메뉴 건너뛰기




Volumn 39, Issue 4, 2006, Pages 373-377

Preanalytical aspects of quantitative TaqMan real-time RT-PCR: Applications for TF and VEGF mRNA quantification

Author keywords

Agilent bioanalyzer; Human blood; mRNA; Real time PCR; RNA isolation; RNA quantity; Tissue factor; Vascular endothelial growth factor

Indexed keywords

EDETIC ACID; MESSENGER RNA; THROMBOPLASTIN; VASCULOTROPIN;

EID: 33646095760     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2006.02.003     Document Type: Article
Times cited : (8)

References (20)
  • 1
    • 0036310275 scopus 로고    scopus 로고
    • Molecular profiling of angiogenesis markers
    • Shih S.C., Robinson G.S., Perruzzi C.A., et al. Molecular profiling of angiogenesis markers. Am. J. Pathol. 161 (2002) 35-41
    • (2002) Am. J. Pathol. , vol.161 , pp. 35-41
    • Shih, S.C.1    Robinson, G.S.2    Perruzzi, C.A.3
  • 2
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream
    • Ginzinger D.G. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp. Hematol. 30 (2002) 503-512
    • (2002) Exp. Hematol. , vol.30 , pp. 503-512
    • Ginzinger, D.G.1
  • 3
    • 0035710229 scopus 로고    scopus 로고
    • An overview of real-time quantitative PCR: applications to quantify cytokine gene expression
    • Giulietti A., Overbergh L., Valckx D., Decallonne B., Bouillon R., and Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods 25 (2001) 386-401
    • (2001) Methods , vol.25 , pp. 386-401
    • Giulietti, A.1    Overbergh, L.2    Valckx, D.3    Decallonne, B.4    Bouillon, R.5    Mathieu, C.6
  • 4
    • 0036281212 scopus 로고    scopus 로고
    • Matthews JL, Chung M, Matyas RJ, Persistent DNA contamination in competitive RT PCR using cRNA internal standards: identity, quantity, and control. BioTechniques 32 (2002) 1412-4, 6-7.
  • 5
    • 1242296154 scopus 로고    scopus 로고
    • Inhibition of real-time RT PCR quantification due to tissue specific contaminants
    • Tichopad A., Didier A., and Pfaffl M.W. Inhibition of real-time RT PCR quantification due to tissue specific contaminants. Mol. Cell. Probes 18 (2004) 45-50
    • (2004) Mol. Cell. Probes , vol.18 , pp. 45-50
    • Tichopad, A.1    Didier, A.2    Pfaffl, M.W.3
  • 6
    • 0036018466 scopus 로고    scopus 로고
    • A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction
    • Fronhoffs S., Totzke G., Stier S., et al. A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction. Mol. Cell. Probes 16 (2002) 99-110
    • (2002) Mol. Cell. Probes , vol.16 , pp. 99-110
    • Fronhoffs, S.1    Totzke, G.2    Stier, S.3
  • 7
    • 0037371505 scopus 로고    scopus 로고
    • PCR based calibration curves for studies of quantitative gene expression in human monocytes: development and evaluation
    • Øvstebø R., Haug K.B., Lande K., and Kierulf P. PCR based calibration curves for studies of quantitative gene expression in human monocytes: development and evaluation. Clin. Chem. 49 (2003) 425-432
    • (2003) Clin. Chem. , vol.49 , pp. 425-432
    • Øvstebø, R.1    Haug, K.B.2    Lande, K.3    Kierulf, P.4
  • 8
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT PCR
    • Pfaffl M.W. A new mathematical model for relative quantification in real-time RT PCR. Nucleic Acids Res. 29 (2001) e45
    • (2001) Nucleic Acids Res. , vol.29
    • Pfaffl, M.W.1
  • 9
    • 0036581160 scopus 로고    scopus 로고
    • Relative expression software tool (REST) for group wise comparison and statistical analysis of relative expression results in real-time PCR
    • Pfaffl M.W., Horgan G.W., and Dempfle L. Relative expression software tool (REST) for group wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30 (2002) e36
    • (2002) Nucleic Acids Res. , vol.30
    • Pfaffl, M.W.1    Horgan, G.W.2    Dempfle, L.3
  • 10
    • 0037109753 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    • Tricarico C., Pinzani P., Bianchi S., et al. Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309 (2002) 293-300
    • (2002) Anal. Biochem. , vol.309 , pp. 293-300
    • Tricarico, C.1    Pinzani, P.2    Bianchi, S.3
  • 11
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper Excel based tool using pair wise correlations
    • Pfaffl M.W., Tichopad A., Prgomet C., and Neuvians T.P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper Excel based tool using pair wise correlations. Biotechnol. Lett. 26 (2004) 509-515
    • (2004) Biotechnol. Lett. , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 12
  • 13
    • 18644367266 scopus 로고    scopus 로고
    • Stabilization of mRNA expression in whole blood samples
    • Rainen L., Oelmueller U., Jurgensen S., et al. Stabilization of mRNA expression in whole blood samples. Clin. Chem. 48 (2002) 1883-1890
    • (2002) Clin. Chem. , vol.48 , pp. 1883-1890
    • Rainen, L.1    Oelmueller, U.2    Jurgensen, S.3
  • 14
    • 0030895171 scopus 로고    scopus 로고
    • Wilfinger WW, Mackey K, Chomczynski P, Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. BioTechniques 22 (1997) 474-6, 8-81.
  • 15
    • 0036320413 scopus 로고    scopus 로고
    • Real-time PCR technology for cancer diagnostics
    • Bernard P.S., and Wittwer C.T. Real-time PCR technology for cancer diagnostics. Clin. Chem. 48 (2002) 1178-1185
    • (2002) Clin. Chem. , vol.48 , pp. 1178-1185
    • Bernard, P.S.1    Wittwer, C.T.2
  • 17
    • 13444291033 scopus 로고    scopus 로고
    • Serum vascular endothelial growth factor (VEGF) and VEGF C levels as tumor markers in patients with cervical carcinoma
    • Mitsuhashi A., Suzuka K., Yamazawa K., Matsui H., Seki K., and Sekiya S. Serum vascular endothelial growth factor (VEGF) and VEGF C levels as tumor markers in patients with cervical carcinoma. Cancer 103 (2005) 724-730
    • (2005) Cancer , vol.103 , pp. 724-730
    • Mitsuhashi, A.1    Suzuka, K.2    Yamazawa, K.3    Matsui, H.4    Seki, K.5    Sekiya, S.6
  • 18
    • 1642341137 scopus 로고    scopus 로고
    • VEGF and HIF 1alpha expression are increased in advanced stages of epithelial ovarian cancer
    • Wong C., Wellman T.L., and Lounsbury K.M. VEGF and HIF 1alpha expression are increased in advanced stages of epithelial ovarian cancer. Gynecol. Oncol. 91 (2003) 513-517
    • (2003) Gynecol. Oncol. , vol.91 , pp. 513-517
    • Wong, C.1    Wellman, T.L.2    Lounsbury, K.M.3
  • 19
    • 1642462383 scopus 로고    scopus 로고
    • Measurement of tissue factor messenger RNA levels in leukocytes from patients in hypercoagulable state caused by several underlying diseases
    • Sase T., Wada H., Nishioka J., et al. Measurement of tissue factor messenger RNA levels in leukocytes from patients in hypercoagulable state caused by several underlying diseases. Thromb. Haemost. 89 (2003) 660-665
    • (2003) Thromb. Haemost. , vol.89 , pp. 660-665
    • Sase, T.1    Wada, H.2    Nishioka, J.3
  • 20
    • 0028807490 scopus 로고
    • Improved RNase protection assay for quantifying LDL receptor mRNA; estimation of analytical imprecision and biological variance in peripheral blood mononuclear cells
    • Petersen N.E., Larsen L.K., Nissen H., et al. Improved RNase protection assay for quantifying LDL receptor mRNA; estimation of analytical imprecision and biological variance in peripheral blood mononuclear cells. Clin. Chem. 41 (1995) 1605-1613
    • (1995) Clin. Chem. , vol.41 , pp. 1605-1613
    • Petersen, N.E.1    Larsen, L.K.2    Nissen, H.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.