Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells

Biophysical Journal

Volumn 90, Issue 1, 2006, Pages 357-365

  Lo, Chien Jung ^a   Leake, Mark C ^a   Berry, Richard M ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGEN; PROTON; SODIUM; SODIUM ION;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CONTROLLED STUDY; ESCHERICHIA COLI; FLAGELLUM; FLUORESCENCE; HOMEOSTASIS; NONHUMAN; SODIUM CELL LEVEL; SODIUM TRANSPORT; STATISTICAL SIGNIFICANCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33646163009     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.105.071332     Document Type: Article

References (27)

1. Berry, R. M., and J. P. Armitage. 1999. The bacterial flagella motor. Adv. Microbiol. Physiol. 41:291-337.
2. Berg, H. C. 2003. The rotary motor of bacterial flagella. Annu. Rev. Biochem. 72:19-54.
3. Blair, D. F., and H. C. Berg. 1990. The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell. 60:439-449.
4. Shioi, J.-I., S. Matsuura, and T. Imae. 1980. Quantitative measurement of proton motive force and motility in Bacillus subtilis. J. Bacteriol. 144:891-897.
5. +-driven flagellar motor of Vibrio. Biochim. Biophys. Acta. 1505:82-93.
6. McCarter, L. L. 2001. Polar flagella motility of the Vibrionaceae. Microbiol. Mol. Biol. Rev. 65:445-462.
7. Blair, D. F. 2003. Flagellar movement driven by proton translocation. FEBS Lett. 545:86-95.
8. Ryu, W. S., R. M. Berry, and H. C. Berg. 2000. Torque-generating units of the flagellar motor of Escherichia coli have a high duty ratio. Nature. 403:444-447.
9. +-driven flagellar motors. J. Mol. Biol. 327:453-463.
10. Epstein, W., and S. G. Schultz. 1965. Cation transport in E. coli. V. Regulation of cation content. J. Gen. Physiol. 49:221-234.
11. Reenstra, W. W., L. Patel, H. Rottenberg, and H. R. Kaback. 1980. Electrochemical proton gradient in inverted membrane vesicles from Escherichia coli. Biochemistry. 19:1-9.
12. + in the halotolerant bacterium Brevibacterium sp. and Escherichia coli. Microbiology. 140:729-736.
13. 23Na nuclear magnetic resonance. J. Biol. Chem. (Tokyo). 261:3288-3294.
14. + measurement using Sodium Green tetraacetate with flow cytometry. Cytometry. 21:248-256.
15. Rodriguez, E., and A. Darszon. 2003. Intracellular sodium changes during the speract response and the acrosome reaction of sea urchin sperm. J. Physiol. 546:89-100.
16. Molecular Probes. The Handbook. http://probes.invitrogen.com/handbook/
17. Rowe, A. D., M. C. Leake, H. Morgan, and R. M. Berry. 2003. Rapid rotation of micron and submicron dielectric particles measured using optical tweezers. J. Mod. Opt. 50:1539-1554.
18. Sowa, Y., A. D. Rowe, M. C. Leake, T. Yakushi, M. Homma, A. Ishijima, and R. M. Berry. 2005. Direct observation of steps in rotation of the bacterial flagellar motor. Nature. 437:916-919.
19. Meister, M., G. Lowe, and H. C. Berg. 1987. The proton flux through the bacterial flagellar motor. Cell. 49:643-650.
20. Häse, C. C., N. D. Fedorova, M. Y. Galperin, and P. A. Dibrov. 2001. Sodium ion cycle in bacterial pathogens: evidence from cross-genome comparisons. Microbiol. Mol. Biol. Rev. 65:353-370.
21. Turner, L., W. S. Ryu, and H. C. Berg. 2000. Real-time imaging of fluorescent flagellar filaments. J. Bacteriol. 182:2793-2801.
22. +-driven flagellar motor of an Alkalophlic Bacillus strain YN-1. J. Biol. Chem. (Tokyo). 258:10577-10581.
23. Liu, J. Z., M. Dapice, and S. Khan. 1990. Ion selectivity of the Vibrio alginolyticus flagellar motor. J. Bacteriol. 172:5236-5244.
24. +-driven flagellar motor of Vibrio alginolyticus. J. Mol. Biol. 327:1043-1051.
25. Gabel, C. V., and H. C. Berg. 2003. The speed of the flagellar rotary motor of Escherichia coli varies linearly with protonmotive force. Proc. Natl. Acad. Sci. USA. 100:8748-8751.
26. Felle, H., J. S. Porter, C. L. Slayman, and H. R. Kaback. Quantitative measurements of membrane potential in Escherichia coli. Biochemistry. 19:3585-3590.
27. 31P nuclear magnetic resonance. J. Biol. Chem. (Tokyo). 261:7797-7806.