Molecular cloning of cDNA, recombinant protein expression and characterization of a buckwheat 16-kDa major allergen

International Archives of Allergy and Immunology

Volumn 140, Issue 1, 2006, Pages 73-81

  Koyano, Satoru ^a   Takagi, Kayoko ^a   Teshima, Reiko ^a   Sawada, Jun Ichi ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Allergen; Buckwheat; BWp16; RACE; Recombinant protein; Signal peptide

Indexed keywords

ALBUMIN; ALLERGEN; COMPLEMENTARY DNA; IMMUNOGLOBULIN E; PEPSIN A; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BUCKWHEAT; CALCULATION; CONTROLLED STUDY; DIGESTION; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EVOLUTIONARY HOMOLOGY; GENE AMPLIFICATION; GENETIC CODE; HUMAN; MOLECULAR CLONING; MOLECULAR WEIGHT; NORMAL HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN PURIFICATION; RICINUS COMMUNIS; SEQUENCE HOMOLOGY; SERUM; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ALLERGENS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ENZYME-LINKED IMMUNOSORBENT ASSAY; FAGOPYRUM; HUMANS; HYPERSENSITIVITY; IMMUNOGLOBULIN E; MICE; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EID: 33645995415     PISSN: 10182438     EISSN: None     Source Type: Journal    
DOI: 10.1159/000092038     Document Type: Article

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