Single-step purification by lectin affinity and deglycosylation analysis of recombinant human and porcine deoxyribonucleases I expressed in COS-7 cells

Biotechnology Letters

Volumn 28, Issue 4, 2006, Pages 215-221

  Fujihara, Junko ^a   Hieda, Yoko ^a   Xue, Yuying ^a   Okui, Izumi ^a   Kataoka, Kaori ^a   Takeshita, Haruo ^a  

^a SHIMANE UNIVERSITY   (Japan)

Author keywords

Con A; DNase I; Human; Lectin affinity column; Pig; Wheatgerm agglutinin

Indexed keywords

CON A; DNASE I; LECTIN-AFFINITY COLUMN; WHEATGERM AGGLUTININ;

CARBOHYDRATES; CELL CULTURE; DNA;

ENZYME KINETICS;

LECTIN; RECOMBINANT PROTEIN; TYPE I SITE SPECIFIC DEOXYRIBONUCLEASE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; FRACTIONATION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; SWINE;

ANIMALS; CERCOPITHECUS AETHIOPS; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, AFFINITY; COS CELLS; DEOXYRIBONUCLEASES, TYPE I SITE-SPECIFIC; GLYCOSYLATION; LECTINS; RECOMBINANT PROTEINS; SWINE;

SUIDAE; SUS SCROFA; TRITICUM AESTIVUM;

EID: 33645323518     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-005-5522-3     Document Type: Article

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