PCR-based species-specific amplification of ITS of Mecistocirrus digitatus and its application in identification of GI nematode eggs in bovine faeces

Journal of Veterinary Medical Science

Volumn 68, Issue 4, 2006, Pages 345-351

  Mochizuki, Rieko ^a   Endoh, Daiji ^a   Onuma, Manabu ^b   Fukumoto, Shin Ichiro ^a  

^a RAKUNO GAKUEN UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

Author keywords

Cattle; Gastrointestinal nematode; ITS; Mecistocirrus digitatus; PCR

Indexed keywords

RIBOSOME DNA; SPACER DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; CATTLE; CATTLE DISEASE; CHEMISTRY; FECES; GASTROINTESTINAL DISEASE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NEMATODE; NEMATODIASIS; NUCLEOTIDE SEQUENCE; OOCYTE; PARASITOLOGY; POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE;

ANIMALS; BASE SEQUENCE; CATTLE; CATTLE DISEASES; DNA, INTERGENIC; DNA, RIBOSOMAL; FECES; GASTROINTESTINAL DISEASES; MOLECULAR SEQUENCE DATA; NEMATODA; NEMATODE INFECTIONS; OVUM; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY;

BOS TAURUS; BOVINAE; MECISTOCIRRUS; NEMATODA;

EID: 33646480184     PISSN: 09167250     EISSN: 13477439     Source Type: Journal    
DOI: 10.1292/jvms.68.345     Document Type: Article

References (25)

1. Bliss, D. H. and Todd, A. C. 1974. Milk production by Wisconsin dairy cattle after deworming with thiabendazole. Vet. Med. Sm. Anim. Clin. 69: 638-640.
2. Campbell, A. J., Gasser, R. B. and Chilton, N. B. 1995. Differences in a ribosomal DNA sequence of Strongylus species allows identification of single eggs. Int. J. Parasitol. 25: 359-365.
3. Coop, R. L. and Holms, P. H. 1996. Nutrition and parasite interaction. Int. J. Parasitol. 26: 951-962.
4. Dame, J. B., Yowell, C. A., Courtney, C. H. and Lindren, W. G. 1991. Cloning and characterization of the ribosomal RNA repeat from Ostertagia ostertagi. Mol. Biochem. Parasit. 45: 275-280.
5. Dobson, R. J., Barnes, E. H., Birclijin, S. D. and Gill, J. H. 1992. The survival of Ostertagia circumcincta and Trichostrongylus colubriformis in faecal culture as a source of bias in apportioning egg counts to worm species. Int. J. Parasitol. 22: 1005-1008.
6. Fernando, S. T. 1965. Morphology, systematics, and geographic distribution of Mecistocirrus digitatus, a Trichostrongylid parasite of ruminants. J. Parasitol. 51: 149-155.
7. Fukumoto, S., Etani, K., Toi, K., Hanadate, M., Hidaka, M., Yokoya, K., Hiramatsu, T., Iguchi, T., Kudo, S., Miyamoto, K. and Bando, G. 1990. Epidemiology of abomasal nematodes of dairy cattle in Hokkaido, Northern Japan. Jpn. J. Vet. Sci. 52: 358-379.
8. Gasbarre, L. C., Leighton, E. A. and Bryant, D. 1996. Reliability of a single fecal egg per gram determination as a measure of individual and herd values for trichostrongyle nematodes of cattle. Am. J. Vet. Res. 57: 168-171.
9. Gasser, R. B., Chilton, N. B., Hoste, H. and Beveridge, I. 1993. Rapid sequencing of rDNA from single worms and eggs of parasitic helminthes. Nucl. Acids Res. 21: 2525-2526.
10. Gasser, R. B. and Hoste, H. 1995. Genetic markers for closely-related parasitic nematodes. Mol. Cell. Probe. 9: 315-320.
11. Heise, M., Epe, C. and Schnieder, T. 1999. Differences in the second internal transcribed spacer (ITS-2) of eight species of gastrointestinal nematodes of ruminants. J. Parasitol. 85: 431-435.
12. Hoste, H., Gasser, R. B., Chilton, N. B., Mallet, S. and Beveridge, I. 1993. Lack of intraspecific variation in the second internal transcribed spacer (ITS-2) of Trichostrongylus colubriformis ribosomal DNA. Int. J. Parasitol. 23: 1069-1071.
13. Lichtenfels, J. R. and Pillitt, P. A. 2000. Synlophe patterns of the Haemonchinae of ruminants (nematode: Trichostrongyloidea). J. Parasitol. 85: 1093-1098.
14. Newton, L. A., Chilton, N. B., Beveridge, I. and Gasser, R. B. 1998. Genetic evidence indicating that Cooperia surnabada and Cooperia oncophora are one species. Int. J. Parasitol. 28: 331-336.
15. Oikawa, S., Kawai, F., Hiraga, K., Motokawa, M., Chiba, Y., Ogawa, T. and Fukumoto, S. 1998. Deworming effect of ivermectin on gastrointestinal nematodes in Holstein heifers on pasture. J. Jpn. Vet. Med. Assoc. 51: 237-240 (in Japanese with English summary).
16. Oku, Y., Nakazawa, M., Hatakeyama, S., Miyaji, S., Kitaguchi, T., Cabrera-lopez, C. A., Okamoto, M., Kamiya, M., Ohbayasi, M. and Ooi, H. 1987. A survey of abomasal and duodenal nematodes in cattle in Hokkaido, Japan. Jpn. J. Vet. Res. 35: 67-72.
17. Romstad, A., Gasser, R. B., Monti, J. R., Polderman, A. M., Nansen, P., Pit, D. S. S. and Chilton, N. B. 1997. Differentiation of Oesophagostomum bifurcum from Necator americanus by PCR using genetic markers in spacer ribosomal DNA. Mol. Cell. Probe. 11: 169-176.
18. Steel, J. W., Jones, W. O. and Symons, L. E. A. 1982. Effects of a concurrent infection of Trichostrongylus colubriformis on the productivity and physiological and metabolic responses of lambs infected with Ostertagia circumcincta. Aus. J. Agric. Res. 33: 131-140.
19. Stevenson, L. A., Chilton, N. B. and Gasser, R. B. 1995. Differentiation of Haemonchus placei from H. contortus (Nematoda: Trichostrongylidae) by the ribosomal DNA second internal transcribed spacer. Int. J. Parasitol. 25: 483-488.
20. Stevenson, L. A., Gasser, R. B. and Chilton, N. B. 1996. The ITS-2 rDNA of Teladorsagia circumcincta, T. trifurcate and T. davtiani (Nematoda: Trichostrongylidea) indicates that these taxa are one species. Int. J. Parasitol. 26: 1123-1126.
21. Van Aken, D., Vercruysse, J., Dargantes, A. P., Lagapa, J. T., Raes, S. and Shaw, D. J. 1997. Pathophysiological aspects of Mecistocirrus digitatus (Nematoda: Trichostrongylidae) infection calves. Vet. Parasitol. 69: 255-263.
22. Van Aken, D., Vercruysse, J., Dargantes, A., Valdez, L., Flores, A. and Shaw, D.J. 1998. Development of immunity of Mecistocirrus digitatus (Nematoda: Trichostrongylidae) in calves. Parasitology 117: 83-87.
23. Yasuda, N., Kawaguchi, H., Miyashita, N. and Shimizu, T. 1996. A survey of abomasal nematodes of Japanese black cattle in Kagoshima, Japan. J. Jpn. Vet. Med. Assoc. 49: 241-244 (in Japanese with English summary).
24. Zarlenga, D. S., Gasbarre, L. C., Boyd, P., Leighton, E. and Lichtenfels, J. R. 1998. Identification and semi-quantitation of Ostertagia ostertagi eggs by enzymatic amplification of ITS-1 sequences. Vet. Parasitol. 77: 245-257.
25. Zarlenga, D. S., Chute, M. B., Gasbarre, L. C. and Boyd, P. C. 2001. A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle. Vet. Parasitol. 97: 201-211.