Effect of fluorine substituent on the chitinase-catalyzed polymerization of sugar oxazoline derivatives

Chemistry Letters

Volumn 35, Issue 2, 2006, Pages 160-161

  Makino, Akira ^a   Sakamoto, Junji ^a   Ohmae, Masashi ^a   Kobayashi, Shiro ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 33646456977     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2006.160     Document Type: Article

References (19)

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12. + 206.0829, found 206.0830.
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15. A typical procedure of the reaction: To a solution of 1 (0.98 mg, 4.8 μmol) in a phosphate buffer (200 μL, 10 mM, pH 7.7) was added chitinase from Bacillus sp. (0.10 mg) at 30°C. The mixture was kept standing at 30°C for 24h, then the enzyme was thermally inactivated at 90°C for 5 min. The mixture was analyzed by size exclusion chromatography (SEC) with Shodex SUGAR KS-802 column eluting with distilled water at 80°C.
16. C. Sasaki, A. Yokoyama, Y. Itoh, M. Hashimoto, T. Watanabe, T. Fukamizo, J. Biochem. 2002, 131, 557.
17. Yields of the oligosaccharides: total 35% (dimer 24%, trimer 10%, and tetramer 1%). See also: T. Kiyosada, Ph. D. Dissertation, Tohoku University (1998), Chap. 2.
18. A typical procedure of the reaction: To a solution of 2 (4.09 mg, 10 μmol) in a phosphate buffer (200 μL, 10 mM, pH 9.0) was added chitinase from Bacillus sp. (0.20 mg) at 40°C. The mixture was kept standing at 40°C for 4 h followed by thermal inactivation of the enzyme at 90°C for 5 min. A white precipitate was separated by centrifugation as a water-insoluble part of 5 (2.21 mg, 54%), and the supernatant was analyzed by HPLC to determine the yield of a water-soluble part of 5 (27%).
19. n = 5900 and 11800). MALDI-TOF/MS analysis showed 5 connected up to 10 saccharide units.