Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method

Plant Biotechnology

Volumn 23, Issue 2, 2006, Pages 191-194

  Sjahril, Rinaldi ^a,b   Dong, Poh Chin ^a   Raham, Sher Khan ^a   Yamamura, Saburo ^c   Nakamura, Ikuo ^a   Amemiya, Yoshimiki ^a   Mii, Masahiro ^a  

^a CHIBA UNIVERSITY   (Japan)

^b HASANUDDIN UNIVERSITY   (Indonesia)

^c IWATE BIOTECHNOLOGY RESEARCH CENTER   (Japan)

Author keywords

Agrobacterium tumefaciens; Erwinia carotovora; Phalaenopsis; Wasabi defensin

Indexed keywords

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; EUTREMA WASABI; ORCHIDACEAE; PECTOBACTERIUM CAROTOVORUM; PHALAENOPSIS; RHIZOBIUM;

EID: 33645881095     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.23.191     Document Type: Article

References (21)

1. Anzai H, Ishii Y, Shichinohe M, Katsumata K, Nojiri C, Morikawa H, Tanaka M (1996) Transformation of Phalaenopsis by particle bombardment. Plant Tissue Culture Lett 13: 265-272
2. Belarmino MM, Mii M (2000) Agrobacterium-mediated genetic transformation of a Phalaenopsis orchid. Plant Cell Rep 19: 435-442
3. Bohlmann H (1994) The role of thionins in plant protection. Crit Rev Plant Sci 13: 1-16
4. Broekaert WF, Terras FRG, Cammue BPA, Osborn RW (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol 108: 1353-1358
5. Chakrabarti A, Ganapathi TR, Mukherjee PK, Bapat VA (2003) MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana. Planta 216: 587-596
6. Chia TF, Chan YS, Chua NH (1994) The firefly luciferase gene as a non-invasive reporter for Dendrobium transformation. Plant J 6: 441-446
7. Epple P, Apel K, Bohlmann H (1997) Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell 9: 509-520
8. Fagard M, Vaucheret H (2000) Systemic silencing signal(s). Plant Mol Biol 43: 285-293
9. Florack DEA, Stiekema WJ (1994) Thionins: properties, possible biological roles, and mechanisms of action. Plant Mol Biol 26: 25-37
10. Gao AG, Hakimi SM, Mittanck CA, Wu Y, Woerner BM, Stark DM, Shah DM, Liang J, Rommens CMT (2000) Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat Biotechnol 18: 1307-1310
11. Kanzaki H, Nirasawa S, Saitoh H, Ito M, Nishihara M, Terauchi R, Nakamura I (2002) Overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus (Magnaporthe grisea) in transgenic rice. Theor Appl Genet 105: 809-814
12. Kiba A, Saitoh H, Nishihara M, Omiya K, Yamamura S (2003) C-terminal domain of a hevein-like protein from Wasabia japonica has potent antimicrobial activity. Plant Cell Physiol 44: 296-303
13. th International Workshop on Pathogenesis-related Proteins in Plants; Signalling Pathways and Biological Activities 1998. Aussois, France
14. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 4321-4325
15. Ono H, Tesaki S, Tanabe S, Watanabe M (1998) 6-methylsulfinylhexyl isothiocyanate and its homologues as foodoriginated compounds with antibacterial activity against Escherichia coli and Staphylococcus aureus. Biosci Biotechnol Biochem 62: 363-365
16. Pedras MSC, Sorensen JL, Okanga FI, Zaharia I (1999) Wasalexins A and B, new phytoalexins from wasabi: isolations and synthesis and antifungal activity. Bioorgan Medic Chem Lett 9: 3015-3020
17. Saitoh H, Kiba A, Nishihara M, Yamamura S, Suzuki K, Terauchi R (2001) Production of antimicrobial defensin in Nicotiana benthamiana with a potato virus X vector. Mol Plant Microbe Interact 14: 111-115
18. Sjahril R, Mii M (in press) High-efficiency Agrobacterium-mediated transformation of Phalaenopsis using meropenem, a novel antibiotic to eliminate Agrobacterium. J Hort Sci Biotech
19. Terras FRG, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, Van Leuven F, Vanderleyden J, Cammue BPA, Broekaert WF (1995) Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573-588
20. Thevissen K, Ghazi A, De Samblanx GW, Brownlee C, Osborn RW, Broekaert WF (1996) Fungal membrane responses induced by plant defensins and thionins. J Biol Chem 271: 15018-15025
21. Tokuhara K, Mii M (1993) Micropropagation of Phalaenopsis and Doratenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep 13: 7-11