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00247.x     Document Type: Article

References (18)

1. Altschul SF Madden TL Schäffer AA Zhang J Zhang Z Miller W Lipman DJ 1997 Gapped BLAST and PSI-BLAST a new generation of protein database search programs Nucleic Acids Res 25 3389 3402
2. Buchanan RL Schultz FJ 1992 Evaluation of the oxoid Bcet-Rpla Kit for the detection of Bacillus cereus diarrheal enterotoxin as compared to cell-culture cytotonicity J Food Protect 55 440 443
3. Buchanan RL Schultz FJ 1994 Comparison of the Tecra-Via Kit, Oxoid Bcet-Rpla Kit and Cho cell-culture assay for the detection of Bacillus cereus diarrheal enterotoxin Lett Appl Microbiol 19 353 356
4. Corona A Fois MP Mazzette R De Santis EPL 2003 A new multiplex PCR for the detection of hbl genes in strains of the 'Bacillus cereus group' Vet Res Commun 27 679 682
5. Corpet F 1988 Multiple sequence alignment with hierarchical clustering Nucleic Acids Res 16 10881 10890
6. Dietrich R Fella C Strich S Martlbauer E 1999 Production and characterization of monoclonal antibodies against the hemolysin BL enterotoxin complex produced by Bacillus cereus Appl Environ Microbiol 65 4470 4474
7. Dietrich R Moravek M Bürk C Granum PE Märtlbauer E 2005 Production and characterization of antibodies against each of the three subunits of the Bacillus cereus nonhemolytic enterotoxin complex Appl Environ Microbiol 71 8214 8220
8. Ehling-Schulz M Fricker M Scherer S 2004 Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay FEMS Microbiol Lett 232 189 195
9. Ehling-Schulz M Vukov N Schulz A Shaheen R Andersson M Martlbauer E Scherer S 2005 Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus Appl Environ Microbiol 71 105 113
10. Fagerlund A Ween A Lund T Hardy SP Granum PE 2004 Genetic and functional analysis of the cytK family of genes in Bacillus cereus Microbiology 150 2689 2697
11. Guinebretiere MH Nguyen-The C 2003 Sources of Bacillus cereus contamination in a pasteurized zucchini puree processing line, differentiated by two PCR-based methods FEMS Microbiol Ecol 43 207 215
12. Guinebretiere MH Broussolle V Nguyen-The C 2002 Enterotoxigenic profiles of food-poisoning and food-borne Bacillus cereus strains J Clin Microbiol 40 3053 3056
13. Hansen BM Hendriksen NB 2001 Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis Appl Environ Microbiol 67 185 189
14. Henegariu O Heerema NA Dlouhy SR Vance GH Vogt PH 1997 Multiplex PCR critical parameters and step-by-step protocol BioTechniques 23 504 511
15. Lund T De Buyser ML Granum PE 2000 A new cytotoxin from Bacillus cereus that may cause necrotic enteritis Mol Microbiol 38 254 261
16. Moravek M Wegscheider M Schulz A Dietrich R Burk C Martlbauer E 2004 Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus FEMS Microbiol Lett 238 107 113
17. Sambrook J Fritsch EF Maniatis T 1989 Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory New York
18. Thompson JD Gibson TJ Plewniak F Jeanmougin F Higgins DG 1997 The CLUSTAL X windows interface flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 25 4876 4882