Visual and PCR assessment of light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape (Brassica napus) cultivars

Plant Pathology

Volumn 55, Issue 3, 2006, Pages 387-400

  Karolewski, Z ^a,b   Fitt, B D L ^a   Latunde Dada, A O ^a   Foster, S J ^a,c   Todd, A D ^a   Downes, K ^a   Evans, N ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b POZNA UNIVERSITY OF LIFE SCIENCES   (Poland)

^c SAINSBURY LABORATORY   (United Kingdom)

Author keywords

Cultivar resistance; Disease assessment; PCR diagnostics; Plant disease symptomatology; Rapeseed; Sporulation

Indexed keywords

CULTIVAR; DISEASE RESISTANCE; LEAF;

BACTERIA (MICROORGANISMS); BRASSICA NAPUS; PYRENOPEZIZA BRASSICAE; SINAPIS ARVENSIS;

EID: 33646706066     PISSN: 00320862     EISSN: 13653059     Source Type: Journal    
DOI: 10.1111/j.1365-3059.2006.01383.x     Document Type: Article

References (32)

1. Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389 402.
2. Amelung D, Daebeler F, 1991. Occurrence of fertile apothecia and the epidemiology of Pyrenopeziza brassicae Sutton and Rawlinson (anamorph: Cylindrosporium concentricum Grev.) in the German Democratic Republic. International Organisation for Biological Control Bulletin 14, 147 50.
3. Anonymous, 1998. Oilseed Rape Variety Handbook. Cambridge, UK:National Institute of Agricultural Botany.
4. Bradburne R, Majer D, Magrath R, Werner CP, Lewis B, Mithen R, 1999. Winter oilseed rape with high levels of resistance to Pyrenopeziza brassicae derived from wild Brassica species. Plant Pathology 48, 550 5.
5. Brun H, Renard M, Jouan B, Tanguy X, Lamarque C, 1979. Observations préliminaires sur quelque maladies du colza en France. Sclerotinia sclerotiorum, Cylindrosporium concentricum, Ramularia armoraciae. Sciences Agronomique de Rennes 1979, 7 17.
6. Dyer PS, Furneaux PA, Douhan G, Murray TD, 2001. A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis. Fungal Genetics and Biology 33, 173 80.
7. Evans N, Baeirl A, Brain P, Welham SJ, Fitt BDL, 2003. Spatial aspects of light leaf spot (Pyrenopeziza brassicae) epidemic development on winter oilseed rape (Brassica napus) in the UK. Phytopathology 93, 657 65.
8. Figueroa L, Fitt BDL, Shaw MW, McCartney HA, Welham SJ, 1995. Effects of temperature on the development of light leaf spot (Pyrenopeziza brassicae) on oilseed rape (Brassica napus). Plant Pathology 44, 51 62.
9. Fitt BDL, Doughty KJ, Gilles T, Gladders P, Steed JM, Su H, Sutherland KG, 1998b. Methods for assessment of light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape in the UK. Annals of Applied Biology 133, 329 42.
10. Fitt BDL, Doughty KJ, Gladders P, Steed JM, Sutherland KG, 1998a. Diagnosis of light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape (Brassica napus) in the UK. Annals of Applied Biology 133, 155 66.
11. Fitt BDL, Turner JA, Gladders P, Sutherland K, 1999. Relationships between regional weather and incidence of light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape in England and Wales. Aspects of Applied Biology 56, 102 6.
12. Foster SJ, Ashby AM, Fitt BDL, 2002. Improved PCR-based assays for pre-symptomatic diagnosis of light leaf spot and determination of mating type of Pyrenopeziza brassicae on winter oilseed rape. European Journal of Plant Pathology 108, 379 83.
13. Foster SJ, Singh G, Fitt BDL, Ashby AM, 1999. Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera). Physiological and Molecular Plant Pathology 55, 111 9.
14. Fraaije BA, Lovell DJ, Coelho JM, Baldwin S, Hollomon DW, 2001. PCR-based assays to assess wheat varietal resistance to blotch (Septoria tritici and Stagonospora nodorum) and rust (Puccinia striiformis and Puccinia recondita) diseases. European Journal of Plant Pathology 107, 905 17.
15. Gilles T, Evans N, Fitt BDL, Jeger MJ, 2000b. Epidemiology in relation to methods for forecasting light leaf spot (Pyrenopeziza brassicae) severity on winter oilseed rape (Brassica napus) in the UK. European Journal of Plant Pathology 106, 593 600.
16. Gilles T, Fitt BDL, Kennedy R, Welham SJ, Jeger MJ, 2000a. Effects of temperature and wetness duration on conidial infection, latent period and asexual sporulation of Pyrenopeziza brassicae on leaves of oilseed rape. Plant Pathology 49, 498 508.
17. Gilles T, Fitt BDL, McCartney HA, Papastamati K, Steed JM, 2001. The roles of ascospores and conidia of Pyrenopeziza brassicae in light leaf spot epidemics on winter oilseed rape (Brassica napus) in the UK. Annals of Applied Biology 138, 141 52.
18. Graham GC, Mayers P, Henry RJ, 1994. A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques 16, 48 50.
19. Karolewski Z, 1999. The occurrence of light leaf spot on winter oilseed rape in Western Poland in 1991-96 and the characteristics of Pyrenopeziza brassicae isolates. Phytopathologia Polonica 18, 113 21.
20. Karolewski Z, Evans N, Fitt BDL, Baierl A, Todd AD, Foster SJ, 2004. Comparative epidemiology of Pyrenopeziza brassicae (light leaf spot) ascospores and conidia from Polish and UK populations. Plant Pathology 53, 29 37.
21. Karolewski Z, Evans N, Fitt BDL, Todd AD, Baierl A, 2002. Sporulation of Pyrenopeziza brassicae (light leaf spot) on oilseed rape (Brassica napus) leaves inoculated with ascospores or conidia at different temperatures and wetness durations. Plant Pathology 51, 654 65.
22. Kenyon D, Thomas J, Handy C, 2004. Feasibility of using quantitative PCR for assessing resistance to stem canker in oilseed rape cultivars. International Organisation for Biological Control Bulletin 27, 109 17.
23. Lee SB, Taylor JW, 1990. Isolation of DNA from fungal mycelia and single spores. In:Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: a Guide to Methods and Applications. San Diego, CA, USA:Academic Press, 282 7.
24. Majer D, Lewis BG, Mithen R, 1998. Genetic variation among field isolates of Pyrenopeziza brassicae. Plant Pathology 47, 22 8.
25. Payne RW, Lane PW, Digby PGN, Harding SA, Leech PK, Morgan GW, Todd AD, Thompson R, Tunnicliffe Wilson G, Welham SJ, White RP, 1993 Genstat 5 Release 3 Reference Manual. Oxford, UK:Clarendon Press.
26. Pilet ML, Delourme R, Foisset N, Renard M, 1998. Identification of QTL involved in field resistance to light leaf spot (Pyrenopeziza brassicae) and blackleg resistance (Leptosphaeria maculans) in winter rapeseed (Brassica napus L.). Theoretical and Applied Genetics 97, 398 406.
27. Su H, Fitt BDL, Welham SJ, Sansford CE, Sutherland KG, 1998. Effects of light leaf spot (Pyrenopeziza brassicae) on yield of winter oilseed rape (Brassica napus). Annals of Applied Biology 132, 371 86.
28. Sutherland KG, Fitt BDL, Steed JM, Welham SJ, Gladders P, Turner JA, 1998. Development and control of light leaf spot (Pyrenopeziza brassicae) epidemics in winter oilseed rape in the UK. Brighton Crop Protection Conference - Pests and Diseases, 1998. Farnham, UK:BCPC, 1053 58.
29. Sylvester-Bradley R, Makepeace RJ, 1985. Revision of a code for stages of development in oilseed rape (Brassica napus). Aspects of Applied Biology 10, 395 400.
30. Thomas J, Kenyon D, Handy C, 2004. Preliminary results on the use of quantitative PCR for assessing resistance to light leaf spot (Pyrenopeziza brassicae) in oilseed rape cultivars. International Organisation for Biological Control Bulletin 27, 83 6.
31. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876 82.
32. White TJ, Bruns T, Lee S, Taylor J, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In:Innis MA, Gelfland DH, Sninsky JJ, White TJ, eds. PCR Protocols: a Guide to Methods and Applications. San Diego, CA, USA:Academic Press, 315 22.