Molecular sorting by electrical steering of microtubules in kinesin-coated channels

Science

Volumn 312, Issue 5775, 2006, Pages 910-914

  Van Den Heuvel, Martin G L ^a   De Graaff, Martijn P ^a   Dekker, Cees ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DIMERS; ELECTRIC FIELDS; ELECTROPHORESIS; NANOSTRUCTURED MATERIALS; PHYSIOLOGY; PROTEINS;

KINESIN-COATED CHANNELS; MICROTUBULES; NANOENGINEERED STRUCTURES; SUBMICRON CHANNELS;

MOLECULAR BIOLOGY;

DIMER; KINESIN; MOLECULAR MOTOR; NANOPARTICLE; TUBULIN;

ELECTRIC FIELD; ELECTRICAL PHENOMENA; ELECTROKINESIS; PROTEIN;

ANISOTROPY; ARTICLE; CALCULATION; DIELECTRIC CONSTANT; ELECTRIC CONDUCTIVITY; ELECTRIC FIELD; ELECTROPHORETIC MOBILITY; FORCE; GEOMETRY; MATERIALS; MATHEMATICAL ANALYSIS; MICROTUBULE; MOLECULAR SORTING; NANOTECHNOLOGY; PRIORITY JOURNAL; PROCESS TECHNOLOGY; VISCOSITY;

ANISOTROPY; DIMERIZATION; ELECTRICITY; ELECTRODES; ELECTROPHORESIS; ELECTROSTATICS; KINESIN; MICROTUBULES; MOLECULAR MOTOR PROTEINS; NANOSTRUCTURES; NANOTECHNOLOGY; TUBULIN; VISCOSITY;

EID: 33646543043     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1124258     Document Type: Article

References (39)

1. R. K. Soong et al., Science 290, 1555 (2000).
2. F. Patolsky, Y. Weizmann, I. Willner, Nat. Mater. 3, 692 (2004).
3. J. Xi, J. J. Schmidt, C. D. Montemagno, Nat. Mater. 4, 180 (2005).
4. H. Hess, V. Vogel, J. Biotechnol. 82, 67 (2001).
5. M. J. Schnitzer, S. M. Block, Nature 388, 386 (1997).
6. K. J. Bohm, J. Beeg, G. M. zu Horste, R. Stracke, E. Unger, IEEE Trans. Adv. Packag. 28, 571 (2005).
7. R. Yokokawa et al., Nano Lett. 4, 2265 (2004).
8. H. Hess, G. D. Bachand, V. Vogel, Chemistry 10, 2110 (2004).
9. J. Clemmens et al., Langmuir 19, 10967 (2003).
10. S. G. Moorjani, L. Jia, T. N. Jackson, W. O. Hancock, Nano Lett. 3, 633 (2003).
11. L. Jia, S. G. Moorjani, T. N. Jackson, W. O. Hancock, Biomed. Microdevices 6, 67 (2004).
12. M. G. L. van den Heuvel, C. T. Butcher, S. G. Lemay, S. Diez, C. Dekker, Nano Lett. 5, 235 (2005).
13. Y. Hiratsuka, T. Tada, K. Oiwa, T. Kanayama, T. Q. P. Uyeda, Biophys. J. 81, 1555 (2001).
14. M. G. L. van den Heuvel, C. T. Butcher, R. M. M. Smeets, S. Diez, C. Dekker, Nano Lett. 5, 1117 (2005).
15. S. Ramachandran, K. Ernst, G. D. Bachand, V. Vogel, H. Hess, Small 2, 330 (2006).
16. R. Stracke, K. J. Bohm, L. Wollweber, J. A. Tuszynski, E. Unger, Biochem. Biophys. Res. Commun. 293, 602 (2002).
17. Materials and methods are available as supporting information on Science Online.
18. At large fields, higher than ∼110 kV/m, we observed an effect on the microtubule velocity along the electric field. Microtubules moving parallel to the electric field displayed higher and lower speeds depending on the direction of the field. For fields oriented perpendicular to the long axis, microtubules displayed sideward motion. We are investigating these effects.
19. T. Duke, T. E. Holy, S. Leibler, Phys. Rev. Lett. 74, 330 (1995).
20. D. Stigter, J. Phys. Chem. 82, 1417 (1978).
21. D. Stigter, C. Bustamante, Biophys. J. 75, 1197 (1998).
22. e|E and also equals the total force density exerted on the double layer, plus the drag force exerted on the moving counterions (21).
23. D. Stigter, Biopolymers 31, 169 (1991).
24. D = 0.8 nm for the Debye length in our 160 mM buffer.
25. A. J. Hunt, F. Gittes, J. Howard, Biophys. J. 67, 766 (1994).
26. -3 kg/ms, and h = 30 ± 10 nm for the distance of the microtubule axis to the surface.
27. F. Gittes, B. Mickey, J. Nettleton, J. Howard, J. Cell Biol. 120, 923 (1993).
28. H. Felgner, R. Frank, M. Schliwa, J. Cell Sci. 109, 509 (1996).
29. p, together with possible defects in the tip structure.
30. BT/e = 26 mV. However, at ζ = 50 mV, the use of the linearized Grahame equation introduces an error in σ of only 14%. The use of the nonlinear version of the Grahame would invoke an unknown source of error, because we would then have to assume a value for the double-layer capacitance of the microtubule.
31. E. Nogales, S. G. Wolf, K. H. Downing, Nature 393, 191 (1998).
32. P. S. Dittrich, P. Schwille, Anal. Chem. 75, 5767 (2003).
33. A. Y. Fu, C. Spence, A. Scherer, F. H. Arnold, S. R. Quake, Nat. Biotechnol. 17, 1109 (1999).
34. H. A. Stone, A. D. Stroock, A. Ajdari, Annu. Rev. Fluid Mech. 36, 381 (2004).
35. B. Song, M. Zhao, J. V. Forrester, C. D. McCaig, Proc. Natl. Acad. Sci. U.S.A. 99, 13577 (2002).
36. K. R. Robinson, L. F. Jaffe, Science 187, 70 (1975).
37. T. Nitta, H. Hess, Nano Lett. 5, 1337 (2005).
38. A. Kis et al., Phys. Rev. Lett. 89, 248101 (2002).
39. We thank J. Howard and S. Diez for kindly providing us with the kinesin expression vector and for advice with protocols; S. G. Lemay, I. Dujovne, and D. Stein for useful discussions; Y. Garini and Olympus Netherlands for lending optical equipment; and Hamamatsu Germany for kindly providing us a C7780 color camera. This work was funded by the Dutch Organization for Scientific Research (NWO) and the European Community Biomach program.