In vitro characterization of human dental pulp cells: Various isolation methods and culturing environments

Cell and Tissue Research

Volumn 324, Issue 2, 2006, Pages 225-236

  Huang, George T J ^a,c   Sonoyama, Wataru ^b   Chen, James ^a,d   Park, Sang Hyuk ^a,e  

^a UCLA SCHOOL OF DENTISTRY   (United States)

^b OKAYAMA UNIVERSITY   (Japan)

^c UNIVERSITY OF MARYLAND DENTAL SCHOOL   (United States)

^d Kyung Hee University ^*  (United States)

^e Kyung Hee University   (South Korea)

Author keywords

Cbfa1; Collagen type I III; Dental pulp cells; Dentin sialophosphoprotein; Human; Reverse transcription polymerase chain reaction; Scanning electron microscopy

Indexed keywords

COLLAGEN GEL; COLLAGEN TYPE 1; COLLAGEN TYPE 3; PHOSPHOPROTEIN; TRANSCRIPTION FACTOR RUNX2;

ADOLESCENT; ADULT; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL LINEAGE; CELL POPULATION; CELL PROLIFERATION; CELL STRUCTURE; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOPLASM; DENTIN; HUMAN; HUMAN CELL; NORMAL HUMAN; ODONTOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING; TISSUE REGENERATION; TOOTH PULP; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SEPARATION; CELLS, CULTURED; COLLAGEN TYPE I; COLLAGEN TYPE II; CORE BINDING FACTOR ALPHA 1 SUBUNIT; DENTAL PULP; DNA PRIMERS; EXTRACELLULAR MATRIX PROTEINS; HUMANS; MICROSCOPY, ELECTRON, SCANNING; ODONTOBLASTS; PHOSPHOPROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIALOGLYCOPROTEINS; SURFACE PROPERTIES; TISSUE ENGINEERING;

EID: 33645818386     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00441-005-0117-9     Document Type: Article

References (45)

1. About I, Bottero MJ, Denato P de, Camps J, Franquin JC, Mitsiadis TA (2000) Human dentin production in vitro. Exp Cell Res 258:33-41
2. Alsberg E, Hill EE, Mooney DJ (2001) Craniofacial tissue engineering. Crit Rev Oral Biol Med 12:64-75
3. Batouli S, Miura M, Brahim J, T.W.Tsutsui, Fisher LW, Gronthos S, Robey PG, Shi S (2003) Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res 82:976-981
4. Bohl KS, Shon J, Rutherford B, Mooney DJ (1998) Role of synthetic extracellular matrix in development of engineered dental pulp. J Biomater Sci Polym Ed 9:749-764
5. Buurma B, Gu K, Rutherford RB (1999) Transplantation of human pulpal and gingival fibroblasts attached to synthetic scaffolds. Eur J Oral Sci 107:282-289
6. Carlson MA, Longaker MT (2004) The fibroblast-populated collagen matrix as a model of wound healing: a review of the evidence. Wound Repair Regen 12:134-147
7. Chan CP, Lan WH, Chang MC, Chen YJ, Lan WC, Chang HH, Jeng JH (2005) Effects of TGF-betas on the growth, collagen synthesis and collagen lattice contraction of human dental pulp fibroblasts in vitro. Arch Oral Biol 50:469-479
8. Chen S, Santos L, Wu Y, Vuong R, Gay I, Schulze J, Chuang HH, Macdougall M (2005) Altered gene expression in human cleidocranial dysplasia dental pulp cells. Arch Oral Biol 50:227-236
9. Couble ML, Farges JC, Bleicher F, Perrat-Mabillon B, Boudeulle M, Magloire H (2000) Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int 66:129-138
10. Di Lenarda R, Cadenaro M, Sbaizero O (2000) Effectiveness of 1 mol L-1 citric acid and 15% EDTA irrigation on smear layer removal. Int Endod J 33:46-52
11. Drury JL, Mooney DJ (2003) Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 24:4337-4351
12. D'Souza R (2002) Development of the pulpodentin complex. In: Hargreaves KM, Goodis HE (eds) Seltzer and Bender's Dental pulp. Quintessence, Carol Stream, pp 13-41
13. Froes JA, Horta HG, Silveira AB da (2000) Smear layer influence on the apical seal of four different obturation techniques. J Endod 26:351-354
14. Grinnell F (2000) Fibroblast-collagen-matrix contraction: growth-factor signalling and mechanical loading. Trends Cell Biol 10:362-365
15. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 97:13625-13630
16. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, DenBesten P, Robey PG, Shi S (2002) Stem cell properties of human dental pulp stem cells. J Dent Res 81:531-535
17. Haas VR, Santos AR, Jr, Wada ML (2001) Behaviour of fibroblastic cells cultured in collagen I using the sandwich technique. Cytobios 106 (Suppl 2):255-267
18. Hosoya S, Matsushima K, Ohbayashi E, Yamazaki M, Shibata Y, Abiko Y (1996) Stimulation of interleukin-1beta-independent interleukin-6 production in human dental pulp cells by lipopolysaccharide. Biochem Mol Med 59:138-143
19. Igarashi M, Irwin CR, Locke M, Mackenzie IC (2003) Construction of large area organotypical cultures of oral mucosa and skin. J Oral Pathol Med 32:422-430
20. Lee KY, Mooney DJ (2001) Hydrogels for tissue engineering. Chem Rev 101:1869-1879
21. Lee KY, Alsberg E, Mooney DJ (2001) Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering. J Biomed Mater Res 56:228-233
22. MacDougall M, Simmons D, Luan X, Nydegger J, Feng J, Gu TT (1997) Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. Dentin phosphoprotein DNA sequence determination. J Biol Chem 272:835-842
23. Mooney DJ, Powell C, Piana J, Rutherford B (1996) Engineering dental pulp-like tissue in vitro. Biotechnol Prog 12:865-868
24. Nakakura-Ohshima K, Watanabe J, Kenmotsu S, Ohshima H (2003) Possible role of immunocompetent cells and the expression of heat shock protein-25 in the process of pulpal regeneration after tooth injury in rat molars. J Electron Microsc (Tokyo) 52:581-591
25. Nakao K, Itoh M, Tomita Y, Tomooka Y, Tsuji T (2004) FGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells. Biochem Biophys Res Commun 325:1052-1059
26. Nakashima M (1991) Establishment of primary cultures of pulp cells from bovine permanent incisors. Arch Oral Biol 36:655-663
27. Nakashima M, Iohara K, Ishikawa M, Ito M, Tomokiyo A, Tanaka T, Akamine A (2004) Stimulation of reparative dentin formation by ex vivo gene therapy using dental pulp stem cells electrotransfected with growth/differentiation factor 11 (Gdf11). Hum Gene Ther 15:1045-1053
28. Ohshima H, Nakakura-Ohshima K, Takeuchi K, Hoshino M, Takano Y, Maeda T (2003) Pulpal regeneration after cavity preparation, with special reference to close spatio-relationships between odontoblasts and immunocompetent cells. Microsc Res Tech 60:483-490
29. Okazaki M, Yoshimura K, Suzuki Y, Harii K (2003) Effects of subepithelial fibroblasts on epithelial differentiation in human skin and oral mucosa: heterotypically recombined organotypic culture model. Plast Reconstr Surg 112:784-792
30. Okiji T (2002) Pulp as a connective tissue. In: Hargreaves KM, Goodis HE (eds) Seltzer and Bender's Dental Pulp. Quintessence, Carol Stream, pp 95-123
31. Onishi T, Kinoshita S, Shintani S, Sobue S, Ooshima T (1999) Stimulation of proliferation and differentiation of dog dental pulp cells in serum-free culture medium by insulin-like growth factor. Arch Oral Biol 44:361-371
32. Park SH, Hsiao GY, Huang GT (2004) Role of substance P and calcitonin gene-related peptide in the regulation of interleukin-8 and monocyte chemotactic protein-1 expression in human dental pulp. Int Endod J 37:185-192
33. Patel T, Park SH, Lin LM, Chiappelli F, Huang GT (2003) Substance P induces interleukin-8 secretion from human dental pulp cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 96:478-485
34. Priam F, Ronco V, Locker M, Bourd K, Bonnefoix M, Duchene T, Bitard J, Wurtz T, Kellermann O, Goldberg M, Poliard A (2005) New cellular models for tracking the odontoblast phenotype. Arch Oral Biol 50:271-277
35. Saito T, Ogawa M, Hata Y, Bessho K (2004) Acceleration effect of human recombinant bone morphogenetic protein-2 on differentiation of human pulp cells into odontoblasts. J Endod 30:205-208
36. Schmalz G, Garhammer P, Schweiki H (1996) A commercially available cell culture device modified for dentin barrier tests. J Endod 22:249-252
37. Schmalz G, Schuster U, Thonemann B, Barth M, Esterbauer S (2001) Dentin barrier test with transfected bovine pulp-derived cells. J Endod 27:96-102
38. Shi S, Gronthos S (2003) Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res 18:696-704
39. Smith AJ (2002) Dentin formation and repair. In: Hargreaves KM, Goodis HE (eds) Seltzer and Bender's Dental Pulp. Quintessence, Carol Stream, pp 41-63
40. Tsukamoto Y, Fukutani S, Shin-Ike T, Kubota T, Sato S, Suzuki Y, Mori M (1992) Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts. Arch Oral Biol 37:1045-1055
41. Vacanti CA, Langer R, Schloo B, Vacanti JP (1991) Synthetic polymers seeded with chondrocytes provide a template for new cartilage formation. Plast Reconstr Surg 88:753-759
42. Vacanti JP (2003) Tissue and organ engineering: can we build intestine and vital organs? J Gastrointest Surg 7:831-835
43. Vernon RB, Sage EH (1996) Contraction of fibrillar type I collagen by endothelial cells: a study in vitro. J Cell Biochem 60:185-197
44. Vidic B, Chen R, Taylor JJ (1972) Functional behavior and structural modification of pulpal fibroblasts cultured in vitro. Archs Arch Anat Histol Embryol 55:51-73
45. Zhu YK, Umino T, Liu XD, Wang HJ, Romberger DJ, Spurzem JR, Rennard SI (2001) Contraction of fibroblast-containing collagen gels: initial collagen concentration regulates the degree of contraction and cell survival. In Vitro Cell Dev Biol Anim 37:10-16