Atomic force microscopy of native human metaphase chromosomes in a liquid

Archives of Histology and Cytology

Volumn 69, Issue 1, 2006, Pages 73-78

  Hoshi, Osamu ^a   Shigeno, Masatsugu ^b   Ushiki, Tatsuo ^a  

^a NIIGATA UNIVERSITY GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES   (Japan)

^b SII NANOTECHNOLOGY INC   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; HEXYLENE GLYCOL; SILANE;

ADSORPTION; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL STRAIN K 562; CHROMATID; CHROMATIN; CHROMOSOME ISOLATION; CONTROLLED STUDY; FIBER; HUMAN; HUMAN CELL; LIQUID; LYMPHOBLAST; METAPHASE CHROMOSOME;

CELL LINE, TUMOR; CHROMOSOMES, HUMAN; GLYCOLS; HUMANS; METAPHASE; MICROSCOPY, ATOMIC FORCE;

EID: 33645820025     PISSN: 09149465     EISSN: 13491717     Source Type: Journal    
DOI: 10.1679/aohc.69.73     Document Type: Article

References (17)

1. Binnig G, Quate CF, Gerber C: Atomic force microscope. Phys Rev Lett 56: 930-933 (1986).
2. Carrano AV, Gray JW, Langlois RG, Burkhart-Schultz KJ, Van Dilla MA: Measurement and purification of human chromosome by flow cytometry and sorting. Proc Natl Acad Sci 76: 1382-1384 (1979).
3. Fritzsche W, Henderson E: Mapping elasticity of rehydrated metaphase chromosomes by scanning force microscopy. Ultramicroscopy 69:191-200 (1997)
4. Fukushi D, Ushiki T: The structure of C-banded human metaphase chromosomes as observed by atomic force microscopy. Arch Histol Cytol 68: 81-87 (2005).
5. Hoshi O, Ushiki T: Three-dimensional structure of G-banded human metaphase chromosomes observed by atomic force microscopy. Arch Histol Cytol 64: 475-482 (2001).
6. Hoshi O, Owen R, Miles M, Ushiki T: Imaging of human metaphase chromosomes by atomic force microscopy in liquid. Cytogenet Genome Res 107: 28-31 (2004).
7. Kimura E, Hoshi O, Ushiki T: Atomic force microscopy of human metaphase chromosomes after differential staining of sister chromatids. Arch Histol Cytol 67: 171-177 (2004).
8. Laemmli UK, Cheng SM, Adolph KW, Paulson JR, Brown JA, Baumbach WR: Metaphase chromosome structure and the role of non-histone proteins. Cold Spring Harbor Symp Quant Biol 42: 351-360 (1978).
9. Marsden MPF, Laemmli UK: Metaphase chromosome structure: Evidence for a Radial loop model. Cell 17: 849-868 (1979).
10. Rattner JB, Lin CC: Radial loops and helical coils coexist in metaphase chromosomes. Cell 42: 291-196 (1985).
11. Stubblefield E, Wray W: Architecture of the Chinese hamster metaphase chromosome. Chromosome. 32: 262-294 (1971).
12. Tamayo J: Structure of human chromosomes studied by atomic force microscopy. J Struct Biol 141:198-207 (2003).
13. Ushiki T: Atomic force microscopy for imaging living organisms: from DNA to cell motion. In: Micromachines as tools for nanotechnology (Fujita H ed). Springer, Berlin, 2003 (p.121-130)
14. Ushiki T, Hitomi J, Ogura S, Umemoto T, Shigeno M: Atomic force microscopy in histology and cytology. Arch Histol Cytol 59: 421-31 (1996).
15. Ushiki T, Hoshi O, Iwai K, Kimura E, Shigeno M: The structure of human metaphase chromosomes: its histological perspective and new horizons by atomic force microscopy. Arch Histol Cytol 65: 377-90 (2002).
16. Wray W, Stubblefield E: A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH. Exp Cell Res 59: 469-478 (1970).
17. Wray W, Stubblefield E, Humphrey R: Mammalian metaphase chromosomes with high molecular weight DNA isolated at pH 10.5. Nature New Biol 238: 237-238 (1972).