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Volumn 5, Issue 2, 2005, Pages 235-241

Electrical docking of microtubules for kinesin-driven motility in nanostructures

Author keywords

[No Author keywords available]

Indexed keywords

COATING TECHNIQUES; ELECTRIC POTENTIAL; ELECTRODES; ELECTRON BEAM LITHOGRAPHY; GOLD; REACTION KINETICS; SILICA; SWITCHING;

EID: 14644430937     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl048291n     Document Type: Article
Times cited : (52)

References (23)
  • 16
    • 14644445795 scopus 로고    scopus 로고
    • note
    • 2, 1 mM GTP, and 5% DMSO in BRB80 buffer (37°C for 60 min). Then the microtubules were stabilized and 100x diluted in BRB80 containing 10 μM Taxol (BRB80T). The final tubulin concentration would then be ∼32 nM. For the experiments in the 100 μm sized electrode, the 10 μL of polymerized microtubules were stabilized and diluted 40x in BRB80T. To remove the unpolymerized tubulin, the 400 μL was centrifuged at 178.000 g in a Beckman airfuge for 5 min and the pellet was resuspended in 1000 μL of BRB80T, yielding again a tubulin concentration of ∼32 nM. In all experiments, we shortened the microtubules by shearing them in a 30 g needle.
  • 17
    • 14644429934 scopus 로고    scopus 로고
    • note
    • q values can not, strictly speaking, be directly compared between both electrode sizes.
  • 20
    • 14644431187 scopus 로고    scopus 로고
    • note
    • diff and ruling out this possibility.
  • 21
    • 14644422847 scopus 로고    scopus 로고
    • note
    • ss are slightly modified in this control experiment. (See Supporting Information for derivations).
  • 22
    • 14644391126 scopus 로고    scopus 로고
    • note
    • 4 tubulin dimers.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.