Proteinase K digestion of proteins improves detection of bacterial endotoxins by the Limulus amebocyte lysate assay: Application for endotoxin removal from cationic proteins

Analytical Biochemistry

Volumn 259, Issue 1, 1998, Pages 42-47

  Petsch, D ^a   Deckwer, W D ^a   Anspach, F B ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Endotoxin; Endotoxin protein complexes; LAL assay; Proteinase K

Indexed keywords

AMINO ACIDS;

BACTERIAL ENDOTOXINS; CATIONIC PROTEINS; ENDOTOXIN; ENDOTOXIN REMOVAL; LIMULUS AMEBOCYTE LYSATE; PROTEIN COMPLEXES; PROTEINASE K; SELECTIVE ADSORPTION;

PROTEINS;

CHYMOTRYPSIN; ENDOTOXIN; IMMUNOGLOBULIN G; LYSOZYME; POLYETHYLENEIMINE; POLYLYSINE; PRONASE; PROTEINASE K; RIBONUCLEASE A; TRYPSIN;

ADSORPTION; ANIMAL CELL; ARTICLE; CELL LYSATE; COMPLEX FORMATION; ENZYME ASSAY; LIMULUS; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; WASTE MANAGEMENT;

EID: 0032524393     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1998.2655     Document Type: Article

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