Amino acid substitution of arginine 80 in 17β-hydroxysteroid dehydrogenase type 3 and its effect on NADPH cofactor binding and oxidation/reduction kinetics

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1601, Issue 1, 2002, Pages 29-37

  McKeever, Brian M ^a   Hawkins, Barton K ^b   Geissler, Wayne M ^a   Wu, Ling ^a   Sheridan, Robert P ^a   Mosley, Ralph T ^a   Andersson, Stefan ^b  

^a MERCK RESEARCH LABORATORIES   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

17 Hydroxysteroid dehydrogenase type 3; Amino acid replacement; NADPH binding; Short chain dehydrogenase reductase; Site directed mutagenesis; Transient transfection

Indexed keywords

2' PHOSPHATE; ADENOSINE; ARGININE; ASPARTIC ACID; GLUTAMINE; GLUTATHIONE REDUCTASE; GUANIDINE; ISOLEUCINE; LEUCINE; LYSINE; METHIONINE; PHOSPHATE; PURINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SERINE; TESTOSTERONE 17BETA DEHYDROGENASE; TESTOSTERONE 17BETA DEHYDROGENASE 1; TESTOSTERONE 17BETA DEHYDROGENASE 3; TYROSINE; UNCLASSIFIED DRUG; HYDROXYSTEROID DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; EMBRYO; ENZYME ANALYSIS; HUMAN; HUMAN CELL; HYDROGEN BOND; HYDROPHOBICITY; MOLECULAR INTERACTION; MUTANT; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; X RAY ANALYSIS; CHEMISTRY; GENETICS; KINETICS; MALE; METABOLISM; MOLECULAR GENETICS; PROTEIN BINDING; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

17-HYDROXYSTEROID DEHYDROGENASES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARGININE; CRYSTALLOGRAPHY, X-RAY; HUMANS; KINETICS; MALE; MOLECULAR SEQUENCE DATA; NADP; OXIDATION-REDUCTION; PROTEIN BINDING; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 1242273808     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-9639(02)00434-X     Document Type: Article

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