Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples

Journal of Microbiological Methods

Volumn 65, Issue 2, 2006, Pages 350-353

  Moroney, Shannon M ^a   Heller, Loree C ^a   Widen, Ray H ^a  

^a TAMPA GENERAL HOSPITAL   (United States)

Author keywords

gag; HIV 1 proviral DNA; LTR; TaqMan PCR assays

Indexed keywords

TAQ POLYMERASE;

ARTICLE; BLOOD SAMPLING; EVALUATION; GENOME; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY ANALYSIS; VIRUS DETECTION;

DNA, VIRAL; GENES, GAG; HIV INFECTIONS; HIV LONG TERMINAL REPEAT; HIV-1; HUMANS; POLYMERASE CHAIN REACTION; PROVIRUSES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 33646016613     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2005.08.003     Document Type: Article

References (15)

1. Barlow K.L., Tosswill J.H.C., Parry J.V., and Clewley J.P. Performance of the Amplicor human immunodeficiency virus type 1 PCR and analysis of specimens with false-negative results. J. Clin. Microbiol. 35 (1997) 2846-2853
2. Barlow K.L., Tosswill J.H.C., and Clewley J.P. Analysis and genotyping of PCR products of the Amplicor HIV-1 kit. J. Virol. Methods 52 (1995) 65-74
3. Cassol S., Butcher A., Kinard S., Spadaro J., Sy T., Lapointe N., Read S., Gomez P., Fauvel M., Major C., and O'Shaughnessy M. Rapid screening for early detection of mother-to-child transmission of human immunodeficiency virus type 1. J. Clin. Microbiol. 32 (1994) 2641-2645
4. Christopherson C., Kidane Y., Conway B., Krowka J., Sheppard H., and Kwok S. PCR-based assay to quantify human immunodeficiency virus type 1 DNA in peripheral blood mononuclear cells. J. Clin. Microbiol. 38 (2000) 630-634
5. Chun T.W., Carruth L., Finzi D., Shen X., Giuseppe J.A., Taylor H., Hermankova M., Chadwick K., Margolick J., Quinn T.C., Kuo Y.H., Brookmeyer R., Zeiger M.A., Barditch-Crovo P., and Siciliano R.F. Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature 387 (1997) 183
6. Désiré N., Dehée A., Schneider V., Jacomet C., Goujon C., Girard P.M., Rozenbaum W., and Nicolas J.C. Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay. J. Clin. Microbiol. 39 (2001) 1303-1310
7. Dickover R.E., Donovan R.M., Godstein E., Cohen S.H., Bolton V., Huth R.G., Liu G.Z., and Carlson J.R. Decreases in unintegrated HIV DNA are associated with antiretroviral therapy in AIDS patients. J. Acquir. Immune Defic. Syndr. 5 (1992) 31-36
8. Dunn D.T., Brandt C.D., Krivine A., Cassol S.A., Roues P., Borkowsky W., De Rossi A., Denamus E., Ehrnst A., Loveday C., Harris J.-A., McIntosh K., Comeau A.M., Rakusan T., Newell M.-L., and Peckham C.S. The sensitivity of HIV-1 DNA polymerase chain reaction in the neonatal period and the relative contribution of intra-uterine and intra-partum transmission. AIDS 9 (1995) F7-F11
9. Khadir A., Coutlee' F., Saint-Antoine P., Olivier C., Voyer H., and Kesous-Elbaz A. Clinical evaluation of Amplicor HIV-1 test for detection of human immunodeficiency virus type-1 proviral DNA in peripheral blood mononuclear cells. J. Acquired Immune Defic. Syndr. Hum. Retovirol. 9 (1995) 257-263
10. Loussert, Ajaka L., Descamps D., Simon F., Brun-Vezinet F., Ekwalanga M., and Saragosti S. Genetic diversity and HIV detection by polymerase chain reaction. Lancet 346 (1995) 912-913
11. Perelson A.S., Neumann A.U., Markowittz M., Leonard J.M., and Ho D.D. HIV-1 dynamics in vivo: virion clearance rate, infected cell life-span, and viral generation time. Science 271 (1996) 1582-1586
12. Saha B.K., Tian B., and Bucy R.P. Quantitation of HIV-1 by real-time PCR with a unique fluorogenic probe. J. Virol. Methods. 93 (2001) 33-42
13. Tamalet C., Lafeuillade A., Fantini J., Poggi C., and Yahi N. Quantification of HIV-1 viral load in lymphoid and blood cells: assessment during four-drug combination therapy. AIDS 11 (1997) 895-901
14. Yun Z., Fredriksson E., and Sonnerborg A. Quantification of human immunodeficiency virus type 1 proviral DNA by the TaqMan real-time PCR assay. J. Clin. Microbiol. 40 (2002) 3883-3884
15. Zhao Y., Yu M., Miller J.W., Chen M., Bremer E.G., Kabat W., and Yogev R. Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology. J. Clin. Microbiol. 40 (2002) 675-678