Polymerase chain reaction analysis of transgenic plants contaminated by Agrobacterium

Plant Molecular Biology Reporter

Volumn 23, Issue 1, 2005, Pages 59-65

  Nain, Vikrant ^a   Jaiswal, Rajani ^a   Dalal, Monika ^a   Ramesh, Bandarupalli ^b   Kumar, Polumetla A ^a  

^a NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY   (India)

^b CH CHARAN SINGH UNIVERSITY   (India)

Author keywords

Agrobacterium contamination; DNA renaturation; PCR; Transgenic plants

Indexed keywords

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; RHIZOBIUM;

EID: 33645555620     PISSN: 07359640     EISSN: 07359640     Source Type: Journal    
DOI: 10.1007/BF02772647     Document Type: Article

References (13)

1. Birnboim HC (1983) A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol 100: 243-255.
2. Chen PY, Wang CK. Soong SC, and To KY (2003) Complete sequence of the binary vector pBI121 and its application in cloning T-DNA from transgenic plants. Mol Breed 11: 287-293.
3. De Buck S. De Wilde C, Van Montagu M, and Depicker A (2000) T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation. Mol Breed 6: 459-468.
4. Doyle JJ and Doyle JI (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
5. Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the "Gene-Jockeying" tool. Microbiol Mol Biol Rev 67: 16-37.
6. Hofgen R and Willmtzer L (1988) Storage of competent, cells of Agrobacterium transformation. Nucleic Acids Res 16(20): 9877.
7. Horsch RB, Fry JE, Huffman NL, Eichholtz D, Ragers SG, and Fraley RT (1985) A simple method for transferring genes into plants. Science 227: 1229-1231.
8. Nauerby B, Billing K, and Wyndaele R (1997) Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of plant cultures. Plant Cell Tissue Organ Cult 29: 153-160.
9. Sambrook J, Fritsch EF, and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2d ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
10. Sarma KS, Evans NE, and Selby C (1995) Effect of carbenicillin and cefotaxime on somatic embryogenesis of Sitka spruce (Picea sitchensis (Bong.) Carr.). J Exp Bot 46: 1779-1781.
11. Shackelford NJ and Chlan CA (1996) Identification of antibiotics that are effective in eliminating Agrobacterium tumefaciens. Plant Mol Biol Rep 14: 50-57.
12. Siritunga D and Sayre RT (2003) Generation of cyanogen-free transgenic cassava. Planta 217: 367-373.
13. Trontin JF, Harvengt L, Garin E, Lopez-Vernaza MLV, Arancio L, Hoebeke J, Canlet F, and Paques M (2002) Towards genetic engineering of maritime pine (Pinus pinaster Ait.) Ann Forest. Sci 59: 687-697.