rhEPO (recombinant human eosinophil peroxidase): Expression in Pichia pastoris and biochemical characterization

Biochemical Journal

Volumn 395, Issue 2, 2006, Pages 295-301

  Ciaccio, Chiara ^a,b   Gambacurta, Alessandra ^a   De Sanctis, Giampiero ^c   Spagnolo, Domenico ^c   Sakarikou, Christina ^c   Petrella, Giovanni ^a   Coletta, Massimo ^a,b  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b InterUniversity Consortium for the Research on the Metal Chemistry in Biological Systems (CIRCMSB)   (Italy)

^c UNIVERSITY OF CAMERINO   (Italy)

Author keywords

5 thio 2 nitrobenzoic acid (TNB); Eosinophil peroxidase (EPO); Glycosylation; Pichia pastoris; Purification; Thiocyanate (SCN )

Indexed keywords

BIOCHEMISTRY; BLOOD; CHROMATOGRAPHIC ANALYSIS; CLONING; DIMERS; PURIFICATION; YEAST;

5-THIO-2-NITROBENZOIC ACID (TNB); EOSINOPHIL PEROXIDASE (EPO); GLYCOSYLATION; PICHIA PASTORIS; THIOCYANATE (SCN-);

ENZYME KINETICS;

PEROXIDASE; RECOMBINANT HUMAN EOSINOPHIL PEROXIDASE; UNCLASSIFIED DRUG; EOSINOPHIL PEROXIDASE; RECOMBINANT PROTEIN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CHEMICAL ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; ANIMAL; BIOASSAY; BIOSYNTHESIS; ENZYME SPECIFICITY; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PICHIA; SPECTROSCOPY; SWINE;

MAMMALIA; PICHIA PASTORIS;

ANIMALS; BIOLOGICAL ASSAY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; EOSINOPHIL PEROXIDASE; GENE EXPRESSION; HUMANS; KINETICS; MOLECULAR WEIGHT; PICHIA; RECOMBINANT PROTEINS; SPECTRUM ANALYSIS; SUBSTRATE SPECIFICITY; SWINE;

EID: 33645755615     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20051385     Document Type: Article

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