Isolation and characterization of a new Clostridium sp. that performs effective cellulosic waste digestion in a thermophilic methanogenic bioreactor

Applied and Environmental Microbiology

Volumn 72, Issue 5, 2006, Pages 3702-3709

  Shiratori, Hatsumi ^a   Ikeno, Hironori ^a   Ayame, Shohei ^b   Kataoka, Naoaki ^b   Miya, Akiko ^b   Hosono, Kuniaki ^a   Beppu, Teruhiko ^a   Ueda, Kenji ^a  

^a NIHON UNIVERSITY   (Japan)

^b EBARA CORPORATION   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTORS; CARBON; FERMENTATION; GENES; MICROORGANISMS; RNA; WASTE DISPOSAL;

CLOSTRIDIUM STRAMINISOLVENS; METHANOGENIC BIOREACTORS; METHANOGENIC SLUDGES; WASTE DIGESTION;

BACTERIA;

CELLULOSE; RIBOSOME RNA;

BACTERIUM; CELLULOSE; FERMENTATION; MICROBIAL COMMUNITY; RNA; SLUDGE; SUBSTRATE;

ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATION; BIOREACTOR; CARBON SOURCE; CLOSTRIDIALES; CLOSTRIDIUM; CLOSTRIDIUM STRAMINISOLVENS; CLOSTRIDIUM THERMOCELLUM; FERMENTATION; GENE SEQUENCE; METHANOGENESIS; MICROFLORA; NONHUMAN; NUCLEOTIDE SEQUENCE; PAPER; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA GENE;

BIOREACTORS; CELLULOSE; CLOSTRIDIUM; DNA, BACTERIAL; HEAT; METHANE; MOLECULAR SEQUENCE DATA; PAPER; REFUSE DISPOSAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

CLOSTRIDIA; CLOSTRIDIALES; CLOSTRIDIUM SP.; CLOSTRIDIUM STRAMINISOLVENS; CLOSTRIDIUM THERMOCELLUM;

EID: 33646560608     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.72.5.3702-3709.2006     Document Type: Article

References (22)

1. Angelidaki, I., L. Ellegaard, and B. K. Ahring. 2003. Applications of the anaerobic digestion process, p. 1-33. In B. K. Ahring (ed.), Biomethanation II, vol. 82. Springer-Verlag, Berlin, Germany.
2. Boone, D., D. P. Chynoweth, R. A. Mah, P. H. Smith, and A. C. Wilkie. 1993. Ecology and microbiology of biogasification. Biomass Bioenerg. 5:191-202.
3. Burrell, P. C., C. O'Sullivan, H. Song, W. P. Clarke, and L. L. Blackall. 2004. Identification, detection, and spatial resolution of Clostridium populations responsible for cellulose degradation in a methanogenic landfill leachate bioreactor. Appl. Environ. Microbiol. 70:2414-2419.
4. Chin, K. J., D. Hahn, U. Hengstmann, W. Liesack, and P. H. Janssen. 1999. Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms. Appl. Environ. Microbiol. 65:5042-5049.
5. Cole, J. R., B. Chai, R. J. Farris, Q. Wang, S. A. Kulam, D. M. McGarrell, G. M. Garrity, and J. M. Tiedje. 2005. The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res. 33:D294-D296.
6. Coughlan, M. P., and F. Mayer. 1992. The cellulose-decomposing bacteria and their enzyme systems, p. 460-516. In A. Balows. H. G. Trüper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The procaryotcs, 2nd ed., vol. I. Springer Verlag, New York, N.Y.
7. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356.
8. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
9. Gerhardt, P., R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg, and G. B. Phillips. 1981. Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C.
10. Kato, S., S. Haruta, Z. J. Cui, M. Ishii, A. Yokota, and Y. Igarashi. 2004. Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community. Int. J. Syst. Evol. Microbiol. 54:2043-2047.
11. Lai, T. E., A. Nopharatana, P. C. Pullammanappallil, and W. P. Clarke. 2001. Cellulolytic activity in leachate during leach-bed anaerobic digestion of municipal solid waste. Bioresour. Technol. 80:205-210.
12. Li, T., J. G. Bisaillon, R. Villemur, L. Letourneau, K. Bernard, F. Lepine, and R. Beaudet. 1996. Isolation and characterization of a new bacterium carboxylating phenol to benzoic acid under anaerobic conditions. J. Bacteriol. 178:2551-2558.
13. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
14. Muyzer, G., E. C. de Waal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymcrasc chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.
15. Noike, T., G. Endo, J. Chang, J. Yaguchi, and J. Matsumoto. 1985. Characteristics of carbohydrate degradation and the rate-limiting step in anaerobic digestion. Biotechnol. Bioeng. 27:1482-1489.
16. Perriere, G., and M. Gouy. 1996. WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78:364-369.
17. Rainey, F. A., N. L. Ward, H. W. Morgan, R. Toalster, and E. Stackebrandt. 1993. Phylogenetic analysis of anaerobic thermophilic bacteria: aid for their reclassification. J. Bacteriol. 175:4772-4779.
18. Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
19. Syutsubo, K., Y. Nagaya, S. Sakai, and A. Miya. 2005. Behavior of cellulose-degrading bacteria in thermophilic anaerobic digestion process. Water Sci. Technol. 52:79-84.
20. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
21. Weber, S., S. Stubner, and R. Conrad. 2001. Bacterial populations colonizing and degrading rice straw in anoxic paddy soil. Appl. Environ. Microbiol. 67:1318-1327.
22. Yoon, E., and S. Jo. 2002. Municipal solid waste management in Tokyo and Seoul. Proceedings of Workshop of IGES/APN Mega-City Project, Kokura, Kitakyusyu, Japan. [Online.] http://www.iges.or.jp/cn/uc/pdf/megacity02/data /PDF/02-3(Yoon).pdf.