Investigations of cell immobilization in alginate: Rheological and electrostatic extrusion studies

Journal of Chemical Technology and Biotechnology

Volumn 81, Issue 4, 2006, Pages 505-510

  Manojlovic, Verica ^a   Djonlagic, Jasna ^a   Obradovic, Bojana ^a   Nedovic, Viktor ^a   Bugarski, Branko ^a  

^a UNIVERSITY OF BELGRADE   (Serbia)

Author keywords

Alginate; Cell immobilization; Electrostatic extrusion; Rheology

Indexed keywords

COLLOIDS; CROSSLINKING; ELECTROSTATICS; EXTRUSION; GELATION; HYDROGELS; RHEOLOGY; VISCOSITY; YEAST;

ALGINATE; ELECTROSTATIC EXTRUSION; YEAST CELLS;

CELL IMMOBILIZATION;

ALGINIC ACID; CALCIUM ION; GLUCURONIC ACID;

CELL IMMOBILIZATION; COLLOIDS; CROSSLINKING; ELECTROSTATICS; EXTRUSION; GELATION; HYDROGELS; RHEOLOGY; VISCOSITY; YEAST;

ARTICLE; CELL ACTIVITY; CELL COUNT; CELL SUSPENSION; CELL TRANSPORT; CELL TYPE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CROSS LINKING; ELECTRICITY; FLOW KINETICS; GELATION; HYDROGEL; IMMOBILIZED CELL; MOLECULAR SIZE; NONHUMAN; PLASTICITY; STATISTICAL ANALYSIS; VISCOSITY; YEAST CELL;

EID: 33646205685     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1465     Document Type: Article

References (16)

1. Murano E, Natural gelling polysaccharides: indispensable partners in bioencapsulation technology. Minerva Biotec 12:213-222 (2000).
2. Stand BL, Morch YA and Skjak-Braek G, Alginate as immobilization matrix for cells. Minerva Biotec 12:223-233 (2000).
3. Martinsen A, Skjak-Braek G and Smidsrod O, Alginate as immobilization material; I. Correlation between chemical and physical properties of alginate gel beads. Biotechnol Bioeng 33:79-89 (1989).
4. Gombotz WR and Wee SF, Protein release from alginate matrices. Adv Drug Delivery Rev 31:267-285 (1998).
5. Moe ST, Draget KI, Skjak-Braek G and Smidsrod O, Alginates, in Food Polysaccharides and Their Applications, ed by Stephen AM. Marcel Dekker, New York, Vol. 67, pp 245-286 (1995).
6. Smidsrod O and Skjak-Braek G, Alginate as immobilization matrix for cells. Trends Biotechnol 8:71-78 (1990).
7. Eiselt P, Yeh J, Latvala RK, Shea LD and Mooney DJ, Porous carriers for biomedical applications based on alginate hydrogels. Biomaterials 21:1921-1927 (2000).
8. Keshavartz T, Ramsden G, Phillips P, Mussenden P and Bucke C, Application of electric field for production of immobilized biocatalysts. Biotechnol Tech 6:445-450 (1992).
9. Bugarski B, Li Q, Goosen MFA, Poncelet D, Neufeld R and Vunjak G, Electrostatic droplet generation: mechanism of polymer droplet formation. AlChE J 40:1026-1032 (1994).
10. Manojlovic V, Obradovic B, Nedovic V and Bugarski B, Electrostatic generation of alginate microbeads loaded with brewing yeast. Chemical Industry 58:62-64 (2004).
11. Poncelet D, Babak V, Neufeld RJ, Goosen M and Bugarski B, Theory of electrostatic dispersion of polymer solution in the production of microgel beds containing biocatalyst. Advances in Colloid Interface Science 79:213-228 (1999).
12. Gilson CD, Thomas A and Hawkes FR, Gelling mechanism of alginate beads with and without immunobilized yeast. Process Biochemistry 25:104-108 (1990).
13. Jayasinghe SN and Edirisinge MJ, Electrically forced jets and microthreads of high viscosity dielectric liquids. Aerosol Science 35:233-243 (2004).
14. Bugarski B, Obradovic B, Nedovic VA and Poncelet D, Immobilization of cells and enzymes using electrostatic droplet generator, in Focus on Biotechnology, Vol. 8a: Fundamentals of Cell Immobilisation Biotechnology, ed by Nedovic V and Willaert RG. Kluwer Academic Publishers, Dordrecht, pp 277-294 (2004).
15. Bugarski B, Obradovic B, Nedovic VA and Goosen MFA, Electrostatic droplet generation technique for cell immobilization, in Finely Dispersed Particles: Micro-, Nano- and Atto-Engineering, ed by Hsu JP and Spasic A. CRC Press, Boca Raton pp 869-886 (2005). (in press).
16. Camelin I, Lacroix C, Paquin C, Prevost H, Cachon R and Divies C, Effect of chelatants on gellan gel rheological properties and setting temperature for immobilzitaion of living Bifidobacteria. Biotechnol Prog 9:291-297 (1993).