Torque-generating units of the flagellar motor of Escherichia coli have a high duty ratio

Nature

Volumn 403, Issue 6768, 2000, Pages 444-447

  Ryu, William S ^a,b   Berry, Richard M ^c   Berg, Howard C ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; KINESIN; MOTA PROTEIN; MYOSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ESCHERICHIA COLI; FLAGELLUM; MICROTUBULE; MOTOR ACTIVITY; MUSCLE CONTRACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TORQUE;

BACTERIAL PROTEINS; ESCHERICHIA COLI; FLAGELLA; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; MOLECULAR MOTOR PROTEINS; TORQUE;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034719374     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35000233     Document Type: Article

References (30)

1. Block, S. M. & Berg, H. C. Successive incorporation of force-generating units in the bacterial rotary motor. Nature 309, 470-473 (1984).
2. Blair, D. F. & Berg, H. C. Restoration of torque in defective flagellar motors. Science 242, 1678-1681 (1988).
3. Samuel, A. D. T. & Berg, H. C. Torque-generating units of the bacterial flagellar motor step independently. Biophys. J. 71, 918-923 (1996).
4. Leibler, S. & Huse, D. A. Porters versus rowers: a unified stochastic model of motor proteins. J. Cell Biol. 121, 1357-1368 (1993).
5. Macnab, R. M. in Escherichia coli and Salmonella; Cellular and Molecular Biology Vol. 1 (eds Neidhardt, F. C. et al.) 123-145 (American Society for Microbiology, Washington, 1996).
6. Berry, R. M. & Armitage, J. P. The bacterial flagellar motor. Adv. Microb. Physiol. 41, 291-337 (1999).
7. Silverman, M. & Simon, M. Flagellar rotation and the mechanism of bacterial motility. Nature 249, 73-74 (1974).
8. Gittes, F. & Schmidt, C. F. Interference model for back-focal-plane displacement detection in optical tweezers. Opt. Lett. 23, 7-9 (1998).
9. Berg, H. C. & Turner, L. Torque generated by the flagellar motor of Escherichia coli. Biophys. J. 65, 2201-2216 (1993).
10. Chen, X. & Berg, H. C. Torque-speed relationship of the flagellar rotary motor of Escherichia coli. Biophys. J. (in the press).
11. Block, S. M., Goldstein, L. S. B. & Schnapp, B. J. Bead movement by single kinesin molecules studied with optical tweezers. Nature 348, 348-352 (1990).
12. Uyeda, T., Abramson, P. D. & Spudich, J. A. The neck region of the myosin motor domain acts as a lever arm to generate movement. Proc. Natl Acad. Sci. USA 93, 4459-4464 (1996).
13. Howard, J. Molecular motors: structural adaptations to cellular functions. Nature 389, 561-567 (1997).
14. Macnab, R. M. Bacterial flagella rotating in bundles: a study in helical geometry. Proc. Natl Acad. Sci. USA 74, 221-225 (1997).
15. DeRosier, D. J. The turn of the screw: the bacterial flagellar motor. Cell 93, 17-20 (1998).
16. Muramoto, K. et al. High-speed rotation and speed stability of the sodium-driven flagellar motor in Vibrio alginolyticus. J. Mol. Biol. 251, 50-58 (1995).
17. Visscher, K., Schnitzer, M. J. & Block, S. M. Single kinesin molecules studied with a molecular force clamp. Nature 400, 184-189 (1999).
18. Toyoshima, Y. Y. et al. Myosin subfragment-1 is sufficient to move actin filaments in vitro. Nature 328, 536-539 (1987).
19. 1-ATPase is a highly efficient molecular motor that rotates with discrete 120° steps. Cell 93, 1117-1124 (1998).
20. Silverman, M. & Simon, M. Operon controlling motility and chemotaxis in E. coli. Nature 264, 577-580 (1976).
21. Armstrong, J. B. & Adler, J. Genetics of motility in Escherichia coli: complementation of paralyzed mutants. Genetics 56, 363-373 (1967).
22. Fung, D. C. Y. K. Powering the Escherichia coli Flagellar Motor with an External Voltage Source. Thesis, Harvard Univ. (1994).
23. Maurer, L. & Orndorff, P. E. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pill. J. Bacteriol. 169, 640-645 (1987).
24. Kuwajima, G. Flagellin domain that affects H antigenicity of Escherichia coli K-12. J. Bacteriol. 170, 485-488 (1988).
25. Scharf, B. E., Fahrner, K. A., Turner, L. & Berg, H. C. Control of direction of flagellar rotation in bacterial chemotaxis. Proc. Natl Acad. Sci. USA 95, 201-206 (1998).
26. Berry, R. M. & Berg, H. C. Absence of a barrier to backwards rotation of the bacterial flagellar motor demonstrated with optical tweezers. Proc. Natl Acad. Sci. USA 94, 14433-14437 (1997).
27. Happel, J. & Brenner, H. Low Reynolds Number Hydrodynamics (Kluwer Academic, Drodrecht, 1991).
28. Berry, R. M. & Berg, H. C. Torque generated by the flagellar motor of Escherichia coli while driven backward. Biophys. J. 76, 580-587 (1999).
29. Block, S. M., Blair, D. F. & Berg, H. C. Compliance of bacterial flagella measured with optical tweezers. Nature 338, 514-517 (1989).
30. Block, S. M., Blair, D. F. & Berg, H. C. Compliance of bacterial polyhooks measured with optical tweezers. Cytometry 12, 492-496 (1991).