Pyrosequencing protocol requiring a unique biotinylated primer

Clinical Chemistry and Laboratory Medicine

Volumn 44, Issue 4, 2006, Pages 435-441

  Royo, Jose Luis ^a   Pascual, Manuel Hidalgo ^b   Salinas, Ana ^a   Tello, Francisco Jose ^b   Rivero, Maria Del Carmen ^a   Herrero, Eduardo Ferrero ^b   Real, Luis Miguel ^a   Ruiz, Agustín ^a  

^a Neocodex SL   (Spain)

^b HOSPITAL UNIVERSITARIO 12 DE OCTUBRE   (Spain)

Author keywords

Calpain 10 (CAPN10); Caveolin 1 (CAV1); Cytochrome P450 (CYP19A1); Genotyping; M13; Pyrosequencing

Indexed keywords

CALPAIN 10; CAVEOLIN 1; CYTOCHROME P450 19A1; PYROPHOSPHATE; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; BIOTINYLATION; GENE MAPPING; GENOTYPE; PRIORITY JOURNAL; PYROSEQUENCING; SINGLE NUCLEOTIDE POLYMORPHISM; STOICHIOMETRY; TECHNIQUE;

BIOTINYLATION; DNA PRIMERS; GENOMICS; HUMANS; METHODS; MUTATION; POLYMORPHISM, GENETIC; SEQUENCE ANALYSIS, DNA;

EID: 33645778580     PISSN: 14346621     EISSN: 14346621     Source Type: Journal    
DOI: 10.1515/CCLM.2006.072     Document Type: Article

References (16)

1. Hinds DA, Stuve LL, Nilsen GB, Halperin E, Eskin E, Ballinger DG, et al. Whole-genome patterns of common DNA variation in three human populations. Science 2005;307:1072-9.
2. Tyagi S, Bratu DP, Kramer FR. Multicolor molecular beacons for allele discrimination. Nat Biotechnol 1998;16:49-53.
3. Ronaghi M, Uhlen M, Nyren P. A sequencing method based on real-time pyrophosphate. Science 1998;281:363-5.
4. Hardenbol P, Baner J, Jain M, Nilsson M, Namsaraev EA, Karlin-Neumann GA, et al. Multiplexed genotyping with sequence-tagged molecular inversion probes. Nat Biotechnol 2003;21:673-8.
5. O'Meara D, Ahmadian A, Odeberg J, Lundeberg J. SNP typing by a pyrase-mediated allele-specific primer extension on DNA microarrays. Nucleic Acids Res 2002;30:e75.
6. Kruckeberg KE, Thibodeau SN. Pyrosequencing technology as a method for the diagnosis of multiple endocrine neoplasia type 2. Clin Chem 2004;50:522-9.
7. Frey UH, Nuckel H, Dobrev D, Manthey I, Sandalcioglu IE, Eisenhardt A, et al. Quantification of G protein Gαs sub-unit splice variants in different human tissues and cells using pyrosequencing. Gene Expr 2005;12:69-81.
8. Soderback E, Zackrisson AL, Lindblom B, Alderborn A. Determination of CYP2D6 gene copy number by pyrosequencing. Clin Chem 2005;51:522-31.
9. Pielberg G, Day AE, Plastow GS, Andersson L. A sensitive method for detecting variation in copy numbers of duplicated genes. Genome Res 2003;13:2171-7.
10. Sivertsson A, Platz A, Hansson J, Lundeberg J. Pyrosequencing as an alternative to single-strand conformation polymorphism analysis for detection of N-ras mutations in human melanoma metastases. Clin Chem 2002;48:2164-70.
11. Aquilante CL, Lobmeyer MT, Langaee TY, Johnson JA. Comparison of cytochrome P450 2C9 genotyping methods and implications for the clinical laboratory. Pharmacotherapy 2004;24:720-6.
12. Nordfors L, Jansson M, Sandberg G, Lavebratt C, Sengul S, Schalling M, et al. Large-scale genotyping of single nucleotide polymorphisms by Pyrosequencing™ and validation against the 5′-nuclease (Taqman) assay. Hum Mutat 2002;19:395-401.
13. Gharizadeh B, Eriksson J, Nourizad N, Nordstrom T, Nyren P. Improvements in pyrosequencing technology by employing Sequenase polymerase. Anal Biochem 2004;330:272-80.
14. Nordstrom T, Nourizad K, Ronaghi M, Nyren P. Method enabling pyrosequencing on double-stranded DNA. Anal Biochem 2000;282:186-93.
15. Fakhrai-Rad H, Pourmand N, Ronaghi M. Pyrosequencing: an accurate detection platform for single nucleotide polymorphisms. Hum Mutat 2002;19:479-85.
16. Pacey-Miller T, Henry R. Single-nucleotide polymorphism detection in plants using a single-stranded pyrosequencing protocol with a universal biotinylated primer. Anal Biochem 2003;317:166-70.