Evidence denies the presence of O-GlcNAcylation on mouse glucocorticoid receptor and its potential involvement in receptor transcriptional activity

Journal of Receptors and Signal Transduction

Volumn 26, Issue 3, 2006, Pages 129-145

  Zhang, Shimin ^a   Danielsen, Mark ^b  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^b GEORGETOWN UNIVERSITY   (United States)

Author keywords

Chromatography and transcriptional activity; Glucocorticoid receptor; Immunoprecipitation; O GlcNAcylation; Wheat germ agglutinin

Indexed keywords

ANDROGEN RECEPTOR; CYTOPLASM PROTEIN; ESTROGEN RECEPTOR; GLUCOCORTICOID RECEPTOR; LECTIN; N ACETYLGLUCOSAMINE; NUCLEAR PROTEIN; STEROID RECEPTOR;

ACYLATION; AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; GENE EXPRESSION REGULATION; GENE MUTATION; GENE OVEREXPRESSION; HORMONE PROTEIN COMPLEX; MOUSE; NONHUMAN; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; RECEPTOR AFFINITY; SIGNAL TRANSDUCTION; TECHNIQUE; TRANSCRIPTION REGULATION; WESTERN BLOTTING;

ACETYLGLUCOSAMINE; ACYLATION; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; CELL LINE; CHO CELLS; CRICETINAE; MICE; MUTAGENESIS, SITE-DIRECTED; RECEPTORS, GLUCOCORTICOID; RECOMBINANT PROTEINS; TRANSCRIPTION, GENETIC;

ANIMALIA; TRITICUM AESTIVUM;

EID: 33646584830     PISSN: 10799893     EISSN: 15324281     Source Type: Journal    
DOI: 10.1080/10799890600623340     Document Type: Article

References (41)

1. Comer FI, Hart GW. O-glycosylation of nuclear and cytosolic proteins. Dynamic interplay between O-GlcNAc and O-phosphate. J Biol Chem 2000, 275, 29179-29182.
2. Iyer SP, Hart GW. Dynamic nuclear and cytoplasmic glycosylation: Enzymes of o-glcnac cycling. Biochemistry 2003, 42, 2493-2499.
3. Zachara NE, Cheung WD, Hart GW. Nucleocytoplasmic glycosylation, O-GlcNAc: Identification and site mapping. Methods Mol Biol 2004, 284, 175-194.
4. Kamemura K, Hart GW. Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: A new paradigm for metabolic control of signal transduction and transcription. Prog Nucleic Acid Res Mol Biol 2003, 73, 107-136.
5. Wells L, Vosseller K, Hart GW. Glycosylation of nucleocytoplasmic proteins: Signal transduction and O-GlcNAc. Science 2001, 291, 2376-2378.
6. Sohn KC, Do SI. Transcriptional regulation and o-glcnacylation activity of zebrafish ogt during embryogenesis. Biochem Biophys Res Commun 2005, 337, 256-263.
7. Shafi R, Iyer SP, Ellies LG, O'Donnell N, Marek KW, Chui D, Hart GW, Marth JD. The O-GlcNAc transferase gene resides on the x chromosome and is essential for embryonic stem cell viability and mouse ontogeny. Proc Natl Acad Sci U S A 2000, 97, 5735-5739.
8. Liu F, Iqbal K, Grundke-Iqbal I, Hart GW, Gong CX. O-glcnacylation regulates phosphorylation of tau: A mechanism involved in alzheimer's disease. Proc Natl Acad Sci U S A 2004, 101, 10804-10809.
9. Zhang SM, Li JX, Wei YM, Yang ZM. A simplified method for the preparation of wga-sepharose 4b and its use in the purification of mn blood group antigens. Biomed Chromatogr 1989, 3, 10-13.
10. Finlay DR, Newmeyer DD, Price TM, Forbes DJ. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores. J Cell Biol 1987, 104, 189-200.
11. Yang X, Su K, Roos MD, Chang Q, Paterson AJ, Kudlow JE. O-linkage of N-acetylglucosamine to sp1 activation domain inhibits its transcriptional capability. Proc Natl Acad Sci U S A 2001, 98, 6611-6616.
12. Jiang MS, Hart GW. A subpopulation of estrogen receptors are modified by o-linked n-acetylglucosamine. J Biol Chem 1997, 272, 2421-2428.
13. McCann JP, Mayes JS, Hendricks GR, Harjo JB, Watson GH. Subcellular distribution and glycosylation pattern of androgen receptor from sheep omental adipose tissue. J Endocrinol 2001, 169, 587-593.
14. Nealen ML, Vijayan KV, Bolton E, Bray PF. Human platelets contain a glycosylated estrogen receptor beta. Circ Res 2001, 88, 438-442.
15. Karthikeyan N, Thampan RV. The nuclear estrogen receptor R-II of the goat uterus: Distinct possibility that the R-II is the deglycosylated form of the nonactivated estrogen receptor (naER). Arch Biochem Biophys 1995, 321, 442-452.
16. Cheng X, Hart GW. Alternative O-glycosylation/O-phosphorylation of serine-16 in murine estrogen receptor beta: Post-translational regulation of turnover and transactivation activity. J Biol Chem 2001, 276, 10570-10575.
17. Danielsen M. Structure and Function of the Glucocorticoid Receptor. London, Academic Press, 1991.
18. Hirst MA, Northrop JP, Danielsen M, Ringold GM. High level expression of wild type and variant mouse glucocorticoid receptors in chinese hamster ovary cells. Mol Endocrinol 1990, 4, 162-170.
19. Westphal HM, Moldenhauer G, Beato M. Monoclonal antibodies to the rat liver glucocorticoid receptor. EMBO J 1982, 1, 1467-1471.
20. Harlow E, Lane D. Antibodies: A Laboratory Manual. Cold Spring Harbor New York, Cold Spring Harbor, Laboratory, 1988, 511-525.
21. Zhang S. Rapid purification of wheat germ agglutinin from wheat germ. J Med Colleges of PLA 1987, 2, 223-227.
22. Laemmli, UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
23. Blackshear PJ. Systems for polyacrylamide gel electrophoresis. Methods Enzymol 1984, 104, 237-255.
24. Zhang S, Liang X, Danielsen M. Role of the C terminus of the glucocorticoid receptor in hormone binding and agonist/antagonist discrimination. Mol Endocrinol 1996, 10, 24-34.
25. Whiteheart SW, Passaniti A, Reichner JS, Holt GD, Haltiwanger RS, Hart GW. Glycosyltransferase probes. Methods Enzymol 1989, 179, 82-95.
26. Roquemore EP, Chou TY, Hart GW. Detection of O-linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins. Methods Enzymol 1994, 230, 443-460.
27. Danielsen M, Northrop JP, Ringold GM. The mouse glucocorticoid receptor: Mapping of functional domains by cloning, sequencing and expression of wild-type and mutant receptor proteins. EMBO J 1986, 5, 2513-2522.
28. Mason SA, Housley PR. Site-directed mutagenesis of the phosphorylation sites in the mouse glucocorticoid receptor. J Biol Chem 1993, 268, 21501-21504.
29. Price J, Turner D, Cepko C. Lineage analysis in the vertebrate nervous system by retrovirus- mediated gene transfer. Proc Natl Acad Sci U S A 1987, 84, 156-160.
30. Zhang S, Lu J, Iyama K, Lo SC, Danielsen M. A simplified method for large scale quantification of transcriptional activity and its use in studies of steroids and steroid receptors. J Recept Signal Transduct Res 2001, 21, 71-84.
31. Jackson SP, Tjian R. Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography. Proc Natl Acad Sci U S A 1989, 86, 1781-1785.
32. Magee SC, Mawal R, Ebner KE. Multiple forms of galactosyltransferase from bovine milk. Biochemistry 1974, 13, 99-102.
33. Hart GW. Dynamic O-linked glycosylation of nuclear and cytoskeletal proteins. Annu Rev Biochem 1997, 66, 315-335.
34. Bodwell JE, Orti E, Coull JM, Pappin DJ, Smith LI, Swift F. Identification of phosphorylated sites in the mouse glucocorticoid receptor. J Biol Chem 1991, 266, 7549-7555.
35. Miesfeld R, Rusconi S, Godowski PJ, Maler BA, Okret S, Wikstrom AC, Gustafsson JA, Yamamoto KR. Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor CDNA. Cell 1986, 46, 389-399.
36. Yen PM, Simons SS Jr. Evidence against posttranslational glycosylation of rat glucocorticoid receptors. Receptor 1991, 1, 191-205.
37. Mendel DB, Bodwell JE, Gametchu B, Harrison RW, Munck A. Molybdate-stabilized nonactivated glucocorticoid-receptor complexes contain a 90-kda non-steroid-binding phosphoprotein that is lost on activation. J Biol Chem 1986, 261, 3758-3763.
38. Jackson SP, Tjian R. O-glycosylation of eukaryotic transcription factors: Implications for mechanisms of transcriptional regulation. Cell 1988, 55, 125-133.
39. Cheng X, Cole RN, Zaia J, Hart GW. Alternative O-glycosylation/O- phosphorylation of the murine estrogen receptor beta. Biochemistry 2000, 39, 11609-11620.
40. Bodwell JE, Webster JC, Jewell CM, Cidlowski JA, Hu JM, Munck A. Glucocorticoid receptor phosphorylation: Overview, function and cell. J Steroid Biochem Mol Biol 1998, 65, 91-99.
41. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72, 248-254.