Expression and characterization of human vascular endothelial growth factor (VEGF165) in insect cells

Protein Expression and Purification

Volumn 46, Issue 2, 2006, Pages 503-509

  Lee, Geum Young ^a   Jung, Woon Won ^a   Kang, Chang Soo ^b   Bang, In Seok ^a  

^a My Gene Bioscience Institute   (South Korea)

^b Hoseo University   (South Korea)

Author keywords

Angiogenesis; Baculovirus; Human VEGF165; Spodoptera frugiperda

Indexed keywords

DISULFIDE; RECOMBINANT PROTEIN; VASCULAR ENDOTHELIAL GROWTH FACTOR A, HUMAN; VASCULOTROPIN A;

ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; BIOSYNTHESIS; CELL LINE; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; GENETICS; GLYCOSYLATION; HUMAN; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METHODOLOGY; PROTEIN PROCESSING;

ANIMALS; BACULOVIRIDAE; CELL LINE; CHROMATOGRAPHY, LIQUID; DISULFIDES; GLYCOSYLATION; HUMANS; PROTEIN MODIFICATION, TRANSLATIONAL; RECOMBINANT PROTEINS; SPODOPTERA; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

INSECTA; LEPIDOPTERA; MAMMALIA; SPODOPTERA FRUGIPERDA;

EID: 33645419298     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.09.023     Document Type: Article

References (40)

1. B.A. Keyt, H.V. Nguyen, L.T. Berleau, C.M. Duarte, J. Park, H. Chen, and N. Ferrara Identification of vascular endothelial growth factor determinants for binding KDR and FLT-1 receptors J. Biol. Chem. 271 1996 5638 5646
2. N. Ferrara Molecular and biological properties of vascular endothelial growth factor J. Mol. Med. 77 1999 527 543
3. G. Neufeld, T. Cohen, S. Gengrinovitch, and Z. Poltorak Vascular endothelial growth factor (VEGF) and its receptors FASEB J. 13 1999 9 12
4. N. Ferrara, J. Winer, and T. Burton Aortic smooth muscle cells express and secrete vascular endothelial growth factor Growth Factors 5 1991 141 148
5. E. Tisher, R. Mitchell, T. Hartman, M. silva, D. Gospodarowicz, J.C. Fiddes, and J.A. Abraham The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing J. Biol. Chem. 266 1991 11947 11954
6. Z. Poltorak, T. Cohen, R. Sivan, Y. Kandelis, G. Spira, I. Vlodavsky, E. Keshet, and G. Neufeld VEGF145, a secreted vascular endothelial growth factor isoform that binds to extracellular matrix J. Biol. Chem. 272 1997 7151 7158
7. L. Jingjing, Y. Xue, N. Agarwal, and R.S. Roque Human muller cell express VEGF183, a novel spliced variant of vascular endothelial growth factor Invest. Opthalmol. Vis. Sci. 40 1999 752 759
8. 165 via its exon 7-encoded domain J. Biol. Chem. 271 1996 5761 5767
9. D. Belotti, P. Paganoni, L. Manenti, A. Garofalo, S. Marchini, G. Taraboletti, and R. Giavazzi Matrix metalloproteinases (MMP9 and MMP2) induce the release of vascular endothelial growth factor (VEGF) by ovarian carcinoma cells: implications for ascites formation Cancer Res. 63 2003 5224 5229
10. P. Carmeliet Angiogenesis in health and disease Nat. Med. 9 2003 653 660
11. M. Fernandez, F. Vizzutti, J.C. Garcia-Pagan, J. Rodes, and J. Bosch Anti-VEGF receptor-2 monoclonal antibody prevents portalsystemic collateral vessel formation in portal hypertensive mice Gastroenterology 126 2004 886 894
12. 165 with soluble and cell associated flk-1 receptors J. Biol. Chem. 269 1994 12456 12461
13. H. Gitay-Goren, T. Cohen, S. Tessler, S. Soker, S. Gengrinovich, P. Rockwell, M. Klagsbrun, B.Z. Levi, and G. Neufeld Selective binding of VEGF to one of the three vascular endothelial growth factor receptors of vascular endothelial cells J. Biol. Chem. 271 1996 5519 5523
14. S. Soker, S. Takashima, H.Q. Miao, G. Neufeld, and M. Klagsbrun Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor Cell 92 1998 735 745
15. M. Shibuya Structure and function of VEGF/VEGF-receptor system involved in angiogenesis Cell Struct. Funct. 26 2001 25 35
16. 165 gene therapy Valentis Inc Curr. Opin. Mol. Ther. 3 2001 97 101
17. 165 enhances wound healing Gene Ther. 9 2002 777 785
18. X. Wu, Z. Yin, C. Cao, L. Huang, W. Lu, J. Lin, and W. Cui Expression of human VEGF165 in silkworm (Bombyx mori L.) by using a recombinant baculovirus and its bioactivity assay J. Biotechnol. 111 2004 253 261
19. R.D. Possee Baculoviruses as expression vectors Curr. Opin. Biotechnol. 8 1997 569 572
20. R. Verma, E. Boleti, and A.J.T. George Antibody engineering-comparison of bacterial, yeast, insect and mammalian expression systems J. Immunol. Methods 216 1998 165 181
21. U. Seppala, P. Hagglund, P.A. Wurtzen, H. Ipsen, P. Thorsted, T. Lenhard, P. Roepstorff, and M.D. Spangfort Molecular characterization of major cat allergen Fel d 1: expression of heterodimer by use of Baculovirus expression system J. Biol. Chem. 280 2004 3208 3216
22. A. Groner Specificity and safety of baculoviruses R.R. Granados B.A. Federici The Biology of Baculoviruses 1986 CRC Press Boca Raton, FL, USA 177
23. T.A. Pfeifer Expression of heterologous proteins in stable insect cell culture Curr. Opin. Biotechnol. 9 1998 518 521
24. F. Altmann, E. Staudacher, I.B. Wilson, and L. Marz Insect cells as hosts for the expression of recombinant glycoproteins Glycoconj. J. 16 1999 109 123
25. T.A. Kost, and J.P. Condreay Recombinant baculovirus as mammalian cell gene delivery vectors Trends Biotechnol. 20 2002 173 180
26. P. Roy Baculovirus solves a complex problem Nat. Biotechnol. 22 2004 1527 1528
27. L. Ikonomou, Y.J. Schneider, and S.N. Agathos Insect cell culture for industrial production of recombinant proteins Appl. Microbiol. Biotechnol. 62 2003 1 20
28. D. Bahia, R. Cheung, M. Buchs, S. Geisse, and I. Hunt Optimisation of insect cell growth in deep-well blocks: development of a high-throughput insect cell expression screen Protein Express. Purif. 39 2005 61 70
29. A.A. Davis, P.S. Bernstein, D. Bok, J. Turner, M. Nachtigal, and R.C. Hunt A human retinal pigment epithelial cell line that retains epithelial characteristics after prolonged culture Investig. Ophthalmol. Vis. Sci. 36 1995 955 964
30. M.D. Summers, and G.E. Smith A manual of methods for baculovirus vectors and insect cell culture conditions Tex. Agric. Exp. Stn. Bull. No. 1555 1987
31. U.K. Laemmli Cleavage of structural protein during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
32. H. Towbin, T. Staehelin, and J. Gordon Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc. Natl. Acad. Sci. USA 76 1979 43504
33. D. Peretz, H. Gitay-Goren, M. Safran, N. Kimmel, D. Gospodarowicz, and G. Neufeld Glycosylation of vascular endothelial growth factor is not required for its mitogen activity Biochem. Biophys. Res. Commun. 182 1992 1340 1347
34. B.L. Fiebich, B. Jager, C. Schollmann, K. Weindel, J. Wilting, G. Kochs, D. Marme, H. Hug, and H.A. Weich Synthesis and assembly of functionally active human vascular endothelial growth factor homodimers in insect cells Eur. J. Biochem. 211 1993 19 26
35. C. Greenfield, G. Patel, S. Clark, N. Jones, and M.D. Waterfield Expression of the human EGF receptor with ligand-stimulatable kinase activity in insect cells using a baculovirus vector EMBO J. 7 1988 139 146
36. T.K. Yeo, D.R. Senger, H.F. Dvorak, L. Freter, and K.T. Yeo Glycosylation is essential for efficient secretion but not for permeability enhancing activity of vascular permeability factor (vascular endothelial growth factor) Biochem. Biophys. Res. Commun. 179 1991 1568 1579
37. T. Cohen, H. Gitay-Goren, G. Neufeld, and B.Z. Levi High levels of biologically active vascular endothelial growth factor (VEGF) are produced by the baculovirus expression system Growth Factors 7 1992 131 138
38. D.W. Leung, G. Cachianes, W.J. Kuang, D.V. Goeddel, and N. Ferrara Vascular endothelial growth factor is a secreted angiogenic mitogen Science 246 1998 1306 1309
39. O. Chavand, K. Spilsbury, and P.E. Rakoczy Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity Biochem. Cell Biol. 79 2001 107 112
40. 165 variant by a site-directed mutation at the plasmin sensitive cleavage site FEBS Lett. 531 2002 309 313