Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and enteroaggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya

Comparative Immunology, Microbiology and Infectious Diseases

Volumn 29, Issue 2-3, 2006, Pages 100-113

  Dow, M A ^a,b   Toth I ^a   Malik, A ^a   Herpay, M ^c   Nogrady N ^c   Ghenghesh, K S ^d   Nagy, B ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b Al Tahdi University   (Libyan Arab Jamahiriya)

^c Bela Johan National lnstitute of Public Health   (Hungary)

^d UNIVERSITY OF TRIPOLI   (Libyan Arab Jamahiriya)

Author keywords

Adhesion; Antibiotic resistance; Diarrhoea; EAEC; EPEC; HeLa; Integrons; Libia

Indexed keywords

AMPICILLIN; ANTIBIOTIC AGENT; CEFOTAXIME; CHLORAMPHENICOL; CIPROFLOXACIN; COTRIMOXAZOLE; GENTAMICIN; KANAMYCIN; NALIDIXIC ACID; STREPTOMYCIN; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIUM ADHERENCE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CELL CULTURE; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DIARRHEA; ENTEROAGGREGATIVE ESCHERICHIA COLI; ENTEROPATHOGENIC ESCHERICHIA COLI; GENETIC HETEROGENEITY; HELA CELL; HUMAN; HUMAN CELL; INTEGRON; LIBYAN ARAB JAMAHIRIYA; NONHUMAN; NORTH AFRICA; PATHOTYPE; PHENOTYPE; SEROTYPE; STATISTICAL ANALYSIS;

ADHESINS, BACTERIAL; AGGLUTINATION TESTS; BACTERIAL ADHESION; CHILD, PRESCHOOL; DIARRHEA; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; HELA CELLS; HUMANS; INFANT; LIBYA; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SEROTYPING; TRANS-ACTIVATORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33646348700     PISSN: 01479571     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cimid.2006.01.005     Document Type: Article

References (57)

1. Nataro J.P., and Kaper J.B. Diarrheagenic Escherichia coli. Clin Microbiol Rev 11 (1998) 142-201
2. Kaper J.B., Nataro J.P., and Mobley H.L. Pathogenic Escherichia coli. Nat Rev Microbiol 2 (2004) 123-140
3. McDaniel T.K., Jarvis K.G., Donnenberg M.S., and Kaper J.B. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci 92 (1995) 16641-16668
4. Elliott S.J., Wainwright L.A., McDaniel T.K., Jarvis K.G., Deng Y.K., Lai L.C., et al. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microbiol 28 (1998) 1-4
5. Zhang W.L., Kohler B., Oswald E., Beutin L., Karch H., Morabito S., et al. Genetic diversity of intimin genes of attaching effacing Escherichia coli strains. J Clin Microbiol 40 (2002) 4486-4492
6. Tobe T., Hayashi T., Han C.G., Schoolnik G.K., Ohtsubo E., and Sasakawa C. Complete DNA sequence structural analysis of the enteropathogenic Escherichia coli adherence factor plasmid. Infect Immun 67 (1999) 5455-5462
7. Trabulsi L.R., Keller R., and Tardelli Gomes T.A. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 8 (2002) 508-513
8. Levine M.M., Prado V., Robins-Browne R., Lior H., Kaper J.B., Moseley S.L., et al. Use of DNA probes HEp-2 cell adherence assay to detect diarrheagenic Escherichia coli. J Infect Dis 158 (1988) 224-228
9. Nataro J.P., Deng Y., Maneval D.R., German A.L., Martin W.C., and Levine M.M. Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes. Infect Immun 60 (1992) 2297-2304
10. Czeczulin J.R., Balepur S., Hicks S., Phillips A., Hall R., Kothary M.H., et al. Aggregative adherence fimbria II, a second fimbrial antigen mediating aggregative adherence in enteroaggregative Escherichia coli. Infect Immun 65 (1997) 4135-4145
11. Bernier C., Gounon P., and Le Bouguenec C. Identification of an aggregative adhesion fimbria (AAF) type III-encoding operon in enteroaggregative Escherichia coli as a sensitive probe for detecting the AAF-encoding operon family. Infect Immun 70 (2002) 4302-4311
12. Savarino S.J., Fasano A., Watson J., Martin B.M., Levine M.M., Gualini S., et al. Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another subfamily of E. coli heat-stable toxin. Proc Natl Acad Sci 90 (1993) 3093-3097
13. Eslava C., Navarro-Garcia F., Czeczulin J.R., Henderson I.R., Cravioto A., and Nataro J.P. Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli. Infect Immun 66 (1998) 3155-3163
14. Henderson I.R., Czeczulin J., Eslava C., Noriega F., and Nataro J.P. Characterization of Pic, a secreted protease of Shigella flexneri enteroaggregative Escherichia coli. Infect Immun 67 (1999) 5587-5596
15. Fasano A., Noriega F.R., Maneval Jr. D.R., Chanasongcram S., Russell R., Gualini S., et al. Shigella enterotoxin 1: an enterotoxin of Shigella flexneri 2a active in rabbit small intestine in vivo and in vitro. J Clin Invest 95 (1995) 2853-2861
16. Schubert S., Rakin A., Karch H., Carniel E., and Heesemann J. Prevalence of the 'high-pathogenicity island' of Yersinia species among Escherichia coli strains that are pathogenic to humans. Infect Immun 66 (1998) 480-485
17. Perna N.T., Plunkett G., Burland V., Mau B., Glasner J.D., Rose D.J., et al. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409 (2001) 529-533
18. Newton H.J., Sloan J., Bennett-Wood V., Adams L.M., Robins-Browne R.M., and Hartland E.L. Contribution of long polar fimbriae to the virulence of rabbit-specific enteropathogenic Escherichia coli. Infect Immun 72 (2004) 1230-1239
19. Nicholls L., Grant T.H., and Robins-Browne R.M. Identification of a novel genetic locus that is required for in vitro adhesion of a clinical isolate of enterohaemorrhagic Escherichia coli to epithelial cells. Mol Microbiol 35 (2000) 275-288
20. Klapproth J.M., Scaletsky I.C., McNamara B.P., Lai L.C., Malstrom C., James S.P., et al. A large toxin from pathogenic Escherichia coli strains that inhibits lymphocyte activation. Infect Immun 68 (2000) 2148-2155
21. Nataro J.P., Seriwatana J., Fasano A., Maneval D.R., Guers L.D., Noriega F., et al. Identification and cloning of a novel plasmid-encoded enterotoxin of enteroinvasive Escherichia coli and Shigella strains. Infect Immun 63 (1995) 4721-4728
22. Pulkkinen W.S., and Miller S.I.A. Salmonella typhimurium virulence protein is similar to a Yersinia enterocolitica invasion protein and a bacteriophage lambda outer membrane protein. J Bacteriol 173 (1991) 86-93
23. Karmali M.A., Mascarenhas M., Shen S., Ziebell K., Johnson S., Reid-Smith R., et al. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. J Clin Microbiol 41 (2003) 4930-4940
24. Torres M.E., Pirez M.C., Schelotto F., Varela G., Parodi V., Allende F., et al. Etiology of children's diarrhea in Montevideo, Uruguay: associated pathogens unusual isolates. J Clin Microbiol 39 (2001) 2134-2139
25. Abu-Elyazeed R., Wierzba T.F., Mourad A.S., Peruski L.F., Kay B.A., Rao M., et al. Epidemiology of enterotoxigenic Escherichia coli diarrhea in a pediatric cohort in a periurban area of lower, Egypt. J Infect Dis 179 (1999) 382-389
26. Shaheen H.I., Khalil S.B., Rao M.R., Abu Elyazeed R., Wierzba T.F., Peruski Jr. L.F., et al. Phenotypic profiles of enterotoxigenic Escherichia coli associated with early childhood diarrhea in rural Egypt. J Clin Microbiol 42 (2004) 5588-5595
27. Ghenghesh K.S., Bara F., Bukris B., el-Surmani A., and Abeid S.S. Characterization of virulence factors of Aeromonas isolated from children with and without diarrhoea in Tripoli, Libya. J Diarrhoeal Dis Res 17 (1999) 75-80
28. Orskov L., and Orskov F. Serotyping of Escherichia coli. In: Bergan T., and Norris J.R. (Eds). Methods in microbiology (1984), Academic Press, New York 43-112
29. Cravioto A., Gross R.J., Scotl S.M., and Rowe B. An adhesive factor found in strains of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Curr Microbiol 3 (1979) 95-99
30. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing; Twelfth Informational Supplement. Document M100-S11. Wayne, PA: NCCLS; 2002.
31. China B., Pirson V., and Mainil J. Typing of bovine attaching effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl Environ Microbiol 62 (1996) 3462-3465
32. Oswald E., Schmidt H., Morabito S., Karch H., Marchés O., and Caprioli A. Typing of intimin genes in human animal enterohemorrhagic enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect Immun 68 (2000) 64-71
33. Franke S., Schmidt H., Schwarzkopf A., Wieler L.H., Baljer G., Beutin L., et al. Nucleotide sequence analysis of enteropathogenic Escherichia coli (EPEC) adherence factor probe development of PCR for rapid detection of EPEC harboring virulence plasmids. J Clin Microbiol 32 (1994) 2460-2463
34. Grunzburg S.T., Tornieporth N.G., and Riley L.W. Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene. J Clin Microbiol 33 (1995) 1375-1377
35. Batisson I., Guimond M.P., Girard F., An H., Zhu C., Oswald E., et al. Characterization of the novel factor paa involved in the early steps of the adhesion mechanism of attaching effacing Escherichia coli. Infect Immun 71 (2003) 4516-4525
36. Yatsuyanagi J., Saito S., Sato H., Miyajima Y., Amano K., and Enomoto K. Characterization of enteropathogenic enteroaggregative Escherichia coli isolated from diarrheal outbreaks. J Clin Microbiol 40 (2002) 294-297
37. Schmidt H., Knop C., Franke S., Aleksic S., Heesemann J., and Karch H. Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol 33 (1995) 701-705
38. Czeczulin J.R., Whittam T.S., Henderson I.R., Navarro-Garcia F., and Nataro J.P. Phylogenetic analysis of enteroaggregative diffusely adherent Escherichia coli. Infect Immun 67 (1999) 2692-2699
39. Elias W.P., Suzart S., Trabulsi L.R., Nataro J.P., and Gomes T.A. Distribution of aggA and aafA gene sequences among Escherichia coli isolates with genotypic or phenotypic characteristics, or both, of enteroaggregative E. coli. J Med Microbiol 48 (1999) 597-599
40. Piva I.C., Pereira A.L., Ferraz L.R., Silva R.S.N., Vieira A.C., Blanco J.E., et al. Virulence markers of enteroaggregative Escherichia coli isolated from children adults with diarrhea in Brasilia, Brazil. Infect Immun 41 (2003) 1827-1832
41. Blanco M., Blanco J.E., Blanco J., Alonso M.P., Balsalobre C., Mourinó M., et al. Polymerase chain reaction for detection of Escherichia coli strains producing cytotoxic necrotizing factor type 1 and type 2. J Microbiol Methods 26 (1996) 95-101
42. Tóth I., Herault F., Beutin L., and Oswald E. Production of cytolethal distending toxins by pathogenic Escherichia coli strains isolated from human animal sources: establishment of the existence of a new cdt variant (Type IV). J Clin Microbiol 41 (2003) 4285-4291
43. Doughty S., Sloan J., Bennett-Wood V., Robertson M., Robins-Browne R.M., and Hartland E.L. Identification of a novel fimbrial gene cluster related to long polar fimbriae in locus of enterocyte effacement-negative strains of enterohemorrhagic Escherichia coli. Infect Immun 70 (2002) 6761-6769
44. Lévesque C., Piché L., Larose C., and Roy P.H. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 39 (1995) 185-191
45. Sethabuter O., Venkatesan M., Murphy G.S., Eampokalap B., Hoge C.W., and Echeverria P. Detection of Shigellae enteroinvasive and Escherichia coli by amplification of the invasion plasmid antigen H DNA sequence in patients with dysentery. J Infect Dis 167 (1993) 458-461
46. Alexa P., Rychlík I., Nejezchleb A., and Hamrík J. Identification of enterotoxin-producing strains of Escherichia coli by PCR biological methods. Vet Med 42 (1997) 97-100
47. Lin Z., Kurazono H., Yamasaki S., and Takeda Y. Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol Immunol 37 (1993) 543-548
48. Sarantuya J., Nishi J., Wakimoto N., Erdene S., Nataro J.P., Sheikh J., et al. Typical enteroaggregative Escherichia coli is the most prevalent pathotype among E. coli strains causing diarrhea in Mongolian children. J Clin Microbiol 42 (2004) 133-139
49. Scaletsky I.C., Fabbricotti S.H., Silva S.O., Morais M.B., and Fagundes-Neto U. HEp-2-adherent Escherichia coli strains associated with acute infantile diarrhea, Sao Paulo, Brazil. Emerg Infect Dis 8 (2002) 855-858
50. Albert M.J., Faruque S.M., Faruque A.S., Neogi P.K., Ansaruzzaman M., Bhuiyan N.A., et al. Controlled study of Escherichia coli diarrheal infections in Bangladeshi children. J Clin Microbiol 33 (1995) 973-977
51. Okeke I.N., Lamikanra A., Czeczulin J., Dubovsky F., Kaper J.B., and Nataro J.P. Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in Southwest Nigeria. J Infect Dis 181 (2000) 252-260
52. Bhan M.K., Raj P., Levine M.M., Kaper J.B., Bhari N., Srivastava R., et al. Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J Infect Dis 159 (1989) 1061-1064
53. Bouzari S., Jafari A., Azizi A., Oloomi M., and Nataro J.P. Short report: characterization of enteroaggregative Escherichia coli isolates from Iranian children. Am J Trop Med Hyg 65 (2001) 13-14
54. Huppertz H.I., Rutkowski S., Aleksic S., and Karch H. Acute and chronic diarrhoea and abdominal colic associated with enteroaggregative Escherichia coli in young children living in Western Europe. Lancet 349 (1997) 1660-1662
55. Pabst W.L., Altwegg M., Kind C., Mirjanic S., Hardegger D., and Nadal D. Prevalence of enteroaggregative Escherichia coli among children with and without diarrhea in Switzerland. J Clin Microbiol 41 (2003) 2289-2293
56. Zamboni A., Fabbricotti S.H., Fagundes-Neto U., and Scaletsky I.C. Enteroaggregative Escherichia coli virulence factors are found to be associated with infantile diarrhea in Brazil. J Clin Microbiol 42 (2004) 1058-1063
57. Gassama A., Aidara-Kane A., Chainier D., Denis F., and Ploy M.C. Integron-associated antibiotic resistance in enteroaggregative and enteroinvasive Escherichia coli. Microb Drug Resist 10 (2004) 27-30