Enrichment and analysis of RNA centered on ion pair reverse phase methodology

RNA

Volumn 12, Issue 4, 2006, Pages 691-696

  Dickman, Mark J ^a   Hornby, David P ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Ion pair reverse phase chromatography; Small RNAs and miRNA; Solid phase extraction; Total RNA

Indexed keywords

MICRORNA; RIBOSOME RNA;

ARTICLE; CHEMICAL ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION PAIR CHROMATOGRAPHY; PRIORITY JOURNAL; RNA ANALYSIS; RNA EXTRACTION; SOLID PHASE EXTRACTION;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; RNA;

EID: 33645465806     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.2278606     Document Type: Article

References (20)

1. Ambros, V. 2004. The functions of animal microRNAs. Nature 431: 350-355.
2. Azarani, A. and Hecker, K.H. 2001. RNA analysis by ion-pair reversed-phase high performance liquid chromatography. Nucleic Acids Res. 29: e7. http://nar.oupjournals.org.
3. Bailey, J.M. and Davidson, N. 1976. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal. Biochem. 70: 75-85.
4. Bartel, D.P. 2004. MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116: 281-297.
5. Boom, R., Sol, C.J., Salimans, M.M., Jansen, C.L., Wertheim-van Dillen, P.M., and van der Noordaa, J. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28: 495-503.
6. Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
7. Floyd, R.W., Stone, M.P., and Joklik, W.K. 1974. Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Anal. Biochem. 59: 599-609.
8. Gjerde, D.T., Hanna, C.P., and Hornby, D.P. 2002. DNA chromatography. Wiley-VCH, Weinheim, Germany.
9. Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N., and Altman, S. 1983. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell 35: 849-857.
10. Huber, C.G. 1998. Micropellicular stationary phases for high-performance liquid chromatography of double-stranded DNA. J. Chromatogr. A 806: 3-30.
11. Huber, C.G., Oefner, P.J., Preuss, E., and Bonn, G.K. 1993. High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles. Nucleic Acids Res. 21: 1061-1066.
12. Kruger, K., Grabowski, P.J., Zaug, A.J., Sands, J., Gottschling, D.E., and Cech, T.R. 1982. Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell 31: 147-157.
13. Lehrach, H., Diamond, D., Wozney, J.M., and Boedtker, H. 1977. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical re-examination. Biochemistry 16: 4743-4751.
14. McMaster, G.K. and Carmichael, G.G. 1977. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc. Natl. Acad. Sci. 74: 4835-4838.
15. Oberacher, H. and Huber, C.G. 2002. Capillary monoliths for the analysis of nucleic acids by high performance liquid chromatography-electrospray ionization mass spectrometry. Trends Anal. Chem. 21: 166-174.
16. Pinder, J.C., Staynov, D.Z., and Gratzer, W.B. 1974. Electrophoresis of RNA in formamide. Biochemistry 13: 5373-5378.
17. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: A laboratory manual, 2d ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Sebo, T.J. and Schmit, J.C. 1982. Analytical gel electrophoresis of high-molecular-weight RNA in acrylamide-agarose gels containing methylmercuric hydroxide. Anal. Biochem. 120: 136-145.
19. Tsang, S.S., Yin, X., Guzzo-Arkuran, C., Jones, V.S., and Davison, A.J. 1993. Loss of resolution in gel electrophoresis of RNA: A problem associated with the presence of formaldehyde gradients. Biotechniques 14: 380-381.
20. Walcher, W., Oberacher, H., Troiani, S., Holzl, G., Oefner, P., Zolla, L., and Huber, C.G. 2002. Monolithic capillary columns for liquid chromatography-electrospray ionization mass spectrometry in proteomic and genomic research. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 782: 111-125.