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Volumn 93, Issue 26 I, 2004, Pages

Joining and scission in the self-assembly of nanotubes from DNA tiles

Author keywords

[No Author keywords available]

Indexed keywords

DNA NANOTUBES; OLIGONUCLEOTIDES; PROTEIN POLYMERS; SCISSION EVENTS;

EID: 33645471225     PISSN: 00319007     EISSN: 10797114     Source Type: Journal    
DOI: 10.1103/PhysRevLett.93.268301     Document Type: Article
Times cited : (65)

References (28)
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  • 15
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    • note
    • HPLC purified DNA oligomers were purchased from Integrated DNA Technologies (Coralville, IA). Each oligomer was brought to a stock concentration of 10 μM in pure water, as determined by UV absorbance, and stored at 20 °C. The five oligomer stock solutions were combined and diluted to a final concentration of 400 nM each in buffer whose final concentration was 40 mM tris-acetate, 1 mM ethylene diamine tetra-acetic acid, and 12.5 mM Mg-acetate at a pH of 8.3.
  • 16
    • 13444295533 scopus 로고    scopus 로고
    • note
    • The fluorophore, either fluorescein (FAM) or carboxytetramethylrhodamine (TAMRA), was located at the 3′ end of either the 1, 3, or 5 strand [Fig. 1(e)], with no apparent position dependent effect An oxygen scavenging system enables extended observation with minimal photobleaching. Samples were prepared for imaging, by adding 1 μtL of a 10× oxygen scavenging mix (45 mg/ml glucose, 2.0 mg/ml glucose oxidase, 0.35 mg/ml catalase, 5% β- mercaptoethanol) to 9 μL of nanotube solution. Then 1.5 to 2.5 μL of the resulting solution was deposited onto a slide, covered with a cover slip, and sealed with 5-min epoxy.
  • 17
    • 0031185732 scopus 로고    scopus 로고
    • ++ in the buffer solution mediates the strong adsorption of DNA on bare glass. The deposition is not homogeneous across the sample, however. In some regions, immobilized nanotubes appear locally aligned, presumably due to flows generated during sample preparation. In other regions, an excess of short tubes suggests that the resulting shear can be strong enough to break tubes, potentially biasing the distribution toward shorter lengths. PVP coating prevents nanotubes from sticking to the glass, ensuring that measurements are not confounded by breaking or bending induced by the strong surface interactions. The coating procedure was adapted from K. Srinivasan, G. Pohl, and N. Avdalovic, [Anal. Chem. 69, 2798 (1997)]. Before coating, slides and cover slips were cleaned by sonication in soapy water (Micro, Cole Parmer) at 60 °C for 30′, rinsed copiously in deionized water and stored in ethanol.
    • (1997) Anal. Chem. , vol.69 , pp. 2798
    • Srinivasan, K.1    Pohl, G.2    Avdalovic, N.3
  • 20
    • 0023789421 scopus 로고
    • D. B. Murphy et al., J. Cell Biol. 106, 1947 (1988); S. W. Rothwell et al., J. Cell Biol. 102, 619 (1986).
    • (1988) J. Cell Biol. , vol.106 , pp. 1947
    • Murphy, D.B.1
  • 21
    • 0022575638 scopus 로고
    • D. B. Murphy et al., J. Cell Biol. 106, 1947 (1988); S. W. Rothwell et al., J. Cell Biol. 102, 619 (1986).
    • (1986) J. Cell Biol. , vol.102 , pp. 619
    • Rothwell, S.W.1
  • 22
    • 0042121256 scopus 로고    scopus 로고
    • ++. Bases were added at each end to take into account stacking interactions, but were not allowed to pair.
    • (2003) Nucleic Acids Res. , vol.31 , pp. 3406
    • Zuker, M.1
  • 26


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.