Effects of excess corticosterone on NADPH generating enzymes and glucose oxidation in Leydig cells of adult rats

Human and Experimental Toxicology

Volumn 25, Issue 3, 2006, Pages 119-125

  Subramanian Kavitha, T ^a   Parthasarathy, C ^a   Sivakumar, R ^a   Badrinarayanan, R ^a   Balasubramanian, K ^a  

^a UNIVERSITY OF MADRAS   (India)

Author keywords

14C glucose oxidation; Corticosterone; Leydig cell; LH; NADPH generation

Indexed keywords

CORTICOSTERONE 21 ACETATE; ENZYME; GLUCOSE; ISOCITRATE DEHYDROGENASE (NADP); LUTEINIZING HORMONE; PHOSPHOGLUCONATE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TESTOSTERONE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVITY; ENZYME INHIBITION; GLUCOSE OXIDATION; LEYDIG CELL; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; TESTOSTERONE BLOOD LEVEL;

ANIMALS; CORTICOSTERONE; GLUCOSE; ISOCITRATE DEHYDROGENASE; LEYDIG CELLS; MALATE DEHYDROGENASE; MALE; NADP; OXIDATION-REDUCTION; PHOSPHOGLUCONATE DEHYDROGENASE; RATS; RATS, WISTAR; TESTOSTERONE;

ANIMALIA; RATTUS NORVEGICUS;

EID: 33646007705     PISSN: 09603271     EISSN: None     Source Type: Journal    
DOI: 10.1191/0960327106ht591oa     Document Type: Article

References (46)

1. Ansell BM, Bywaters EG. Alternate-day corticosteroid therapy in juvenile chronic polythritis. J Rheumatol 1974; 1: 176-86.
2. Avgerinos PC, Cutler GB, Tsokos GC. Dissociation between cortisol and adrenal androgen secretion in patients receiving alternate-day prednisone therapy. J Clin Endocrinol Metab 1987; 65: 24-29.
3. Veldhuis JD, Lizarralde G, Iranmanesh A. Divergent effects of short term glucocorticoid excess on the gonadotropic and somatotropic axes in normal men. J Clin Endocrinol Metab 1992; 74: 96-102.
4. Gabrilove JL, Nicolis GL, Sohval AR. The testis in Cushing's syndrome. J Urol 1974; 112: 95-99.
5. Horiba N. Sexual and gonadal dysfunction in adrenal disorders. Nippon Rinsho 1997; 55: 2979-84.
6. Balasubramanian K, Pereira BMJ, Govindarajulu P. Epididymal carbohydrate metabolism in experimental hypercorticosteronism - studies on mature male rats. Int J Androl 1982; 5: 534-44.
7. Ravi Sankar B, Maran RRM, Sudha S, Govindarajulu P, Balasubramanian K. Chronic corticosterone treatment impairs Leydig cell 11β-hydroxysteroid dehydrogenase activity and LH-stimulated testosterone production. Horm Metab Res 2000; 32: 142-46.
8. Agular BM, Vind C. Effects of dexamethasone on steroidogenesis in Leydig cells from rats of different ages. J Steroid Biochem Mol Biol 1995; 54: 75-81.
9. Rosen H, Jameel ML, Barkan AL. Dexamethasone suppresses gonadotropin releasing hormone (GnRH) secretion and has direct pituitary effects in male rats: differential regulation of GnRH receptor and gonadotropin responses to GnRH. Endocrinology 1988; 122: 2873-80.
10. Ringstrom SJ, Mc Andrews JM, Rahal JO, Schwartz NB. Cortisol in vivo increases FSH-β mRNA selectively in pituitaries of male rats. Endocrinology 1991; 129: 2793-95.
11. Stalker A, Hermo L, Antakly T. Covalent affinity labelling, radioautography and immunocytochemistry localize the glucocorticoid receptor in rat testicular Leydig cells. Am J Anat 1989; 186: 369-77.
12. Monder C, Miroff Y, Marandici A, Hardy MP. 11β-hydroxysteroid dehydrogenase alleviates glucocorticoid-mediated inhibition of steroidogenesis in rat Leydig cells. Endocrinology 1994; 13: 1199-204.
13. Allan M, Aniko N. Glucocorticoid action: physiology. W.B. Saunders Co., 2001.
14. Mc Mahon M, Gerich J, Rizza R. Effects of glucocorticoids on carbohydrate metabolism. Diab/Metab Rev 1988; 4: 17-30.
15. Shamoon H, Somon V, Sherwin RS. The influence of acute physiological increments of cortisol on fuel metabolism and insulin binding to monocytes in normal humans. J Clin Endocrinol Metab 1980; 50: 495-501.
16. Morita Y, Munck A. Effect of glucocorticoids in vivo and in vitro on net glucose uptake and amino acid incorporation by rat thymus cells. Biochim Biophys Acta 1964; 93: 150-57.
17. Hodgson Y, Hudson B. Leydig cell function. Monogr Endocrinol 1983; 25: 107-32.
18. Blankenstein MA, Van Woetkorn-Blik A, Janszen FHA, Vander Molen HJ. Effects of cofactors and luteinizing hormone on testosterone production by rat testis preparations. J Steroid Biochem 1976; 7: 445-50.
19. Richard JA, Walter LM. The principle, pathway and enzymes of human steroidogenesis. W.B. Saunders Co, 2001.
20. Aldred LF, Cooke BA. The effect of cell damage on the density and steroidogenic capacity of rat testis Leydig cells, using an NADH exclusive test for determination of viability. J Steroid Biochem 1983; 18: 411-14.
21. King J. 6-Phosphogluconate dehydrogenase. Academic Press, 1974.
22. Bernt E, Bergmeyer HU. Isocitrate dehydrogenase. Academic Press, 1974.
23. Ochoa S. Malic enzyme in pigeon liver and wheat germ. Academic Press, 1955.
24. Lowry OH, Rosenbrough NJ, Lewis Fair A, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75.
25. 14C metabolism by mouse, rat and rabbit tissues. Comp Biochem Physiol 1971; 39A: 599-604.
26. Kraft LA, Johnson AD. Epididymal carbohydrate metabolism II. Substrates and pathway utilization of caput and cauda epididymal tissues from the rabbit, rat and mouse. Comp Biochem Physiol 1972; 42B: 451-61.
27. Maric D, Kostic T, Kovacevic R. Effects of acute and chronic immobilization stress on rat Leydig cell steroidogenesis. J Steroid Biochem Mol Biol 1996; 58: 351-55.
28. Lopez-Calderon A, Ariznararreta C, Gonzalez-Quijano MI, Tresgurres JAF, Calderon MD. Stress-induced changes in testis function. J Steroid Biochem Mol Biol 1991; 40: 473-79.
29. Vierhapper H, Nowotay P, Waldhause W. Production rates of testosterone in patients with Cushing's syndrome. Metabolism 2000; 49: 229-31.
30. Ravi Sankar B, Maran RRM, Sivakumar R, Govindarajulu P, Balasubramanian K. Chronic administration of corticosterone impairs LH signal transduction and steroidogenesis in rat Leydig cells. J Steroid Biochem Mol Biol 2000; 72: 155-62.
31. Hardy MP, Gao HB, Dong Q, Ge R, Wang Q, Chai WR et al. Stress hormone and male reproductive function. Cell Tissue Res 2005; 5: 1-7.
32. Ge RS, Dong Q, Niu EM, Sottas CM, Hardy DO, Catterall JF et al. 11(beta)-Hydroxysteroid dehydrogenase 2 in rat Leydig cells: its role in blunting glucocorticoid action at physiological levels of substrate. Endocrinology 2005; 146: 2657-64.
33. Hall PH. Testicular steroid biosynthesis: organization and regulation. Raven Press, 1994.
34. Ravi Sankar B, Maran RRM, Sudha S, Valli G, Govindarajulu P, Balasubramanian K. Corticosterone excess impairs LH signal transduction and steroidogenesis in Leydig cells of adult rat. FASEB J 1999; 13: 624.1.
35. Abraham S, Klein HP, Lin CY, Volkmann C. The effect of space flight on some rat liver enzymes regulating carbohydrate and lipid metabolism. Adv Space Res 1981; 1: 199-217.
36. Valivullah HM, Aruldhas MM, Govindarajulu P. Testicular lipogenic enzymes - direct effects of dexamethasone in vivo. Biochem Biophys Res Commun 1985; 131: 726-30.
37. Revital R, David S, Israel H. Electron leakage from the adrenal cortex mitochondria P450scc and P450c11 systems: NADPH and steroid dependence. Arch Biochem Biophys 1995; 317: 412-16.
38. Iuchi T, Akaike M, Miteui T, Ohshima Y, Shintani Y, Azuma H et al. Glucocorticoid excess induces superoxide production in vascular endothelial cells and elicits vascular endothelial dysfunction. Circ Res 2003; 92: 81-87.
39. Lee SM, Huh TL, Park JW. Inactivation of NADP(+)-dependent isocitrate dehydrogenase by reactive oxygen species. Biochimie 2001; 83: 1057-65.
40. Goad LJ. Cholesterol biosynthesis and metabolism. Blackwell Scientific Publication, 1975.
41. Hanukoglu I, Rapoport R. Routes and regulation of NADPH production in steroidogenic mitochondria. Endocr Res 1995; 21: 231-41.
42. Dimitriadis G, Leighton B, Parry-Billings M, Sasson S, Young M, Krause U et al. Effects of glucocorticoid excess on the sensitivity of glucose transport and metabolism to insulin in rat skeletal muscle. Biochem J 1997; 321: 707-12.
43. Malchoff DM, Maloff BL, James N, Livingston, Lockwood DH. Influence of dexamethasone on insulin action: inhibition of basal and insulin-stimulated hexose transport is dependent on length of exposure in vitro. Endocrinology 1982; 110: 2081-87.
44. Khanum A, Buczko E, Dufau ML. Essential role of adenosine triphosphate in activation of 17β-hydroxysteroid dehydrogenase in the rat Leydig cell. Endocrinology 1997; 138: 1612-20.
45. McKeever BM, Hawkins BK, Geissler WM, Wu L, Sheridan RP, Mosley RT et al. Amino acid substitution of arginine 80 in 17β-hydroxysteroid dehydrogenase type 3 and its effect on NADPH cofactor binding and oxidation/reduction kinetics. Biochim Biophys Acta 2002; 1601: 29-37.
46. Amrolia P, Sullivan MH, Garside D, Baldwin SA, Cooke BA. An investigation of glucose uptake in relation to steroidogenesis in rat testis and tumour Leydig cells. Biochem J 1988; 249: 925-28.