Purification and characterization of a deoxyriboendonuclease from mycobacterium smegmatis

Journal of Biochemistry and Molecular Biology

Volumn 39, Issue 2, 2006, Pages 140-144

  Mandal, Prajna ^a   Chakraborty, Phulghuri ^a   Sau, Subrata ^a   Mandal, Nitai Chandra ^a  

^a BOSE INSTITUTE   (India)

Author keywords

Deoxyriboendonuclease; M. smegmatis; Mycobacteria

Indexed keywords

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS;

EID: 33645276799     PISSN: 12258687     EISSN: 12258687     Source Type: Journal    
DOI: 10.5483/bmbrep.2006.39.2.140     Document Type: Article

References (27)

1. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
2. Brnakova, Z., Godany, A. and Timko, J. (2005) An extracellular endodeoxyribonuclease from Streptomyces aureofaciens. Biochlm. Blophys. Acta 1721, 116-123.
3. Baranovskii, A. G., Buneva, V. N. and Nevinsky, G A. (2004) Human deoxyribonucleases. Biochemistry 69, 587-601.
4. Chaudhuri, B., Sau, S., Datta, H. J. and Mandai, N. C. (1993) Isolation, characterization, and mapping of temperaturesensitive mutations in the genes essential for lysogenic and lytic growth of the mycobacteriophage LI. Virology 194, 166-172.
5. Chevalier, B. S. and Stoddard, B. L. (2001) Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res. 29, 3757-3774.
6. Dryden, D. T., Murray, N. E. and Rao, D. N. (2001) Nucleoside triphosphate-dependent restriction enzymes. Nucleic Acids Res. 29, 3728-3741.
7. Ganesh, N. and Muniyappa, K. (2003) Mycobacterhm smegmatis RecA protein is structurally similar to but functionally distinct from Mycobacterium tuberculosis RecA. Proteins 53, 6-17.
8. Griffin, T. J. 4th, Parsons, L., Leschziner, A. E., DeVost, J., Derbyshire, K. M. and Grindley, N. D. (1999) In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis. Nucleic Acids Res. 27, 3859-3865.
9. Guhan, N. and Muniyappa, K. (2002) Mycobacterium tuberculosis RecA intein possesses a novel ATP-dependent site-specific double-stranded DNA endonuclease activity. J. Biol. Chem. 277, 16257-16264.
10. Hatfull, G. F. (2000) Molecular genetics of mycobacteriophages; in Molecular Genetics of Mycobacteria, Hatfull, G F. and Jacob, W. R., Jr. (eds.), pp 37- 54, ASM Press, Washington D. C., USA.
11. Kishi, K., Yasuda, T. and Takeshita, H. (2001) DNase I: structure, function, and use in medicine and forensic science. Leg Med. 3, 69-83.
12. Krogh, B. O. and Symington, L. S. (2004) Recombination proteins in yeast. Annu. Rev. Genet. 38, 233-271.
13. Martinez-Canamero, M. M., Munoz, J., Extremere, A. L. and Arias, J. M. (1991) Deoxyribonuclease activities in Myxococcus coralloides D. J. Appl. Bacterial. 71, 170-175.
14. Miller, E. S., Kutter, E., Mosig, G., Arisaka, F., Kunisawa, T. and Ruger, W. (2003) Bacteriophage T4 genome. Microbiol. Mol. Blol. Rev. 67, 86-156.
15. Murmur, J. (1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3, 20-218.
16. Papavinasasundaram, K. G., Movahedzadeh, F., Keer, J. T., Stoker, N. G., Colston, M. J. and Davis, E. O. (1997). Mycobacterial recA is cotranscribed with a potential regulatory gene called recX. Mol Microbiol. 24, 141-153.
17. Pingoud, A. and Jeltsch, A. (2001) Structure and function of type II restriction endonucleases. Nucleic Acids Res. 29, 3705-3727.
18. Radding, C. M. (1964) Nuclease activity in defective lysogens of phage lambda. Biochem. Biophys. Res. Commun. 15, 8-12.
19. Rama, J. M., Perez Urena, M. T., Lopez, P. and Espinosa, M. (1987) Characterization of intracellular deoxyribonucleases of Bacillus subtilis by SDS-polyacrylamide gel electrophoresis. Microbios. 49, 199-212.
20. rd ed., Cold Spring Harbor Laboratory Press, New York, USA.
21. Schofield, M. J. and Hsieh, P. (2003) DNA mismatch repair: molecular mechanisms and biological function. Annu. Rev. Microbiol. 57, 579-608.
22. Shankar, S. and Tyagi, A. K. (1992) MhaAI, a novel isoschizomer of PstI from Mycobacterium habana recognizing 5′-CTGCA/G3′. Nucleic Acids Res. 20, 2891.
23. Shankar, S. and Tyagi, A. K. (1993a) Purification and characterization of restriction endonuclease Mgol from Mycobacterium gordonae. Gene 131, 153-154.
24. Shankar, S. and Tyagi, A. K. (1993b) MchAI and MchAII, two class-II restriction endonucleases from Mycobacterium chelonei. Gene 132, 119-123.
25. Starosciak, B. J. and Dobrzanski, W. T. (1980). Deoxyribonucleases of Streptococcus sanguis. Acta Microbiol. Pol. 29, 35-47.
26. Suri, R. (2005) The use of human deoxyribonuclease (rhDNase) in the management of cystic fibrosis. BioDrugs. 19, 135-144.
27. Winder, F. G and Lavin, M. F. (1971) Partial purification and properties of a nucleoside triphosphate-dependent deoxyribonuclease from Mycobacterium smegmatis. Biochim. Biophys. Acta. 247, 542-561.