Produce isolates of the Escherichia coli Ont:H52 serotype that carry both Shiga toxin 1 and stable toxin genes

Applied and Environmental Microbiology

Volumn 72, Issue 4, 2006, Pages 3062-3065

  Monday, Steven R ^a   Keys, Christina ^a   Hanson, Patricia ^b   Shen, Yuelian ^b   Whittam, Thomas S ^c   Feng, Peter ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b FLORIDA DEPARTMENT OF AGRICULTURE AND CONSUMER SERVICES   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; GENES; NUCLEIC ACIDS; STRAIN; TOXIC MATERIALS;

CLONAL GROUP; ONT:H52; POLYMORPHISM; SHIGA TOXIN 1;

ESCHERICHIA COLI;

TOXIN; VEROTOXIN 1;

COLIFORM BACTERIUM; ELECTROKINESIS; PHENOTYPE; TOXIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CLONE; CONTROLLED STUDY; ESCHERICHIA COLI; FOOD CONTAMINATION; GENE EXPRESSION; GENETIC POLYMORPHISM; GENOTYPE; NONHUMAN; PHENOTYPE; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; SEROTYPE; STX1 GENE;

BACTERIAL TOXINS; BACTERIAL TYPING TECHNIQUES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEMOLYSIN PROTEINS; SEROTYPING; SHIGA-LIKE TOXIN I; VEGETABLES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33646105166     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.72.4.3062-3065.2006     Document Type: Article

References (18)

1. Centers for Disease Control and Prevention. 1998. Standardized molecular subtyping of food borne bacterial pathogens by pulsed-field gel electrophoresis: CDC training manual. Food Borne and Diarrheal Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Ga.
2. Don, R. H., P. T. Cox, B. J. Wainwright, K. Baker, and J. S. Mattick. 1991. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:4008.
3. Donnenberg, M. S., and T. S. Whittam. 2001. Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli. J. Clin. Investig. 107:539-548.
4. Feng, P., M. Dey, A. Abe, and T. Takeda. 2001. Isogenic strain of Escherichia coli O157:H7 that has lost both Shiga toxin 1 and 2 genes. Clin. Diagn. Lab. Immunol. 8:711-717.
5. Feng, P., K. A. Lampel, H. Karch, and T. S. Whittam. 1998. Sequential genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. J. Infect. Dis. 177:1750-1753.
6. Feng, P., and S. R. Monday. 2000. Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes. Mol. Cell. Probes 14:333-337.
7. Fukushima, H., K. Hoshina, and M. Gomyoda. 2000. Selective isolation of eae-positive strains of Shiga toxin-producing Escherichia coli. J. Clin. Microbiol. 38:1684-1687.
8. Karch, H., T. Meyer, H. Russmann, and J. Heesemann. 1992. Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation. Infect. Immun. 60:3464-3467.
9. Large, T. M., S. T. Walk, and T. S. Whittam. 2005. Variation in acid resistance among Shiga toxin-producing clones of pathogenic Escherichia coli. Appl. Environ. Microbiol. 71:2493-2500.
10. Miyao, Y., T. Kataoka, T. Nomoto, A. Kai, T. Itoh, and K. Itoh. 1998. Prevalence of verotoxin-producing Escherichia coli harbored in the intestine of cattle in Japan. Vet. Microbiol. 61:137-143.
11. Muniesa, M., and J. Jofre. 1998. Abundance in sewage of bacteriophages that infect Escherichia coli O157:H7 and that carry the Shiga toxin 2 gene. Appl. Environ. Microbiol. 64:2443-2448.
12. Paton, A. W., and J. C. Paton. 1996. Enterobacter cloacae producing a Shiga-like toxin II-related cytotoxin associated with a case of hemolytic-uremic syndrome. J. Clin. Microbiol. 34:463-465.
13. Qi, W., D. W. Lacher, A. C. Bumbaugh, K. E. Hyma, L. M. Ouellette, T. M. Large, C. L. Tarr, and T. S. Whittam. 2004. EcMLST: an online database for multi locus sequence typing of pathogenic Escherichia coli, p. 520-521. Proceedings of the IEEE 2004 Computational Systems Bioinformatics Conference. IEEE Computer Society Press, Los Alamitos, Calif.
14. Reid, S. D., D. J. Betting, and T. S. Whittam. 1999. Molecular identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR. J. Clin. Microbiol. 37:2719-2722.
15. Sivapalasingam, S., C. R. Friedman, L. Cohen, and R. V. Tauxe. 2004. Fresh produce: a growing cause of outbreaks of food borne illness in the United States, 1973 through 1997. J. Food Prot. 67:2342-2353.
16. Tschape, H., R. Prager, W. Streckel, A. Fruth, E. Tietze, and G. Bohme. 1995. Verocytotoxigenic Citrobacter freundii associated with severe gastroenteritis and cases of haemolytic uraemic syndrome in a nursery school: green butter as the infection source. Epidemiol. Infect. 114:441-450.
17. Whittam, T. S. 1998. Evolution of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains, p. 195-209. In J. B. Kaper and A. D. O'Brien (ed.), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. American Society for Microbiology, Washington, D.C.
18. Wolf, M. K. 1997. Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin. Microbiol. Rev. 10:569-584.