Development of a quantitative cell-based ELISA, for a humanized anti-IL-2/IL-15 receptor β antibody (HuMikβ1), and correlation with functional activity using an antigen-transfected murine cell line

Journal of Immunological Methods

Volumn 311, Issue 1-2, 2006, Pages 71-80

  Yang, Xiao Yi ^a   Chen, Eying ^a   Jiang, Hengguang ^a   Hartmann, Wanda K ^a   Mitra, Gautam ^a   Hecht, Toby ^b   Soman, Gopalan ^a  

^a SAIC FREDERICK INC   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Antibody; Assay validation; CbELISA; HuMik 1; IL 15; IL 2 IL 15R ; Proliferation

Indexed keywords

BIOLOGICAL MARKER; CELL ANTIGEN; CHIMERIC ANTIBODY; CHIMERIC PROTEIN; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G1 ANTIBODY; INTERLEUKIN 15; INTERLEUKIN 2 RECEPTOR BETA; INTERLEUKIN 2 RECEPTOR BETA ANTIBODY; INTERLEUKIN 2 RECEPTOR BETA ANTIGEN; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; RECOMBINANT INTERLEUKIN 2 RECEPTOR BETA ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; ASSAY; BINDING AFFINITY; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL LINE; CELL PROLIFERATION; CELL SURFACE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC TRANSFECTION; MOUSE; NONHUMAN; PH; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; STRESS; TITRIMETRY;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL PROLIFERATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; INTERLEUKIN-12; JURKAT CELLS; MICE; RECEPTORS, INTERLEUKIN-15; RECEPTORS, INTERLEUKIN-2; TRANSFECTION;

ANIMALIA; MURINAE;

EID: 33646092304     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.01.014     Document Type: Article

References (25)

1. Abe M., Harpel J.G., Metz C.N., Nunes I., Loskutoff D.J., and Rifkin D.B. An assay for transforming growth factor-β using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. Anal. Biochem. 216 (1994) 276
2. Azimi N., Jacobson S., Leist T., and Waldmann T.A. Involvement of IL-15 in the pathogenesis of human T lymphotropic virus type I-associated myelopathy/tropical spastic paraparesis: implications for therapy with a monoclonal antibody directed to the IL-2/15R β receptor. J. Immunol. 163 (1999) 4064
3. Bamford R.N., Grant A.J., Burton J.D., Peters C., Kurys G., Goldman C.K., Brennan J., Roessler E., and Waldmann T.A. The interleukin (IL) 2 receptor β chain is shared by IL-2 and a cytokine, provisionally designated IL-T, that stimulates T-cell proliferation and the induction of lymphokine-activated killer cells. Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 4940
4. Guex-Crosier Y., Raber J., Chan C.C., Kriete M.S., Benichou J., Pilson R.S., Kerwin J.A., Waldmann T.A., Hakimi J., and Roberge F.G. Humanized antibodies against the α-chain of the IL-2 receptor and against the β-chain shared by the IL-2 and IL-15 receptors in a monkey uveitis model of autoimmune diseases. J. Immunol. 158 (1997) 452
5. Hakimi J., Ha V.C., Lin P., Campbell E., Gately M.K., Tsudo M., Payne P.W., Waldmann T.A., Grant A.J., Tsien W.H., and Schneider W.P. Humanized Mikβ1, a humanized antibody to the IL-2 receptor β-chain that acts synergistically with humanized anti-TAC. J. Immunol. 151 (1993) 1075
6. Hatakeyama M., Tsudo M., Minamoto S., Kono T., Doi T., Miyata T., Miyasaka M., and Taniguchi T. Interleukin-2 receptor β chain gene: generation of three receptor forms by cloned human α and β chain cDNA's. Science 244 (1989) 551
7. Kreitman R.J., Schneider W.P., Queen C., Tsudo M., Fitzgerald D.J., Waldmann T.A., and Pastan I. Mik-beta 1(Fv)-PE40, a recombinant immunotoxin cytotoxic toward cells bearing the beta-chain of the IL-2 receptor. J. Immunol. 149 (1992) 2810
8. Moon K.Y., Hahn B.S., Lee J., and Kim Y.S. A cell-based assay system for monitoring NF-κB activity in human HaCat transfectant cells. Anal. Biochem. 292 (2001) 17
9. Morris J.C., and Waldmann T.A. Advances in IL-2 receptor targeted treatment. Ann. Rheum. Dis. 59 (2000) i109
10. Otani H., Siegel J.P., Erdos M., Gnarra J.R., Toledano M.B., Sharon M., Mostowski H., Feinberg M.B., Pierce J.H., and Leonard W.J. Interleukin (IL)-2 and IL-3 induce distinct but overlapping responses in murine IL-3-dependent 32D cells transduced with human IL-2 receptor beta chain: involvement of tyrosine kinase(s) other than p56lck. Proc. Natl. Acad. Sci. U. S. A. 89 (1992) 2789
11. Y monoclonal antibody B3. Clin. Cancer Res. 6 (2000) 1720
12. Tinubu S.A., Hakimi J., Kondas J.A., Bailon P., Familletti P.C., Spence C., Crittenden M.D., Parenteau G.L., Dirbas F.M., and Tsudo M. Humanized antibody directed to the IL-2 receptor β-chain prolongs primate cardiac allograft survival. J. Immunol. 153 (1994) 4330
13. Tsudo M., Kitamura F., and Miyasaka M. Characterization of the interleukin 2 receptor β chain using three distinct monoclonal antibodies. Proc. Natl. Acad. Sci. U. S. A. 86 (1989) 1982
14. Uttenreuther-Fischer M.M., Huang C.S., and Yu A.L. Pharmacokinetics of human-mouse chimeric anti-GD2 mAb ch14.18 in a phase I trial in neuroblastoma patients. Cancer Immunol. Immunother. 41 (1995) 331
15. Vincenti F., Kirkman R., Light S., Bumgardner G., Pescovitz M., Halloran P., Neylan J., Wilkinson A., Ekberg H., Gaston R., Backman L., and Burdick J. Interleukin-2-receptor blockade with daclizumab to prevent acute rejection in renal transplantation. N. Engl. J. Med. 338 (1998) 161
16. Waldmann T.A. Multichain interleukin-2 receptor: a target for immunotherapy in lymphoma. J. Natl. Cancer Inst. 81 (1989) 914
17. Waldmann T.A. Immune receptors: targets for therapy of leukemia/lymphoma, autoimmune diseases and for the prevention of allograft rejection. Annu. Rev. Immunol. 10 (1992) 675
18. Waldmann T.A. The IL-2/IL-2 receptor system: a target for rational immune intervention. Immunol. Today 14 (1993) 264
19. Waldmann T.A. Immunotherapy: past, present and future. Nat. Med. 9 (2003) 269
20. Waldmann T.A. Targeting the interleukin-15/interleukin-15 receptor system in inflammatory autoimmune diseases. Arthritis Res. Ther. 6 (2004) 174
21. Waldmann T.A., and O'Shea J. The use of antibodies against the IL-2 receptor in transplantation. Curr. Opin. Immunol. 10 (1998) 507
22. Waldmann T.A., and Tagaya Y. The multifaceted regulation of interleukin-15 expression and the role of this cytokine in NK cell differentiation and host response to intracellular pathogens. Annu. Rev. Immunol. 17 (1999) 19
23. Waldmann T.A., White J.D., Goldman C.K., Top L., Grant A., Bamford R., Roessler E., Horak I.D., Zaknoen S., and Kasten-Sportes C. The interleukin-2 receptor: a target for monoclonal antibody treatment of human T-cell lymphotrophic virus I-induced adult T-cell leukemia. Blood 82 (1993) 1701
24. Waldmann T.A., Levy R., and Coller B.S. Emerging therapies: spectrum of applications of monoclonal antibody therapy. Hematology 2000 (2000) 394
25. Yang X.Y., Jiang H., Hartmann W.K., Mitra G., and Soman G. Development of a quantitative antigen-specific cell-based ELISA for the 7G7/B6 monoclonal antibody directed toward IL-2Rα. J. Immunol. Methods 277 (2003) 87