Differentiation of Oesophagostomum bifurcum from Necator americanus by PCR using genetic markers in spacer ribosomal DNA

Molecular and Cellular Probes

Volumn 11, Issue 3, 1997, Pages 169-176

  Romstad, A ^a,c   Gasser, R B ^a   Monti, J R ^a   Polderman, A M ^b   Nansen, P ^c   Pit, D S S ^b,d   Chilton, N B ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

^d Ministry of Health   (Togo)

Author keywords

Internal transcribed spacer; Necator americanus; Oesophagostomum bifurcum; Parasitic nematodes; Ribosomal DNA; Species identification; Specific PCR

Indexed keywords

RIBOSOME DNA; SPACER DNA;

ARTICLE; EGG; FECES; GENETIC MARKER; GENETIC TRANSCRIPTION; INTESTINE; LARVA; LIFE CYCLE; NECATOR AMERICANUS; NEMATODIASIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPECIES DIFFERENCE; STRONGYLOIDEA;

ANCYLOSTOMATOIDEA; NECATOR AMERICANUS; NEMATODA; OESOPHAGOSTOMUM BIFURCUM; STRONGYLOIDEA;

EID: 0031171740     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1996.0094     Document Type: Article

References (30)

1. 1. Haaf, E. & van Soest, A. H. (1964). Oesophagostomiasis in man in North Ghana. Tropical and Geographical Medicine 16, 49-53.
2. 2. Gigase, P., Baeta, S., Kumar, V. & Brandt, J. (1987). Frequency of symptomatic human oesophagostomiasis (helminthoma) in Northern Togo. In Helminth Zoonosis (Geerts et al., eds.) Pp. 223-36. Martinus Nijhoff.
3. 3. Polderman, A. M., Krepel, H. P., Baeta, S., Blotkamp, J. & Gigase, P. (1991). Oesophagostomiasis, a common infection of man in northern Togo and Ghana. American Journal of Tropical Medicine and Hygiene 44, 336-44.
4. 4. Polderman, A. M. & Blotkamp, J. (1995). Oesophagostomum infections in humans. Parasitology Today 11, 451-6.
5. 5. Blotkamp, J., Krepel, H. P., Kumar, V., Baeta, S., van't Noorende, J. M. & Polderman, A. M. (1993). Observations on the morphology of adults and larval stages of Oesophagostomum sp. isolated from man in northern Togo and Ghana. Journal of Helminthology 67, 49-61.
6. 6. Barker, D. C. (1989). Molecular approaches to DNA diagnosis. Parasitology 99, S125-46.
7. 7. Wilson, S. M. (1991). Nucleic acid techniques and the detection of parasitic diseases. Parasitology Today 7, 255-9.
8. 8. Zarlenga, D. S. & Barta, J. R. (1990). DNA analysis in the diagnosis of infection and in the speciation of nematode parasites. Revue Scientifique et Technique d'Office International des Epizooties 9, 533-54.
9. 9. Adlard, R. D., Barker, S. C., Blair, D. & Cribb, T. H. (1993). Comparison of the second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea). International Journal for Parasitology 23, 423-5.
10. 10. Bowles, J. & McManus, D. P. (1993). Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Molecular and Biochemical Parasitology 57, 231-40.
11. 11. Morgan, J. A. T. & Blair, D. (1995). Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group. Parasitology 111, 609-15.
12. 12. Hoste, H., Chilton, N. B., Gasser, R. B. & Beveridge, I. (1995). Differences in the second internal transcribed spacer (rDNA) between five species of Trichostrongylus (Nematoda: Trichostrongylidae). International Journal for Parasitology 25, 75-80.
13. 13. Stevenson, L. A., Chilton, N. B. & Gasser, R. B. (1995). Differentiation of Haemonchus placei from H. contortus (Nematoda: Trichostrongylidae) by the ribosomal DNA second internal transcribed spacer. International Journal for Parasitology 25, 483-8.
14. 14. Stevenson, L. A., Gasser, R. B. & Chilton, N. B. (1996). The ITS2 rDNA of Teladorsagia circumcincta, T. trifurcata and T. davtiani indicates that these taxa are one species. International Journal for Parasitology 26, 1123-6.
15. 15. Campbell, A. J. D., Gasser, R. B. & Chilton, N. B. (1995). Differences in a ribosomal DNA sequence of Strongylus species allows identification of single eggs. International Journal for Parasitology 25, 359-65.
16. 16. Chabaud, A. G. & Larivière, M. (1958). Sur les oesophagostomes parasites de l'homme. Bulletin de la Societé de Pathologie Exotique 51, 384-93.
17. 17. Skrjabin, K. I., Shikhobalova, N. P., Schulz, R. S., Popova, T. I., Boev, S. N. & Delyamure, S. L. (1952). Strongylata. In Keys to Parasitic Nematodes Vol. 3 (Skrjabin, K. I., ed.) New York: E. J. Brill (English Translation; Israel Program for Scientific Translation, 1961, Jerusalem).
18. 18. Mullis, K. B., Faloona, F., Scharf, S., Saiki, R., Horn, G. & Erlich, H. (1986). Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symposium of Quantitative Biology 51, 263-73.
19. 19. Gasser, R. B., Chilton, N. B., Hoste, H. & Beveridge, I. (1993). Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Research 21, 2525-6.
20. 20. Chilton, N. B., Gasser, R. B. & Beveridge, I. (1995). Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). International Journal for Parasitology 25, 647-51.
21. 21. Gasser, R. B. & Hoste, H. (1995). Genetic markers for closely related parasitic nematodes. Molecular and Cellular Probes 9, 315-20.
22. 22. Gasser, R. B., Stevenson, L. A., Chilton, N. B., Nansen, P., Bucknell, D. G. & Beveridge, I. (1996). Species markers for equine strongyles detected in intergenic rDNA by PCR-RFLP. Molecular and Cellular Probes 10, 371-8.
23. 23. Hung, G. C., Jacobs, D. E., Krecek, R. S., Gasser, R. B. & Chilton, N. B. (1997). Strongylus asini: genetic relationships with other Strongylus species determined by ribosomal DNA. International Journal for Parasitology 26, 1407-11.
24. 24. Romstad, A., Gasser, R. B., Nansen, P., Polderman, A. M., Monti, J. R. & Chilton, N. B. (1997). Characterisation of Oesophagostomum bifurcum and Necator americanus by PCR-RFLP of rDNA. Journal of Parasitology, submitted.
25. 25. Schlötterer, C. & Tautz, D. (1994). Chromosomal homogeneity of Drosophila ribosomal DNA arrays suggests intrachromosomal exchanges drive concerted evolution. Current Biology 4, 777-83.
26. 26. Wesson, D. M., Porter, C. H. & Collins, F. H. (1992). Sequence and secondary structure comparisons of ITS rDNA in mosquitoes (Diptera: Culicidae). Molecular Phylogenetics and Evolution 1, 253-69.
27. 27. Gasser, R. B. & Chilton, N. B. (1995). Characterisation of taeniid cestode species by PCR-RFLP of ITS2 ribosomal DNA. Acta Tropica 59, 31-40.
28. 28. Gasser, R., Nansen, P. & Guldberg, P. (1996). Fingerprinting sequence variation in ribosomal DNA of parasites by DGGE. Molecular and Cellular Probes 10, 99-105.
29. 29. Scharf, S. J., Horn, G. T. & Erlich, H. A. (1986). Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233, 1076-8.
30. 30. Saiki, R. K., Gelfand, D. H., Stoffel, S. et al. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-91.